Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

Tanaka, Katsunori; Yokoi, Satomi; Morimoto, Koji; Iwata, Takayuki; Nakamoto, Yuka; Nakayama, Kaori; Koyama, Kōichi; Fujiwara, Takeshi; Fukase, Koichi

Cell surface biotinylation by azaelectrocyclization: Easy-handling and versatile approach for living cell labeling

2012

Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.

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Palabras clave de AGROVOC

animals antibodies antibodies avidin avidin biotin cells chemistry fluorescence humans immunology methods microscopy ultrastructure

Información bibliográfica

Publicado en

Bioorganic & medicinal chemistry

Volumen 20 Edición 6 Paginación 1865 - 1868 ISSN 0968-0896

Editorial

Elsevier Ltd

Otras materias

Cell membrane; Confocal; Biotinylation; Cell survival; Staining and labeling; Cell line; Fluorescent dyes; Labeling techniques; Biotinylation; Hela cells

Idioma

Inglés

Tipo

Text; Journal Article

En AGRIS desde: 2024-02-28

Formato: MODS

Proveedor de Datos

Este registro bibliográfico ha sido proporcionado por National Agricultural Library

Descubra la colección de este proveedor de datos en AGRIS

Enlaces

DOI http://dx.doi.org/10.1016/j.bmc.2011.12.043

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