Purpose. Determining prospects of introducing Callistephus chinensis cultivars of foreign breeding for further cultivation under conditions of the Central Forest-Steppe zone of Ukraine. Methods. Field, laboratory, analytical and statistical ones. Results. Ten C. сhinensis cultivars of theGerman breeding were tested under conditions of the Central Forest-Steppe zone of Ukraine. Biomorphological traits of the varieties of five sort types were identified, and their comparative analysis was conducted. Seasonal dynamics of growth and development of cultivars in the growing area was studied. Conclusions. Cultivars with short and mean duration phases of development can be grown under conditions of the Central Forest-Steppe zone of Ukraine by direct sowing that does not affect their seed productivity and sowing quality of seeds. Seeds of late cultivars can reach complete maturity only in case of seedling use. C. chinensis cultivars of the German breeding have successfully passed the initial test for suitability to introduction into the studied area.

Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordability for fungal species identification. However, Sanger sequencing fails to identify mixed ITS sequences in the case of mixed infections. To overcome this limitation, different high-throughput sequencing technologies have been explored. The nanopore-based technology is now one of the most promising long-read sequencing technologies on the market as it has the potential to sequence the full-length ITS region in a single read. In this study, we established a workflow for species identification using the sequences of the entire ITS region generated by nanopore sequencing of both pure yeast isolates and mocked mixed species reads generated with different scenarios. The species used in this study included Candida albicans (n = 2), Candida tropicalis (n = 1), Nakaseomyces glabratus (formerly Candida glabrata) (n = 1), Trichosporon asahii (n = 2), Pichia kudriavzevii (formerly Candida krusei) (n = 1), and Cryptococcus neoformans (n = 1). Comparing various methods to generate the consensus sequence for fungal species identification, the results from this study indicate that read clustering using a modified version of the NanoCLUST pipeline is more sensitive than Canu or VSEARCH, as it classified species accurately with a lower abundance cluster of reads (3% abundance compared to 10% with VSEARCH). The modified NanoCLUST also reduced the number of classified clusters compared to VSEARCH, making the subsequent BLAST+ analysis faster. Subsampling of the datasets, which reduces the size of the datasets by approximately tenfold, did not significantly affect the identification results in terms of the identified species name, percent identity, query coverage, percentage of reads in the classified cluster, and the number of clusters. The ability of the method to distinguish mixed species within sub-populations of large datasets has the potential to aid computer analysis by reducing the required processing power. The herein presented new sequence analysis pipeline will facilitate better interpretation of fungal sequence data for species identification.

Purpose. To analyze the types of license agreements and their difference from other civil agreements, and requirements for the execution and termination of a license agreement. Results. It was revealed that the current legislation provides an inexhaustible list of the types of transactions with respect to disposition of intellectual property rights. The group of agreements on disposition of intellectual property rights includes the intellectual property license. The legislation provides for three types of license agreements under the terms of the exclusive, non-exclusive and single license. The result of a license agreement is to provide the intellectual property rights for temporary use without losing the copyright holder of this object. Conclusions. Proper execution of license agreements between licensor and licensee promotes protection of rights of each of the parties to such agreements. Licensee will have a legitimate reasons to use the variety, the licensee may receive compensation for granting the right to use the variety.

Purpose. To analyze the types of license agreements and their difference from other civil agreements, and requirements for the execution and termination of a license agreement. Results. It was revealed that the current legislation provides an inexhaustible list of the types of transactions with respect to disposition of intellectual property rights. The group of agreements on disposition of intellectual property rights includes the intellectual property license. The legislation provides for three types of license agreements under the terms of the exclusive, non-exclusive and single license. The result of a license agreement is to provide the intellectual property rights for temporary use without losing the copyright holder of this object. Conclusions. Proper execution of license agreements between licensor and licensee promotes protection of rights of each of the parties to such agreements. Licensee will have a legitimate reasons to use the variety, the licensee may receive compensation for granting the right to use the variety.

Nomenclature of genus Vaccinium considered, as well as the application of names for indigenous and introduced species and denomination of fruit crops as derived from those species. System of binary species names for Vaccinium s.l. has been proposed as based on the Ukrainian generic name ‘lokhina’ that best complies with the principles for establishing generic names.

