The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

Moskwa, B.; Bien, J.; Cabaj, W.; Kořinková, K.; Koudela, B.; Stefaniak, J.

The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

2009

The aim of this study was to compare the performance of four different ELISA procedures in diagnosing Trichinella spp. infections in human outbreaks. The main differences between the various procedures were: antigen concentration, dilutions of human serum sample, the type of conjugate used, and time of conjugate incubation. Serum samples were collected from 23 individuals involved in an outbreak. Results obtained in the four tested ELISA procedures differed in the rate of positive results obtained for the examined outbreak. Western blot confirmed the ELISA-positive results. Reactions to the specific 41-45kDa and 55kDa bands were obtained with all positive sera.

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AGROVOC Keywords

accuracy adolescent aged animals antibodies antigens blood diagnostic techniques disease outbreaks disease outbreaks epidemiology health helminth humans humans immunology methodology methods molecular weight parasitology poland trichinella trichinosis western blotting

Bibliographic information

Published in

Veterinary parasitology

Volume 159 Issue 3 Pagination 312 - 315 ISSN 0304-4017

Publisher

Elsevier

Other Subjects

Animal parasitic nematodes; Child; Enzyme-linked immunosorbent assay; And safety; Human medicine; Immunoglobulin g; Disease detection; Diet and diet-related diseases; Trichinellosis; Young adult; Enzyme-linked immunosorbent assay; Adult; Immunoglobulin g; Middle aged; Disease diagnosis; Female; Male; Serologic tests

Language

English

Note

In the special issue: selected papers presented at the 12th international conference on trichinellosis - plitvice lakes national park, croatia / edited by alvin a. gajadhar, h. ray gamble, delores hill, albert marinculic and dante zarelnga.

Includes references

2019-12-06

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

Data Provider

This bibliographic record has been provided by National Agricultural Library

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Links

DOI http://dx.doi.org/10.1016/j.vetpar.2008.10.069

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