Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

Kelling, C.L.; Kennedy, J.E.; Rump, K.K.; Stine, L.C.; Paul, P.S.; Partridge, J.E.

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

1991

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide tide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

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Mots clés AGROVOC

analysis animals cattle chemistry electrophoresis gel genetics immunology live vaccines metabolism monitoring purity ribonucleases rna rna screening

Informations bibliographiques

Publié dans

American journal of veterinary research

Volume 52 Numéro 8 Pagination 1237 - 1244 ISSN 0002-9645

D'autres materias

Biochemical techniques; Two-dimensional; Exoribonucleases; Viral; Oligonucleotides; Bovine viral diarrhea virus 1; Bovine viral diarrhea virus; Diarrhea viruses; Bovine viral; Nucleotide mapping; Viral vaccines

Langue

anglais

Note

2019-12-04

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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