Nomenclature of genus Vaccinium considered, as well as the application of names for indigenous and introduced species and denomination of fruit crops as derived from those species. System of binary species names for Vaccinium s.l. has been proposed as based on the Ukrainian generic name ‘lokhina’ that best complies with the principles for establishing generic names.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. To find out the ecological importance of winter triticale varieties in the formation of species diversity of agro-ecosystems. Methods. Field, laboratoryones and mathematical and statistical analysis. Results. The authors studied ecological importance of winter triticale varieties of forest-steppe and Polissia ecotopes as determinants of agrobiocenosis in the structural and functional organization of species diversity. It was found that less favourable ecological niche for pests-phytophags is such winter triticale varieties and lines as ‘Slavetne’, ‘AD 256’, ‘Chaian’, ‘DAU 5’, for epiphytoparasites – ‘Vivate Nosivske’, ‘Pshenychne’, ‘Slavetne polipshene’, ‘Slavetne’, ‘Yaguar’, respectively. It is determined that varieties and lines of winter triticale such as ‘AD 256’, ‘Vivate Nosivske’, ‘Pshenychne’, ‘Slavetne polipshene’, ‘Slavetne’ show high biological ability to compete with synanthropic vegetation and form distinct associations of segetal plants. Conclusions. It was found that agrophytocenoses of the studied varieties of winter triticale under the conditions of forest-steppe, Polissia-forest-steppe and Polissia ecotops determined in movements structural and functional organization of species diversity of agroecosystems.

Purpose. To find out the ecological importance of winter triticale varieties in the formation of species diversity of agro-ecosystems. Methods. Field, laboratoryones and mathematical and statistical analysis. Results. The authors studied ecological importance of winter triticale varieties of forest-steppe and Polissia ecotopes as determinants of agrobiocenosis in the structural and functional organization of species diversity. It was found that less favourable ecological niche for pests-phytophags is such winter triticale varieties and lines as ‘Slavetne’, ‘AD 256’, ‘Chaian’, ‘DAU 5’, for epiphytoparasites – ‘Vivate Nosivske’, ‘Pshenychne’, ‘Slavetne polipshene’, ‘Slavetne’, ‘Yaguar’, respectively. It is determined that varieties and lines of winter triticale such as ‘AD 256’, ‘Vivate Nosivske’, ‘Pshenychne’, ‘Slavetne polipshene’, ‘Slavetne’ show high biological ability to compete with synanthropic vegetation and form distinct associations of segetal plants. Conclusions. It was found that agrophytocenoses of the studied varieties of winter triticale under the conditions of forest-steppe, Polissia-forest-steppe and Polissia ecotops determined in movements structural and functional organization of species diversity of agroecosystems.

Purpose. To study the effect of growth regulators EpinTM and Emistym C on yield and quality of strawberry berries of five varieties (Fragaria ananassa (Weston) Duсhesne ex Rozier) with various ripening time. Methods. Field, laboratory, analytical and statistical ones. Results. It was found that there were no impact of growth regulators EpinTM and Emistym C on the speed of phenological phases of strawberry plants development. It was experimentally determined the impact of mentioned substances on the yield of most studied varieties. Laboratory analysis has confirmed the changes in biochemical composition of strawberries. Conclusions. The use of growth regulators EpinTM end Emistym C had a positive effect on fruit yield and quality of studied strawberry varieties. Spraying with these substances increased yields by 14–51% as compared with the control. Content of sugars, dry matter and vitamin C in berries of most of varieties was increasing. At the same time the level of acidity in case of spraying with growth regulators was significantly reducing.

Purpose. To study the effect of growth regulators EpinTM and Emistym C on yield and quality of strawberry berries of five varieties (Fragaria ananassa (Weston) Duсhesne ex Rozier) with various ripening time. Methods. Field, laboratory, analytical and statistical ones. Results. It was found that there were no impact of growth regulators EpinTM and Emistym C on the speed of phenological phases of strawberry plants development. It was experimentally determined the impact of mentioned substances on the yield of most studied varieties. Laboratory analysis has confirmed the changes in biochemical composition of strawberries. Conclusions. The use of growth regulators EpinTM end Emistym C had a positive effect on fruit yield and quality of studied strawberry varieties. Spraying with these substances increased yields by 14–51% as compared with the control. Content of sugars, dry matter and vitamin C in berries of most of varieties was increasing. At the same time the level of acidity in case of spraying with growth regulators was significantly reducing.

