The health and vitality of callus growth is one of the prerequisites for the success of further in vitro studies. This study investigated the efficiency of different percentage (0%, 10%, 20%, 30%, and 40%) of vermicompost tea as an organic substance on in vitro callus growth in sainfoin. Morpho-physiological responses of calli to vermicompost tea measured under in vitro conditions. As a result of this investigation, a combination of plant growth regulators (4 mg/l BAP and 0.5 mg/l NAA) with 20% of vermicompost tea causing significant callus initiation and growth in sainfoin stem explants. Under the light of these scientific findings, vermicompost tea might be used as an organic bio stimulant for efficient callus growth and complementary to commercial chemical hormones in sainfoin. This research is important due to it can contribute positively to the plant species that are difficult in terms of callus growth and plant regeneration in tissue culture.

The aim of this study was to evaluate the clinic-histopathologic characteristics and to compare the morphometric measurements of healthy and subclinical laminitic claws of dairy cattle at different ages and weights. Non-lame 60 Holstein feet randomly collected from the slaughterhouse were evaluated. The effects of age, body-weight, claw location (right front lateal or right front medial etc), and presence of laminitis were investigated. The claws’ conformation were evaluated morphometrically with ten measurements (toe length, toe height, outer and inner edges of the claw, heel height, the length of heel, the length of diagonal front wall, dorsal hoof angle, the width and the length of the sole). The claws were classified as normal or laminitic according to the histopathologic findings. The clinical findings of laminitis was confirmed on 71.2% of the claws (n=66). The toe length, toe height, the height of outer and inner edges of the claw, heel height, the length of heel, the length of diagonal front wall were smaller in laminitic claws. The dorsal hoof angle of healthy claws were bigger and statistically significant than the laminitic claws. Small haemorrhagic areas were determined in the parietal corium in the laminitic claws comparing to macroscopically healthy claws. The histopathologic characteristics of the corium of laminitic claws involve the hyperaemia, haemorrhages, oedema, thrombosis of capillaries and presence of mononuclear cell infiltration in dermis, stretching epidermal lamella, necrosis of epithelial cells and detachment of the lamellar basement membrane. According to this study results, contrary to literature, there was not a reliable relation between some changes in morphological structure of the claws and the presence of the laminitis were observed.

Ensuring the olive oil quality and authenticity has become a great importance for both traditional and emerging olive oil producing countries. The chemical composition in olive oil heavily varies depending on the olive cultivar and its growing region, the agronomic applications, the olive oil production methods and the process and storage conditions. With the help of some analytical techniques and data evaluation methods, it is possible to grade olive oils in terms of their differences. This research examines particularly fatty acid composition of commercial olive oils (2017/2018 season) with mass detector coupled with gas chromatography (GC/MS). Results were evaluated for grading of them according to IOC regulations based on cultivar (ripe or unripe Ayvalik and Memecik), production (organic, stone mill, cold press, two or more centrifugation systems, filtered or unfiltered) and packing type (transparent or dark glass bottle and plastic bottle), and also their geographic origin (Ayvalik and Edremit towns, the Cunda Island, North Aegean region or South Aegean Region). According to overall data processing, virgin olive samples could be successfully distinguished in terms of theirs geographic origin and cultivar roots. Moreover, it was also explained that the effect of process and package type for grading of olive oils as ‘extra virgin’ or ‘virgin’.

In this study, the effects of carvacrol addition which is a natural preservative to apple juice samples contaminated with Z. bailii which is an osmotolerant yeast and shows resistance against high sugar concentration, low acidity, ethanol content and pasteurization process, were investigated on some physicochemical and bioactive properties and also changes in yeast numbers during storage period. For this purpose, the response surface methodology was applied and storage time (1-41 days), storage temperature (4-20°C), sodium benzoate amount (0-0.1%) and carvacrol amount (0-750 ppm) were selected as processing variables. Significant changes in physicochemical properties were observed due to yeast viability occurred during the storage of apple juice samples. The increase in the amount of carvacrol caused a decrease in the number of yeasts about 7 log level and prevented the spoilage of fruit juices. However, the brix value of the samples without carvacrol showed a decrease of 50% as a result of yeast activity and the fruit juice could not be consumed. Total phenolic content of the sample was in the range of 136.7-645.7 mg GAE/L and the lowest total phenolic content was determined for the run 11 having no carvacrol while the highest total phenolic content was for the sample added with the highest carvacrol level. As a result of the optimization process, it was observed that the deterioration activities of Z. bailii could be prevented to a great extent by the addition of maximum amount of carvacrol.

