Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies

Srikumaran, S.; Kluever, E.A.; Onisk, D.V.; Hariharan, K.

Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies

1991

Srikumaran, S. | Kluever, E.A. | Onisk, D.V. | Hariharan, K.

Bovine immunoglobulin isotype-specific murine monoclonal antibodies were used in sandwich radioimmunoassays to detect and quantitate bovine IgG1, IgG2, IgM, and IgA in culture fluids. The concentrations of bovine immunoglobulins in unknown samples were extrapolated from standard curves generated with bovine monoclonal immunoglobulins. The lowest detection limits for the bovine immunoglobulin isotypes ranged from 65 to 270 ng/ml.

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AGROVOC Keywords

analysis animals antibodies cattle cattle chemistry culture media immunoglobulins methods monoclonal antibodies quantitative analysis radioimmunoassay

Bibliographic information

Published in

American journal of veterinary research

Volume 52 Issue 2 Pagination 243 - 246 ISSN 0002-9645

Other Subjects

Monoclonal; Immunoglobulin g; Radioimmunoassays; Veterinary; Immunoglobulin a; Immunoglobulin a; Immunoglobulin g; Igg1; Diagnostic use; Immunoglobulin m; Immunoglobulin m; Igg2

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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