Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

Kelling, C.L.; Kennedy, J.E.; Rump, K.K.; Stine, L.C.; Paul, P.S.; Partridge, J.E.

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

1991

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide tide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

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Ключевые слова АГРОВОК

analysis animals cattle chemistry electrophoresis gel genetics immunology live vaccines metabolism monitoring purity ribonucleases rna rna screening

Библиографическая информация

Опубликовано в

American journal of veterinary research

Том 52 Выпуск 8 Нумерация страниц 1237 - 1244 ISSN 0002-9645

Другие темы

Biochemical techniques; Two-dimensional; Exoribonucleases; Viral; Oligonucleotides; Bovine viral diarrhea virus 1; Bovine viral diarrhea virus; Diarrhea viruses; Bovine viral; Nucleotide mapping; Viral vaccines

Язык

Английский

Примечание

2019-12-04

Тип

Journal Article; Text

В АГРИСе с: 2024-02-28

Формат: MODS

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