The present study investigated the effects of the prebiotic inulin on the growth parameters, digestive enzyme activities and antioxidant status of rainbow trout (Oncorhynchus mykiss). After acclimation, fish (34±0.22 g; n = 135) were assigned into 9 tanks (15 fish per tank) and triplicate groups were fed a control diet (G-0) or diets containing 1% (G-1) or 2% (G-2) inulin. At the end of the trial (8 weeks), growth factors (final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), Economic conversion ratio (ECR), digestive enzyme activities (pepsin, trypsin, amylase and lipase) and antioxidant status superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) level of liver tissue were assessed. At the end of the study highest survival rate was observed in the fish fed with 1% G-1 inulin group. Similarly, digestive enzyme activities were significantly higher dietary G-1 group. Subsequently, elevated growth performance (final weight, SGR and FCR) was observed in trout fed with 1% compared to the control group. The effect of the dietary inulin on digestion was partly observed by assaying the activity of pepsin, intestinal amylase, trypsin and lipase. Apart from pepsin activity, results indicated that dietary supplementation of inulin is beneficial and may also have differential effects upon digestive enzyme activities. Antioxidant enzyme activity of SOD and CAT in liver tissue was generally higher in the supplemented inulin diets than in the control and significantly higher in the 1% inulin group. A significant decrease in MDA level of tissue was observed in all inulin supplemented groups when compared to the control group. These results indicate that inulin be considered as a beneficial dietary supplement for growth performance, digestive enzyme activities and antioxidant status of rainbow trout.

Çiftçilerin tarım sektörünün doğal yapısından kaynaklanan birçok unsuru dikkate alarak çeşitli ürünler arasından kendilerine en uygun olanını nasıl seçtikleri ve üretimine nasıl karar verdiklerinin belirlenmesi önemlidir. Bu araştırmanın amacı, Samsun ili Bafra ilçesindeki sebze yetiştiricilerinin ürün desenlerine karar verirken etkilendikleri faktörleri belirlemektir. Bu amaca ulaşmak için basit tesadüfi örnekleme yöntemi ile belirlenen 122 çiftçi ile anket yapılmıştır. Çiftçilerin ürün desenlerine karar verirken etkilendikleri faktörleri belirlemek amacıyla, incelenen literatürden yararlanılarak ve araştırmacı tarafından yapılan pilot çalışmalardan hareketle sosyal ve kişisel, ekonomik, fiziksel ve iklim koşullarından kaynaklı faktörleri içine alan 67 madde tespit edilmiştir. Çiftçilerin düşüncelerini yansıtan ifadeler Likert tipi beşli bir ölçeklendirmeye göre puanlanmıştır. Araştırmada istatistiksel işlemler sırasına göre iç tutarlılık katsayılarının belirlenmesi, açıklayıcı faktör analizi, doğrulayıcı faktör analizi olarak gerçekleştirilmiştir ve bulgular sunulmuştur. Açıklayıcı faktör analizi sonucunda toplam varyansın %57,74’ünü açıklayan; ürün özellikleri, fiziksel özellikler, işletme özellikleri, doğal faktörler, sulama özellikleri ve işgücü özellikleri olarak altı faktör grubu ortaya çıkmıştır. Analiz sonuçlarından elde edilen bulgulara göre çiftçilerin ürün desenlerini planlarken etkilendikleri en önemli faktör ürün özelliği ile ilişkilidir. Ancak, çiftçiler üretecekleri ürünlere karar verirken sadece ürün özelliklerinden değil aynı zamanda ellerinde olmayan farklı koşullardan etkilenmektedirler. Dolayısıyla çiftçilerin kararlarını etkileyen faktörler arasında davranış özelliklerinin de dikkate alınması gerektiği düşünülmektedir.

This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizer samples) collected from retailers located various districts of Istanbul. Conventional microbiological methods and PCR procedures were used for Listeria monocytogenes and Staphylococcus aureus while only PCR procedures were used for the analysis of Toxoplasma gondii. Also PCR specifity and sensitivity for all the positive samples were calculated. According to the results, Listeria monocytogenes was detected in 9 samples (9%) and Staphylococcus aureus was detected in 36 (36%) samples while all the samples were negative for Toxoplasma gondii. PCR sensitivity results were quite specific and accurate for both Listeria monocytogenes and Staphylococcus aureus. It was concluded that salad and appetizers may be seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.

