Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

Alberti, S.; Fornaro, M.

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

1990

Alberti, S. | Fornaro, M.

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford (1,2) to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.

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Ключевые слова АГРОВОК

analysis animals cell lines dna electrophoresis fibroblasts genes genetics guanidines humans humans leukocytes leukocytes mice mice organic nitrogen compounds purification spermatozoa spermatozoa thiocyanates transfection transfection

Библиографическая информация

Опубликовано в

Nucleic acids research

ISSN 0305-1048

Издатель

Center for Leather, Rubber, and Plastics

Другие темы

Cell line; L cells; Isolation & purification; Agar gel; Biochemical techniques; Southern; Blotting; Isothiocyanates; Male; L cells; Genome; Indicators and reagents

Язык

Английский

Примечание

2019-12-05

Тип

Journal Article; Text

В АГРИСе с: 2024-02-27

Формат: MODS

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Эту запись предоставил National Agricultural Library

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Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

1990

Ключевые слова АГРОВОК

Библиографическая информация