Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure
1990
Alberti, S. | Fornaro, M.
Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford (1,2) to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.
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书目信息
出版者
Center for Leather, Rubber, and Plastics
其它主题
Cell line; L cells; Isolation & purification; Agar gel; Biochemical techniques; Southern; Blotting; Isothiocyanates; Male; L cells; Genome; Indicators and reagents
语言
英语
注释
2019-12-05
类型
Journal Article; Text
2024-02-27
MODS