Мета. Проаналізувати помилки, які допускають сторони в документах про передачу прав на сорти рослин при неправильному застосуванні заявниками та авторами сорту норм чинного законодавства в частині складання документів заявки на сорт рослини та додатків до неї; впорядкувати та розробити практичні рекомендації при укладанні правочинів про передачу прав на сорти рослин. Результати. Виявлено, що чинним законодавством передбачено укладання тільки двох видів договорів у сфері охорони прав на сорти рослин, якими не повністю забезпечуються права та інтереси сторін. Укладання договору про передачу права на подання заявки на сорт рослин є інструментом захисту прав заявників та підставою для прийняття її уповноваженим органом. Висновки. Оформлення документів заявки без помилок сприяє швидкому проведенню формальної експертизи та прийняттю позитивного для заявника рішення. Правильне оформлення трудових угод між селекціонером (автором) та роботодавцем сприяє захисту прав кожного з учасників таких угод.

Species in the order Rhytismatales M.E. Barr ex Minter (Leotiomycetes, Ascomycota) develop on a wide range of host plants, but prefer conifers, such as species of Cupressaceae and Pinaceae. Conifers, the largest group of gymnosperms, show a high diversity in China. In this study, the species diversity of Rhytismatales on twigs of conifers is investigated based on specimens newly collected in China. Morphological characteristics combined with multi-gene phylogenetic analysis (ITS, nrLSU, and mtSSU rDNA) revealed 18 new species, belonging to six new genera (Abiomyces, Cryptococcomyces, Labivalidus, Neotherrya, Pseudococcomyces, and Stipamyces) and three known genera (Hypoderma, Hypohelion, and Tryblidiopsis). Additionally, seven new combinations are proposed. The findings underscore the complexity of fungal taxonomy within Rhytismatales and the importance of considering multiple criteria for accurate classification. The study also explores the importance of host specificity for genus and species delimitation within the order. A key to genera and species of Rhytismatales on twigs of conifers worldwide is provided.

Purpose. Comparative evaluation of varieties of tomato bred by Kyiv experimental station of the Institute of vege­tables and melons (IVM) NAAS of Ukraine with allowance for different fruit shapes. Methods. Field, laboratory, statistical ones. Results. Since varietal characteristics can greatly influence the course of the growth process, the experiment included phenological observations of plants growth and development, biometric measurements of plants and fruits, productivity records, besides, biochemical values of fruits with various shape were defined (flat-round and plum-shaped fruits). Varieties with the highest yield, best biochemical values and earlier fruit ripening were identified. Conclusions. Among the samples of tomato with flat-round fruits, Zoren variety had the shortest time interval from mass germination up to early ripening of fruits, Myroliubivskyi variety – in the group of plum-shaped fruits. In the first group Atlasnyi variety had the highest yield, in the se­cond – Khoriv variety. Atlasnyi (flat-round fruits) and Oberig (plum-shaped fruits) varieties had the best biochemical values. Varieties with plum-shaped fruits excelled samples with flat-round fruits at biochemical composition.

Purpose. Comparative evaluation of varieties of tomato bred by Kyiv experimental station of the Institute of vege­tables and melons (IVM) NAAS of Ukraine with allowance for different fruit shapes. Methods. Field, laboratory, statistical ones. Results. Since varietal characteristics can greatly influence the course of the growth process, the experiment included phenological observations of plants growth and development, biometric measurements of plants and fruits, productivity records, besides, biochemical values of fruits with various shape were defined (flat-round and plum-shaped fruits). Varieties with the highest yield, best biochemical values and earlier fruit ripening were identified. Conclusions. Among the samples of tomato with flat-round fruits, Zoren variety had the shortest time interval from mass germination up to early ripening of fruits, Myroliubivskyi variety – in the group of plum-shaped fruits. In the first group Atlasnyi variety had the highest yield, in the se­cond – Khoriv variety. Atlasnyi (flat-round fruits) and Oberig (plum-shaped fruits) varieties had the best biochemical values. Varieties with plum-shaped fruits excelled samples with flat-round fruits at biochemical composition.