Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

Kelling, C.L.; Kennedy, J.E.; Rump, K.K.; Stine, L.C.; Paul, P.S.; Partridge, J.E.

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

1991

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide tide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

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AGROVOC Keywords

analysis animals cattle chemistry electrophoresis gel genetics immunology live vaccines metabolism monitoring purity ribonucleases rna rna screening

Bibliographic information

Published in

American journal of veterinary research

Volume 52 Issue 8 Pagination 1237 - 1244 ISSN 0002-9645

Other Subjects

Bovine viral diarrhea virus; Viral vaccines; Viral; Bovine viral diarrhea virus 1; Two-dimensional; Biochemical techniques; Nucleotide mapping; Bovine viral; Diarrhea viruses; Exoribonucleases; Oligonucleotides

Language

English

Note

2019-12-04

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

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This bibliographic record has been provided by National Agricultural Library

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