Membrane separation processes find applications in an array of fields as they use far less energy and chemical agents than competing processes. However, a major drawback of membrane technology is that biofilm formation alters membrane performances. Preventing biofilm formation is thus a pivotal challenge for larger-scale development of membrane processes. Here, we studied the comparative antibacterial activities of different inorganic membranes (ceramic and zeolite-coated ceramic with or without copper exchange) using several bacterial strains (Escherichia coli, Staphylococcus aureus, and Bacillus subtilis). In static conditions, alumina plates coated with Cu-exchanged zeolite showed significant bactericidal activity. In dynamic mode (circulation of a contaminated nutrient medium), there was no observable bacterial adhesion at the surface of the Cu-exchanged material. These results confirm the antifouling properties of the Cu-mordenite layer due to both the increased hydrophilicity and antibacterial properties of the active layer.Tests performed with tubular filtration membranes (without copper exchange) showed a significant decline in membrane hydraulic properties during filtration of culture media containing bacteria, whereas copper-exchanged membranes showed no decline in hydraulic permeability. Filtration tests performed with concentrated culture media containing spores of B. subtilis led to a significant decrease in membrane hydraulic permeabilities (but less so with Cu-exchanged membranes). The surfaces showed less effective global antifouling properties during the filtration of a concentrated culture medium due to competition between bacterial growth and the bactericidal effect of copper. Analyses of copper leached in solution show that after a conditioning step, the amount of copper released is negligible.

Plants have been naturally gifted with mechanisms to adjust under very high or low nutrient concentrations. Heavy metal toxicity is considered as a major growth and yield-limiting factor for plants. This stress includes essential as well as non-essential metals. MicroRNAs (miRNAs) are known for mediating post-transcriptional regulation by cleaving transcripts or translational inhibition. It is commonly agreed that an extensive understanding of plant miRNAs will significantly help in the induction of tolerance against environmental stresses. With the introduction of the latest technology like next generation sequencing (NGS), a growing figure of miRNAs has been productively recognized in several plants for their diverse roles. These miRNAs are well-known modulators of plant responses to heavy metal (HM) stress. Data regarding metal-responsive miRNAs point out the vital role of plant miRNAs in supplementing metal detoxification by means of transcription factors (TF) or gene regulation. Acting as systemic signals, miRNAs also synchronize different physiological processes for plant responses to metal toxicities. In contrast to practicing techniques, using miRNA is a greatly helpful, pragmatic, and feasible approach. The earlier findings point towards miRNAs as a prospective target to engineer heavy metal tolerance in plants. Therefore, there is a need to augment our knowledge about the orchestrated functions of miRNAs during HM stress. We reviewed the deterministic significance of plant miRNAs in heavy metal tolerance and their role in mediating plant responses to HM toxicities. This review also summarized the topical developments by identification and validation of different metal stress-responsive miRNAs.

Dissolved organic matter (DOM), as the most active organic carbon in the soil, has a coherent affinity with heavy metals from inherent and exogenous sources. Although the important roles of DOM in the adsorption of heavy metals in soil have previously been demonstrated, the heterogeneity and variability of the chemical constitution of DOM impede the investigation of its effects on heavy metal adsorption onto soil under natural conditions. Fresh DOM (FDOM) and degraded DOM (DDOM) from sugarcane rind were prepared, and their chemical properties were measured by Fourier-transform infrared spectrometry (FTIR), excitation-emission matrix (EEM) fluorescence spectroscopes, nuclear magnetic resonance (NMR), and molecular weight distribution (MWD). They were also used in batch experiments to evaluate their effects on the adsorption of Cu(II) onto farmland red soil. Based on our results, the chemical structure and composition of DDOM greatly varied; compared with FDOM, the C/O ratio (from 24.0 to 9.6%) and fluorescence index (FI) (from 1.4 to 1.0) decreased, and high molecular weight (>10 kDa) compounds increased from 23.18 to 70.51%, while low molecular weight (<3 kDa) compounds decreased from 56.13 to 12.13%; aromaticity and humification degree were markedly enhanced. The discrepancy of FDOM and DDOM in terms of chemical properties greatly influenced Cu(II) adsorption onto red soil by affecting DOM-Cu(II) complex capacity. The FDOM inhibited the adsorption of Cu(II), while DDOM promoted adsorption, which was significantly influenced by soil pH. Maximum adsorption capacity (Q ₘ) was 0.92 and 5.76 mg g⁻¹ in the presence of FDOM and DDOM, respectively. The adsorption process with DDOM could be better described by the Langmuir model, while that with FDOM was better described by the Freundlich model. The impacts caused by the dynamic changes of the chemical properties of DOM under natural conditions should therefore be considered in the risk assessment and remediation of soils contaminated with heavy metals.

