Water kefir is a fermented probiotic beverage produced by fermentation of dried sugary fruits and sugar using water kefir grains. Sucrose is used as a sugar source in the traditional production of water kefir. The aim of this study is to determine the effects of grape molasses, honey and unrefined sugar on water kefir grains instead of sucrose. In the study, 4 groups were formed: grape molasses (10 g), honey (10 g), unrefined sugar (10 g) and refined sugar (10 g). 2 g of water kefir grain and 500 mL of drinking water were used for each group. And each group samples were fermented for 25°C 48 hours. The study continued for 5 weeks. The pH values and the titratable acidity values of water kefir samples and the weight of water kefir grains were determinated once every two day, while microbiological analyzes (Lactobacillus spp., Lactococcus spp. and yeast contents) and ethanol content of samples were determinate once every 15 days. In this study; it has been observed that the use of different sugars has no significant effect on the weights of the water kefir grains. In addition, it was determined that the Lactococcus spp. and yeast amounts in the water kefirs were higher in the samples produced by using honey and grape molasses. As a result, it was revealed that grape molasses, honey and unrefined sugar could be used as fermentation substrate in production of water kefir. At the same time, water kefir can be shown as an alternative fermented product for vegan consumers with allergies to dairy products.

Bu araştırma Göller Yöresi (Türkiye) florasında farklı lokasyonlarda doğal olarak yetişen Cnidium silaifolium (Jacq.) Simonkai’nin meyve uçucu yağ oranı ve bileşenlerinin belirlenmesi amacıyla 2014 yılında yürütülmüştür. Uçucu yağ oranı clavenger tipi hidro-distilasyon cihazında, uçucu yağ bileşenleri ise GC/MS’de belirlenmiştir. Aksu (1,120 m) ve Dedegöl (1,540 m) lokasyonlarından alınan meyvelerin uçucu yağ oranları sırasıyla %0,50 ve %0,67, uçucu yağı oluşturan bileşen sayıları ise sırasıyla 38 ve 29 olarak tespit edilmiştir. Her iki lokasyonda da uçucu yağı oluşturan önemli bileşenlerin β-Elemene (%22,30-29,79), Trans-Caryophyllene (%11,85-21,88), Germacrene-D (%9,74-17,31), α-Humulene (%5,22-5,85) ve δ-Cadinene (%4,82-6,66) olduğu belirlenmiştir. Çalışmada rakımın uçucu yağı oluşturan bileşenler ve oranları üzerine önemli derecede etki gösterdiği, düşük rakımda bulunan bazı bileşenlerin yüksek rakımlarda bulunmadığı tespit edilmiştir.

This study aims to investigate the views and awareness level of nutrition and dietetics undergraduates about genetically modified organisms (GMO). A total of 228 university students participated in the research (130 female, 98 male students). During data collection, 8 open-ended questions and attitude scale questionnaire (30 questions) were applied in order to determine the demographic features and GMO knowledge of students. Students’ replies to questions; “Do you think GMO technology is beneficial or harmful? If it's beneficial, why? If it is harmful, why?” 76.8% stated that they found it harmful. To the question, “if it is harmful why?” students replied, “they cause cancer”. There is a statistical difference between the answers of first and third-year students. To the question, “have you heard about the precautions that were taken by the ministry? If yes, do you think they are sufficient?” 76.80% students stated that they were not aware of the precautions that were taken by the ministry. 53 participants replied to this question as “I have an idea about this.” But to the question “Is it sufficient?” they stated that they are not sure about it. There is a statistical difference between graduates of Vocational High School and Anatolian High School. In this assessment, it was seen that the basic knowledge of students about GMO is enough, but they experience confusion. Most of the students believe that GMO technology is harmful and has some conceptual errors. Positive and negative sides of GMO technology should be expressed clearly. It was concluded that course curriculums should include topics that will increase knowledge and awareness related to GMOs. Additionally, courses can be given selectively in the field of biotechnology so that confusion about GMO’s can be eliminated.

