Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

Tanaka, Katsunori; Yokoi, Satomi; Morimoto, Koji; Iwata, Takayuki; Nakamoto, Yuka; Nakayama, Kaori; Koyama, Kōichi; Fujiwara, Takeshi; Fukase, Koichi

Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

2012

Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.

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AGROVOC Keywords

animals antibodies antibodies avidin avidin biotin cells chemistry fluorescence humans immunology methods microscopy ultrastructure

Bibliographic information

Published in

Bioorganic & medicinal chemistry

Volume 20 Issue 6 Pagination 1865 - 1868 ISSN 0968-0896

Publisher

Elsevier Ltd

Other Subjects

Cell line; Confocal; Biotinylation; Biotinylation; Cell membrane; Fluorescent dyes; Hela cells; Labeling techniques; Cell survival; Staining and labeling

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

Data Provider

This bibliographic record has been provided by National Agricultural Library

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Links

DOI http://dx.doi.org/10.1016/j.bmc.2011.12.043

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