Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies
1991
Srikumaran, S. | Kluever, E.A. | Onisk, D.V. | Hariharan, K.
Bovine immunoglobulin isotype-specific murine monoclonal antibodies were used in sandwich radioimmunoassays to detect and quantitate bovine IgG1, IgG2, IgM, and IgA in culture fluids. The concentrations of bovine immunoglobulins in unknown samples were extrapolated from standard curves generated with bovine monoclonal immunoglobulins. The lowest detection limits for the bovine immunoglobulin isotypes ranged from 65 to 270 ng/ml.
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书目信息
American journal of veterinary research
卷
52
期
2
页码
243
- 246
ISSN
0002-9645
其它主题
Igg2; Immunoglobulin m; Igg1; Immunoglobulin a; Immunoglobulin m; Diagnostic use; Immunoglobulin a; Radioimmunoassays; Veterinary; Immunoglobulin g; Immunoglobulin g; Monoclonal
语言
英语
类型
Text; Journal Article
2024-02-28
MODS
