Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

Alberti, S.; Fornaro, M.

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

1990

Alberti, S. | Fornaro, M.

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford (1,2) to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.

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AGROVOC Keywords

analysis animals cell lines dna electrophoresis fibroblasts genes genetics guanidines humans humans leukocytes leukocytes mice mice organic nitrogen compounds purification spermatozoa spermatozoa thiocyanates transfection transfection

Bibliographic information

Published in

Nucleic acids research

ISSN 0305-1048

Publisher

Center for Leather, Rubber, and Plastics

Other Subjects

Cell line; L cells; Isolation & purification; Agar gel; Biochemical techniques; Southern; Blotting; Isothiocyanates; Male; L cells; Genome; Indicators and reagents

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-27

Format: MODS

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Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

1990

AGROVOC Keywords

Bibliographic information