Salinity stress is one of the most important and common abiotic stress factors that cause significant physiological and metabolic changes in plants, negatively affecting plant growth and development, and causing decrease in product quality and quantity. The elucidation of the molecular control mechanisms associated with salt stress tolerance is based on the activation and /or inactivation of various stress-related genes. Salt Overly Sensitive (SOS) tolerance mechanism under salt stress is of great importance in terms of salt tolerance of the plants. Although this mechanism has been studied for many years, the physiological changes that the plants give as a result of mutation of the genes in the pathway under different levels of sodium chloride (NaCl) during development have not been examined comparatively. In this study, we found that the Arabidopsis thaliana sos1-1 mutant plant showed sensitivity to 10 mM NaCl while the sos3-1 and hkt1-1 mutants showed tolerance. The sos1-1, sos3-1 and hkt1-1 mutants showed increasing sensitivity when NaCl was applied beyon 50 mM of concentration. In addition, plants did not show significant sensitivity for 1 day of stress application, while significant effects were observed in plant root length when exposed to salinity for 3 to 4 days. Col-0, hkt1-1 and sos3-1 roots treated with low levels of NaCl for a short term were positively affected in length. In the light of these results, the amount and duration of salt stress is very critical in Arabidopsis thaliana's responses to the stress and determination of molecular tolerance pathways.

Bu çalışmada kerevit (Astacus leptodactylus Eschscholtz) yemine farklı oranlarda katılan selenyumun hepatopankreas ve gonad dokularında paraoksonaz (PON) ve arilesteraz (ARE) enzim aktivitelerine etkisi araştırılmıştır. Çalışmada, toplam selenyum düzeyi 0,3, 0,6, 0,9 ve 1,2 mg/kg yem olan sırasıyla; Kontrol (K), Deneme 1 (D1), Deneme 2 (D2) ve Deneme 3 (D3) yemleri oluşturulmuştur. Araştırma yemlerinin ham protein ve toplam enerji düzeyleri eşitlenmiştir. Çalışmada ebatları 2,0 × 2,0 × 1,0 m olan 12 adet havuz kullanılmıştır. Her bir havuza 75 dişi-25 erkek olacak şekilde toplam 1200 adet kerevit stoklanmıştır. Üç tekrarlı olarak yürütülen çalışmada kerevitler günde 2 öğün olmak üzere 9 ay süreyle yemlenmiştir. Kerevitlerden aylık olarak alınan doku örneklerinde PON ve ARE enzim aktiviteleri tespit edilmiştir. Deneme süresince hepatopankreas ve gonad dokularındaki PON ve ARE enzim aktivitelerinde istatistiksel olarak önemli farklılıklar bulunmuştur. Kerevitlerin dokularında üreme sezonu ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerinin arttığı belirlenmiştir. Bu artışın kontrol grubuna kıyasla D1, D2 ve D3 gruplarında istatistiksel olarak farklı olduğu bulunmuştur. Sonuç olarak, selenyumun A. leptodactylus’un çiftleşme dönemi, yumurtlama dönemi ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerini olumlu yönde etkilediği görülmüştür.

Bu araştırma, farklı silajlık mısır çeşitlerinin verim ve bazı özelliklerini belirlemek amacıyla 2018 yılında Isparta Uygulamalı Bilimler Üniversitesi Ziraat Fakültesi, Eğitim, Araştırma ve Uygulama Çiftliği’nde yürütülmüştür. Çalışmada materyal olarak 6 silajlık mısır çeşidi (DKC721, Hido, Kilowat, Kolosseus, LG30709 ve PNR31143) kullanılmıştır. Denemeler, tesadüf blokları deneme desenine göre 3 tekrarlamalı olarak kurulmuştur. Araştırmada yeşil ot verimi, bitki boyu, sap, yaprak ve koçan oranları, yaprak sayısı, koçan uzunluğu ve koçan çapı değerleri belirlenmiştir. Varyans analiz sonuçlarına göre çeşitler arasında yeşil ot verimi, bitki boyu, yaprak sayısı, koçan boyu ve koçan çapı değerleri bakımından %1 düzeyinde, koçan oranı bakımından ise %5 düzeyinde istatistiksel olarak önemli farklılıklar bulunurken, sap ve yaprak oranları açısından çeşitler arasında gözlemlenen farklılıkların istatistiksel olarak önemsiz olduğu belirlenmiştir. İncelenen özellikler bakımından, yeşil ot verimi 8624,9-10681,0 kg/da, bitki boyu 234,3-294,3 cm, yaprak sayısı 13,0-15,2 adet/bitki, koçan uzunluğu 20,7-26,0 cm ve koçan çapı 46,5-55,6 mm arasında değişmiştir. Özellikle verim ve sap, yaprak ve koçan oranları açısından Hido çeşidi öne çıkarken bunu Kolosseus ve LG30709 çeşitlerinin takip ettiği gözlemlenmiştir. DKC721 çeşidinin ise incelenen özellikler bakımından geride kaldığı söylenebilir.