Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

Tanaka, Katsunori; Yokoi, Satomi; Morimoto, Koji; Iwata, Takayuki; Nakamoto, Yuka; Nakayama, Kaori; Koyama, Kōichi; Fujiwara, Takeshi; Fukase, Koichi

Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

2012

Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.

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Mots clés AGROVOC

animals antibodies antibodies avidin avidin biotin cells chemistry fluorescence humans immunology methods microscopy ultrastructure

Informations bibliographiques

Publié dans

Bioorganic & medicinal chemistry

Volume 20 Numéro 6 Pagination 1865 - 1868 ISSN 0968-0896

Editeur

Elsevier Ltd

D'autres materias

Cell line; Confocal; Biotinylation; Biotinylation; Cell membrane; Fluorescent dyes; Hela cells; Labeling techniques; Cell survival; Staining and labeling

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

Découvrez la collection de ce fournisseur de données dans AGRIS

Liens

DOI http://dx.doi.org/10.1016/j.bmc.2011.12.043

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