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Use of partially immobilized membrane ATPase in heavy metals determination
1998
Nikezic, G. (Institut za nuklearne nauke Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju i endokrinologiju) | Horvat, A. | Jovanovic, D. | Vasic, V. | Vujisic, Lj. | Nedeljkovic, N. | Radak, B. | Kopecni, M.
Previous studies clearly indicated that membrane enzymes - ATPases i.e. Na, K-ATPase and Mg-ATPase, responded to presence of various organic and inorganic pollutants. In this work effect of mercury and cadmium on these enzymes activities was investigated in synaptic plasma membranes partially immobilized on microliter plate. Comparing those activities with the control enzyme activities obtained with native and partially immobilized mambranes it was concluded that: a. both metals exerted a concentration-dependent inhibition of investigated enzymes, b. for partially immobilized membranes estimated half maximum inhibition (IC50) values for Na, K-ATPase were IC50 (Hg) = 0.9 micromol/cubic cm, IC50 (cd) = 35 micromol/cubic cm and for Mg - ATPase IC50 (Hg) = 3.5 micromol/cubic cm, IC50 (Cd) = 36 micromol/cubic cm; for native membranes IC50 for Na,K-ATPase were IC50 (Hg) =3.3 micromol/cubic cm, IC50 (Cd) = 2 micromol/cubic cm and for Mg ATPase IC50 (Hg) = 2.3 micromol/cubic cm, IC50 (Cd) = 0.2 mmol/cubic cm. Obtained results indicate avaibility of microtitar plates for partially immobilization of membranes with aim to form a new biosensor for heavy metals detection.
显示更多 [+] 显示较少 [-]Phosphatase activity and microbiological parameters as indicators of the canal waters pollution by the oil industry wastewater [Yugoslavia]
1998
Petrovic, O. | Radnovic, D. | Gajin, S. | Matavulj, M. | Trivunovic, V. (Prirodno-matematicki fakultet, Novi Sad (Yugoslavia). Institut za biologiju)
On the basis of the enzymatic (phosphatase) activity and microbiological analysis as well, the influence of the oil refinery "Vital" - Vrbas (Serbia, Yugoslavia) wastewater to the recipient DTD (Danube-Tisza-Danube) canal water from the microbiological point of view was determined. In addition the efficiency of the factory's waste water purification plant was estimated. The obtained results of the complex microbiological-enzymological analyses indicated a good water quality at the point where the refinery's water supplies plant is situated (site 1). Very low water quality was determined downstream (site 4) and upstream (site 2) from the inflow of the refinery's wastewater's inflow. The later results might indicated the influence of an unknown pollutant in the region. However, the obtained O/H index value suggests that the self-purification ability of the canal waters is still preserved.
显示更多 [+] 显示较少 [-]Microbiological and chemical characterization of waste mud from the waste water purification plants of the plant oil refinery
1997
Trivunovic, V. (Prirodno-matematicki fakultet, Novi Sad (Yugoslavia). Institut za biologiju) | Petrovic, O. | Jarak, M. | Galonja, T. | Petkovic, K.
This paper deals with microbiological and chemical analysis of waste mud, produced in wastewater purification plants of the oil refinery. After digest, mud is removed to the filter press. Compressed mud is placed and stored on the storage yard. Content of organic, anorganic and oil matters and also heavy metals contents were determined. Microbiological analysis comprised determination of total count of aerobic organotrophic organisms and many physiological groups of bacteria. As the indicator of the general biochemical activity, enzymatic (dehydrogenase and phosphatase) activities were determined. Waste mud was mixed with soil (chernozem) in 20%, 40% and 60% amount relations. Changes of activities and quantitative fluctuations of the present microflora were observed after 15 and 30 days. Gained results should enable assessing of using-ability of waste mud in soil-quality improvement, in the sense of its utilization as the fertilizer.
显示更多 [+] 显示较少 [-]Antioxidant responses to simulated acid rain and heavy metal deposition in birch seedlings
1997
Koricheva, J. | Roy, S. | Vranjic, J.A. | Haukioja, E. | Hughes, P.R. | Hanninen, O. (Laboratory of Ecological Zoology, Department of Biology, University of Turku, FIN-20014 Turku (Finland))
Effect of cadmium on germination, amylases and rate of respiration of germinating pea seeds
1996
Chugh, L.K. | Sawhney, S.K. (Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar 125004 (India))
Effects of reactive hydrocarbons and hydrogen peroxide on antioxidant activity in cherry leaves
1995
Terry, G.M. | Stokes, N.J. | Lucas, P.W. | Hewitt, C.N. (Institute of Environmental and Biological Sciences, Lancaster University, Lancaster, LA1 4YQ (United Kingdom))
Effects of 2,4-Diamin on some parameters of protein and carbohydrate metabolisms in the serum, muscle and liver of Cyprinus carpio
1999
Oruc, E.O. | Uner, N. (University of Cukurova, Faculty of Arts and Sciences, Department of Biology, Balcali, Adana, Turkey)
Photosynthetic inhibition and superoxide dismutase activity in soybean cultivars exposed to short-term ozone fumigations
1993
Sheng, W.S. | Chevone, B.I. | Hess, J.L. (Department of Plant Pathology, Physiology and Weed Science, Virginia Technical University, Blacksburg, VA 24061 (USA))
Analyses of enzyme activities and other metabolic criteria after five years of fumigation
1990
Bender, J. | Manderscheid, R. | Jager, H.J. (Institut fur Produktions- und Okotoxikologie des Bundesforschungsanstalt fur Landwirtschaft, Braunschweig-Volkenrode (FAL), W-3300 Braunschweig (Germany))
Toxic action of zinc on growth and enzyme activities of rice Oryza sativa L. seedlings
1984
Nag, P. | Nag, P. | Paul, A.K. | Mukharji, S. (Pl. Physiol. Lab., Dep. Bot., Univ. Calcutta, Calcutta 700 019 (India))