The study was aimed to evaluate the levels of chlorpyrifos (CPF) pollution in agricultural soil of Punjab, India, its detrimental effects on acetylcholinesterase (AChE) activity in rat brain and bioremediation of soils polluted with CPF using indigenous and adapted bacterial lab isolate. The analysis revealed that soil samples of Bathinda and Amritsar regions are highly contaminated with chlorpyrifos showing 19 to 175 mg/kg concentrations of CPF. The non-targeted animals may get poisoned with CPF by its indirect dermal absorption, inhalation of toxic fumes and regular consumption of soiled food grains. The study indicated that even the lowermost concentrations of CPF, 19 and 76 mg/kg of soil found in the Amritsar and Bathinda regions respectively can significantly inhibit the AChE activity in rat brain within 24 h of its treatment. This represents the antagonistic effect of CPF on AChE which is a prime neurotransmitter present in all living beings including humans. In light of this, an attempt was made to remediate the polluted soil, a major reservoir of CPF, using Pseudomonas sp. (ChlD), an indigenous bacterial isolate. The culture efficiently degraded 10 to 100 mg/kg chlorpyrifos supplemented in the soil and utilized it as sole source of carbon and energy for its growth. Thus, this study provides a detailed insight regarding the level of CPF pollution in Punjab, its detrimental effects on mammals and bio-based solution to remediate the sites polluted with CPF.

Extracellular polymeric substances (EPS) are key components of the cyanobacterium Microcystis aeruginosa and play an important role in cyanobacteria blooms formation. Here, we analyzed the effects of 48-h exposure to nanosized CeO₂ (n-CeO₂), CuO (n-CuO), and ZnO (n-ZnO) on the production and composition of EPS of M. aeruginosa. Toxicity experiments revealed that soluble nanoparticles (NPs) (n-ZnO, n-CuO) demonstrated higher toxicity to cells and caused membrane damage. The production of LB-EPS increased by 34.48, 20.09, and 46.33 %, and TB-EPS increased by −5.78, 22.3, and −2.67 % in the presence of n-CeO₂, n-CuO, and n-ZnO NPs, respectively, and polysaccharides are the main incremental portion compared with protein and humic acids. Three-dimensional excitation–emission fluorescence spectra revealed the enhancement of fulvic–humic-like and disappearance of tyrosine aromatic substances in TB-EPS compared with the slight changes observed in LB-EPS. Fourier-transform infrared spectroscopy illustrated the susceptibility of −NH₂ and double-bonded carbon and oxygen in amides to three types of NPs. These results improve our understanding of the potential influence of NPs on the aggregation behaviors of cyanobacteria and formation process of cyanobacteria blooms. Graphical abstract ᅟ

Vertical distribution of metals (Cu, Zn, Cr, Fe, Mn, Pb, Ni, Cd, and Li) in four sediment core samples (C₁, C₂, C₃, and C₄) from Anzali international wetland located southwest of the Caspian Sea was examined. Background concentration of each metal was calculated according to different statistical approaches. The results of multivariate statistical analysis showed that Fe and Mn might have significant role in the fate of Ni and Zn in sediment core samples. Different sediment quality indexes were utilized to assess metal pollution in sediment cores. Moreover, a new sediment quality index named aggregative toxicity index (ATI) based on sediment quality guidelines (SQGs) was developed to assess the degree of metal toxicity in an aggregative manner. The increasing pattern of metal pollution and their toxicity degree in upper layers of core samples indicated increasing effects of anthropogenic sources in the study area.

In order to illuminate the mechanism of the interaction of polycyclic aromatic hydrocarbon (PAH) with different benzene rings and arsenic (As) in As hyperaccumulator, Pteris vittata L., the uptakes of PAHs were investigated using hydroponics simulation and localizations of PAHs in the plant were determined using two-photon laser scanning confocal microscopy (TPLSCM). The results showed that the total As concentration in different parts of P. vittata decreased in the presence of PAHs with increased numbers of benzene rings: 38.0–47.4% for benzo(a)pyrene (BaP, five rings), 20.5–35.9% for pyrene (PYR, four rings), and 13.7–16.6% for fluorine (FLU, three rings). BaP and PYR concentrations increased, while FLU concentration decreased in the presence of As. The results of TPLSCM revealed that PAHs distributed in epidermal cells of roots, xylem, and endothelial cells of rachis, epidermis, and stomatal cells of pinnae; however, the fluorescence intensity of BaP and PYR were higher than FLU significantly in plant. This study provided important basis to further research on interactive effects of PAHs and As in the P. vittata. These findings were important to understand the mechanisms of PAH and As translocation and distribution by P. vittata.

