Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies

Srikumaran, S.; Kluever, E.A.; Onisk, D.V.; Hariharan, K.

Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies

1991

Srikumaran, S. | Kluever, E.A. | Onisk, D.V. | Hariharan, K.

Bovine immunoglobulin isotype-specific murine monoclonal antibodies were used in sandwich radioimmunoassays to detect and quantitate bovine IgG1, IgG2, IgM, and IgA in culture fluids. The concentrations of bovine immunoglobulins in unknown samples were extrapolated from standard curves generated with bovine monoclonal immunoglobulins. The lowest detection limits for the bovine immunoglobulin isotypes ranged from 65 to 270 ng/ml.

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Ключевые слова АГРОВОК

analysis animals antibodies cattle cattle chemistry culture media immunoglobulins methods monoclonal antibodies quantitative analysis radioimmunoassay

Библиографическая информация

Опубликовано в

American journal of veterinary research

Том 52 Выпуск 2 Нумерация страниц 243 - 246 ISSN 0002-9645

Другие темы

Diagnostic use; Immunoglobulin g; Igg1; Igg2; Radioimmunoassays; Monoclonal; Immunoglobulin m; Immunoglobulin m; Veterinary; Immunoglobulin a; Immunoglobulin a; Immunoglobulin g

Язык

Английский

Тип

Text; Journal Article

В АГРИСе с: 2024-02-28

Формат: MODS

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