Plants need different macro and micro nutrient elements to grow and reproduce. Their malnutrition results in unhealthy growth. The soils of Naqadeh area, Iran are deficient in micronutrient elements. The aim of the study was to evaluate the effects of micronutrients application on some qualitative and quantitative parameters of economically important monogerm sugar beet cv. Sonja. The plants were foliar sprayed with iron, boron, zinc, and manganese. The studied traits were potassium, sodium, nitrogen, extraction coefficient, sugar percent, recoverable sugar, alkalinity, molasses, root yield, and white sugar recovery yield. The effect of micronutrients was significant on all studied traits. The highest extraction coefficient of 89.31 was related to the foliar application of iron (Fe) and the highest recoverable sugar percent (16.91%) was obtained from Zn application. Also, the highest mean root yield (74.120 t ha-1) and white sugar yield (12.137 t ha-1) were noted after foliar treatments of boron (B) and Fe, respectively.

Industries, as a source of pollution, have a considerable impact on aquatic ecosystems due to the diversity in the composition of their wastewater. In this study, it is aimed to determine the heavy metal pollution caused by the wastewater of milk products factory, paint factory and textile factory in the Organized Industrial Zone of Kahramanmaraş province. For this purpose, seasonal concentrations of some heavy metals (Fe, Cu, Zn, Cr, Ni, Cd, As and Hg) have been determined in wastewaters of milk products factory, paint factory and textile factory and in the region where these wastewaters discharge in the Erkenez Stream. The seasonal variation of heavy metals in all stations, generally the lowest concentrations of all elements were determined in winter and the highest concentrations were determined in summer. When the heavy metal contents of milk products factory, paint factory and textile factory wastewaters were compared, the highest values were found in textile factory wastewater. When all stations are taken into consideration, according to USEPA and water quality criteria, it is determined that the wastewaters of milk products factory, paint factory and textile factory and the area of the Erkenez Stream, where these wastewaters are discharged, were very dirty in term of heavy metals. When these results are taken into consideration, it is clear that the wastewater of these factories causes serious heavy metal pollution in the Erkenez Stream.

According to World Health Organization (WHO), an estimated 600 million people fall ill after eating contaminated food and 420 000 die every year. Although various foods can serve as sources of foodborne illness, meat has been at the forefront of societal concerns in recent years. Meat is source of some foodborne pathogens which have an important role on human health. In this study, it was aimed to examine the microbiological quality of raw bovine meat samples sold in Tokat province and to evaluate the results in the context of food safety. For this purpose, 18 raw meat samples purchased from butchers and markets were analyzed for total mesophilic aerobic bacteria, total psychrophilic aerobic bacteria, yeasts-molds, lactic acid bacteria, B. cereus, S. aureus, C. perfringens, total coliform, and fecal coliform. The presence of E. coli, E. coli O157:H7, L. monocytogenes and Salmonella spp. were also investigated in these samples. The lowest and highest values determined for the parameters were as follows: total mesophilic aerobic bacteria 1.46×104-1.26×107 CFU/g, total psychrotrofic aerobic bacteria 1.01×104-2.65×106 CFU/g, yeasts-molds 3.00×103-1.70×104 CFU/g, lactic acid bacteria 2.70×103-3.60×104 CFU/g, B. cereus ˂102-7.20×104 CFU/g, S. aureus 2.60×103-2.57×105 CFU/g, C. perfringens ˂102-9.20×103 CFU/g, total coliform 3.80×101-2.90×104 MPN/g, and fecal coliform

Over the years, the growing concerns about the negative impact of anti-microbial growth promoters has triggered researches into the use of phytogenic feed additives in poultry production in order to ascertain better performance of birds as well as safety in the food chain. Phytogenic feed additives are compound derived from varieties of plants, its parts, extracts and essential oils which possess active substances that are of significant importance. Some of its functions include anti-microbial and anti-oxidative effects on the poultry feeds and carcass as well as enhancement of flavour of feeds. Despite the beneficial influences of PFAs, numerous controversial issues have emerged on the appropriate dosage to be used in poultry production. This may be due to the differences in edaphic and climatic actions on plants available in various part of the world and stage of harvesting of plants which makes standardizing inclusion levels of phytogenic feed additives in poultry diet seemingly difficult. However, it is crystal clear that phytogenic feed additives qualify as a better substitute to anti-microbial growth promoters as it is safe and ecologically friendly.