The current study was aimed to investigate the impact of THI on productive and reproductive indices of PE and F₁ crosses (50% PE and 50% Italian buffaloes) and back crosses (BC) (75% PE and 25% Italian buffaloes) under hot conditions. In this study, 8385 records used PE (1914, 1518, and 1737), F₁ (387, 447, and 657), and BC (495, 585, and 645) for low, medium, and high THI, respectively. The high THI reduced the conception rate after first insemination in PE, F₁, and BC ((odds ratio, OR) = 1.187, P = 0.007; 2.361, <0.0001 and 1.603, <0.0001, respectively) when compared with low THI. But, stillbirth and calving condition were not significantly influenced by different THI levels in BC and F₁. BC was more bearable to the harsh environment; they possessed the highest incidence of conception after first insemination (72.70, 72.60, and 62.40%), producing live calves (98.50, 100, and 99.40%) with easy calving condition (98.50, 100, and 99.40%) when compared to PE and F₁ at different levels of THI, respectively. The average daily milk yield and peak of milk production were decreased in PE (4.02 and 5.12%), BC (13.33 and 10.95%), and F₁ (25.29 and 12.20%) from low to high THI. However, BC revealed no significant changes in days open, dry period, calving interval, gestation length, service per conception, and the first service post-partum at different levels of THI when compared with PE and F₁. Therefore, rearing BC is recommended for improving buffalo productive and reproductive performance under hot conditions.

When compared to the European guidelines, PM₁₀ (particulate matter up to 10-μm size) concentrations in Algeria are often exceeding the maximum limits, and in general, no information exists on the compounds bound on its surface. The objective of this study was to measure the dioxin-like activity of polychlorinated dibenzodioxines and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (PCBs) in the PM₁₀ fraction at the Sour El Ghozlane cement plant in Algeria. PM₁₀ samples (n = 23) were taken between 24 March and 15 April 2013, using a medium volume sampler and 47-mm PTFE filters. The 24-h samples were dried to determine the PM₁₀ content and afterward extracted, cleaned up, and analyzed with the dioxin-responsive element–chemical-activated luciferase gene expression (DRE-CALUX) bioassay. Our results showed that the measured bioanalytical equivalents (BEQs) were similar to those in other international industrial sites worldwide. The PCDD/Fs and dioxin-like PCBs (dl-PCBs) were positively correlated (rho = 0.6, p = 0.002), indicating that they have similar sources. Furthermore, samples from March showed higher PCDD/F and dl-PCB BEQs and humidity but lower temperatures compared to samples from April, while there was no difference in the PM₁₀ concentrations between the two months. These results reveal that PM₁₀ alone is not a good proxy and that meteorological conditions are an important factor in assessing dioxin-like pollution in the atmosphere. It seems that, at present, there is no health hazard through direct airborne human exposure to dioxin-like pollutants in PM₁₀ from this site. However, it is important to monitor these POPs for a longer period of time and also to gain more insight in their distribution between the particulate and gas phase in relation to meteorological conditions.

Urban surface waters face several stressors associated with industry and urban water management. Over much of the past century, the wastewater treatment in Berlin, Germany, relied on inefficient sewage farms, which resulted in severe eutrophication and sediment contamination in the recipient surface waterbodies. A prominent example is Lake Tegel, where a multitude of management measures were applied in the last decades for the purpose of ecosystem restoration. In this study, we analyzed sediment cores of three lakes with X-ray fluorescence spectroscopy: Lake Tegel, Lake Großer Wannsee, which is environmentally similar but has a different management history, and Lake Userin, which serves as a reference located in a nature protection area. Multivariate statistical methods (principal component analysis, k-means clustering, and self-organizing maps) were used to assess the sediment quality and to reconstruct the management history of Lake Tegel. Principal component analysis established two main gradients of sediment composition: heavy metals and lithogenic elements. The impact of the management measures was visualized in the lake sediment composition changing from high abundance of heavy metals and reducing redox conditions to less-impacted sediments in recent layers. The clustering techniques suggested heterogeneity among sites within Lake Tegel that probably reflect urban water management measures. The abundance of heavy metals in recent lake sediments of Lake Tegel is similar to a lake with low urban impact and is lower than in Lake Großer Wannsee suggesting that the management measures were successful in the reduction of heavy metals, which are still a threat for surface waters worldwide.