Large seasonal water-level fluctuations may influence isotopic signatures of primary producers and the types and amounts of these potential food sources accessible to aquatic fauna of Poyang Lake, the largest freshwater lake in China. In this study, the isotopic signatures of primary producers and consumers were determined, stable carbon and nitrogen isotope analysis and mixing models were combined to investigate the influence of water levels on the diet and isotopic composition of Poyang Lake fish and invertebrates. Five potential food sources (seston, benthic organic matter, aquatic macrophytes, attached algae, and terrestrial plants), 4 species of invertebrates, and 10 species of fish were collected from the lake area during dry and wet seasons between January 2009 and April 2010. The δ 13C values of invertebrates and most fish were within the range of δ 13C values of the potential food sources for both seasons. The δ 13C values of invertebrates and most fish were lower in the dry season than in the wet season, whereas the δ 15N values exhibited different patterns for different species. Mixing models indicated that the most important food sources for common lake fauna were seston in the dry season and aquatic macrophytes and terrestrial plants in the wet season. The fauna were more omnivorous in the wet season than in the dry season. The food web dynamics of Poyang Lake are strongly influenced by changes in the abundance and accessibility of different basal food sources that occur because of seasonal flood pulses. The trophic links within the aquatic communities of Poyang Lake are modified by water-level fluctuations.

The use of food quotients (FQ) calculated from dietary records is proposed for predicting respiratory quotients (RQ) that are needed for calculating free-living energy expenditure determined by the doubly-labeled water method which predicts respiratory CO2 production. This approach is based on the fact that, over the relatively long periods of double-isotope measurements (10-20 days), FQ (which can be obtained from a single 4-day measurement of dietary composition) must equal RQ in subjects in energy balance. It is shown that FQ can easily be adjusted to account for anabolism or catabolism for subjects not in energy balance. Typical RQ values are reported for various population sub-groups residing in the United Kingdom (omnivorous adults, vegetarians, vegans, Asian immigrants, and weaned and breast- and bottle-fed infants).(wz)

For the purpose of examining on role of irrigation and cultivation system to paddy ecosystem, we study species and number of Odonata larvae and analyze the stable isotope ratios of the Odonata larvae and the plankton/detritus in water of paddy fields and irrigation ponds. As a results, cultivation system clearly effects to composition of species and number of Odonata larvae. delta sup(13)C indicates that Orthetrum albistylum speciosum larvae and Coenagrionidae spp. larvae which are dominant species in paddy water, depend on specified food sources, each other. delta sup(15)N shows that the trophic level of Odonata larvae is higher than that of the plankton/detritus in paddy water. It is considered that analyze the stable isotope ratios application is effective to examine water ecosystem and food web in paddy.

Wild Atlantic salmon smolts were captured during spring out-migration in the Northwest Miramichi River, New Brunswick, Canada, and placed on an isotopically distinct hatchery diet to determine the relative contributions of growth and metabolic turnover to isotopic change. As expected for an ectothermic species, growth explained a large amount of isotopic variation in changing stable carbon ratios of muscle tissue (average r ²= 0.46) for the 3 years of study. Turnover rates of muscle carbon in all 3 years in growing fish (0.24–0.66 month⁻¹) were higher than previously reported values for other ectothermic species, but there was little evidence for isotopic change in non-growers (average r ²= 0.041, p > 0.1). It is unlikely that non-growers had consumed any of the hatchery diet over a 2-month period, thus preventing them from acquiring the new carbon isotopic signature. This period of food deprivation resulted in nitrogen-15 enrichment in liver relative to muscle (p= 0.003). It is advised that future isotope studies of metabolic turnover rates in ectotherms be conducted on slow-growing animals over a long time period. This would serve to avoid the obscuring effects of growth on isotopic change, and provide stronger comparisons to endothermic tissue turnover rates.

Riverine transport of dissolved inorganic carbon (DIC) from land to the ocean is an important carbon flux that influences the carbon budget at the watershed scale. However, the dynamics of DIC in an entire river network has remained unknown, especially in mountainous Japanese watersheds. We examined the effects of watershed land use and geology on the transports of inorganic carbon as well as weathered silica (Si) and calcium (Ca) in the Iwaki River system where agricultural and residential areas have developed in the middle and lower parts of the watershed. The concentration and stable carbon isotope ratios (δ¹³C) of DIC showed the longitudinal increase of ¹³C-depleted inorganic carbon along the river. As a result, most streams and rivers were supersaturated in dissolved CO₂ that will eventually be emitted to the atmosphere. The possible origin of ¹³C-depleted carbon is CO₂ derived from the decomposition of organic matter in agricultural and urban landscapes, as well as from in-stream respiration. In addition, agricultural and urban areas, respectively, exported the large amount of dissolved Si and Ca to the rivers, suggesting that CO₂ increased by respiration accelerates the chemical weathering of silicate and carbonate materials in soils, river sediments, and/or urban infrastructure. Furthermore, riverine bicarbonate flux is likely to enter shell carbonates of Corbicula japonica, an aragonitic bivalve, in the downstream brackish lake (Lake Jusan). These results revealed that the flux of DIC from the human-dominated watersheds is a key to understanding the carbon dynamics and food-web structure along the land-to-river-to-ocean continuum.