Sardina pilchardus is a multiple spawning fish, i.e., the species that spawns periodically during the spawning period. In order to examine the reproduction biology of the species, the specimens were obtained in the known spawning period indicated by the current literature obtained from fishers who were commercially fishing in December 2018 and January-February 2019. After the fork length and total length together with their weight measurements, the specimens were dissected in the laboratory. The adult females’ gonads were then fixed in 4% formalin solution for fecundity. A total of 170 individuals was examined and the values of their fork length, total length and weight were computed as 9.7-13.4 cm (mean: 11.16±0.81), 10.5-15 cm (mean: 12.3±0.89), 8.06-23.7 g (mean: 12.7±2.93), respectively. 44 of the supplied specimens were male (25.9%), 122 female (71.8%) and 4 (2.3%) uncertain, thus, the female: male ratio was determined as 2.77:1. According to χ2 test results, a statistically significant difference was observed among individuals. For the specimens examined, the fork length-weight relationship was calculated as W = 0.0098FL2.96, while total length-weight relationship was W = 0.0071TL2.97. In order to calculate the fecundity, sub-samples were taken from the anterior, median and posterior parts of 15 ovaries in the ration of 2 - 5% of the ovary weight and the mature oocytes were counted. As a result, the species’ fecundity was found to be between 4,600-9,800 (6,110±1,755). The relationship between total length and fecundity was computed as W= 1,640.7 TL-13,907 and a linear relationship was determined.

Turunçgillerin küçük mücevheri olarak nitelendirilen kamkat (Fortunella spp.) Rutaceae familyasının Fortunella cinsi içerisinde yer almaktadır. Anavatanı Çin olup, 19. Yüzyıl’da Amerika ve Avrupa’ya getirilmiş ve subtropik iklimlere adapte edilmiştir. Dünyada Çin, Japonya ve Amerika’da yaygın olarak yetiştirilmektedir. Yapılan çalışmalarda kamkatın içerdiği askorbik asit, karotenoidler, uçucu yağlar, diyet lif ve flavonoidler gibi biyoaktif bileşenlerin sağlık üzerine olumlu etkileri olduğu bildirilmiştir. Kamkat meyvesi taze tüketiminin yanında reçel, marmelat, şarap, likör ve meyve çayı gibi farklı gıda ürünlerine işlenerek de değerlendirilmekte, uçucu yağı ve ekstraktları gıda ve kozmetik sektöründe kullanılmaktadır. Sağlık üzerine olumlu etkileri olduğu anlaşılan kamkatın ülkemizde son yıllarda yetiştiriciliği ve üretimi konusundaki çalışmalar artış göstermiştir. Bu derlemede kamkat meyvesinin önemli biyoaktif bileşenleri ve biyolojik aktiviteleri ile ilgili çalışmalar özetlenmiştir.

In our study, validation was carried out at 24 and 200 mg/kg enrichment for residue analysis of nitrate active substance only in lettuce samples. Analyses were performed by using high performance liquid chromatography (HPLC) in lettuce, spinach, salad and arugula samples. In order to eliminate the matrix effect, matrix calibration was used, ie calibration of lettuce samples. Validation of the method was performed with criteria such as precision, repeatability, repeatability limit, reproducibility, repeatability limit, accuracy, linearity of calibration and recovery. The calibration curve was found as linear in the range of 0.2-40 mg/kg. The recovery from the samples is between 97.3-100.2 %. The repeatability and reproducibility values for the two concentrations were 1.89 (matrix+24 ppm) and 3.65 (matrix+200 ppm), 3.951 (matrix+24 ppm) and 3.456 (matrix+200 ppm), respectively. The repeatability and reproducibility limit values are within acceptable limits. Accuracy, precision, combined uncertainty and extended uncertainty (95% confidence: k=2) were found as 0.015 (matrix+24 ppm) and 0.004 (matrix+200 ppm), 0.039 (matrix+24 ppm) and 0.006 (matrix+200 ppm), 0.041 (matrix+24 ppm) and 0.007 (matrix+200 ppm), 0.082 (matrix+24 ppm) and 0.014 (matrix+200 ppm). In our study, the highest nitrate ratio was determined in spinach with 3486 mg/kg in total 102 samples. Lettuce following spinach (2825 mg/kg) showed lower nitrate content, while lower concentrations of nitrate were detected in iceberg (1985 mg/kg) and arugula (1870 mg/kg), respectively. The results may be useful in estimating body intake levels for research on the effects of nitrite and nitrate on human health in vegetable producers and in our daily diet. In addition, the nitrate content of the vegetables analysed in the study is an indicator of the use of nitrogenous fertilizers in the Mediterranean region, but showed no problem for human and animal health.