Investigation of histological structures of liver of fattening broiler chickens after application of concentrate of E and F vitamins produced on the basis of rapeseed oil was realized in the conditions of the Republic of Belarus in order to evaluate their efficiency for prophylaxis of massive hepatic necrosis. Research results showed that application of the vitamins on the basis of rapeseed oil promoted the support of structural properties of hepatic cells that proved their higher antioxidant efficiency in comparison with synthetic vitamin analogues

There was presented a territorial analysis of epizootic situation on rabies in the administrative districts and regions of the Republic of Belarus in the period from 1986 up to 2008. The situation of strain was stated on the territory of Vitebsk (28, 4%) and Minsk (20,2%) regions. The most stable epizootic situation was stated in Shumilin, Braslavsk and Vitebsk districts of Vitebsk region, as well as in Vologin and Minsk districts of Minsk region. Only in 1990 and 1997 there were stated no cases of rabies in Brest and in 1996 in Mogilev regions. In other years no regions were free from this infection. Economic damage from rabies cases in agricultural animals in period 1986-2008 was over 2 milliard of Belarusian rubles.

The physical and biological properties of a produced in the conditions of the Republic of Belarus nonspecific blood serum of animals according to an improve technology corresponded to all necessary standards. The prophylactic efficiency of nonspecific blood serum of animals was 80%, that was on 10% higher than serum of convalescents. Therapeutic effectiveness of nonspecific blood serum was 90%, that was on 8% higher than serum of convalescents

In the conditions of the Republic of Belarus there was developed a concept for differentiation of eimeria and helminthes using a system of identification indexes as mathematical expressions of morphometric correlations of eimeria oocystae and helminth eggs morphology. There was developed a method for identification eimeria species on the basis of two-dimensional mathematical analysis of oocysts using a new identification index which represented a duplication proportion of contour perimeter to surface area of an oocyst.

Data on development of an antigen on the basis of larvae of warble flies grubs (Hypodenna bovis) for diagnostic of spontaneous cattle hypodermosis by means of the indirect fluorescent (IFA) test and with application of blood serum and milk samples realized in the conditions of the Republic of Belarus were presented. Specificity and sensitivity of experimental sample of antigen for revealing of spontaneously infestated with larvae of warble flies with IFA test at hypodermotosis has made about 100%.

Development of highly efficient antigens for an early immunodiagnosis of cattle fascioliasis (Fasciola hepatica) and studying of its main properties was realized in the conditions of the Republic of Belarus. Received in result of the experiment antigens possessed high specificity and were suitable for use in fascioliasis immunodiagnostics. Research results showed that use of indirect fluorescent (IFA) test and immunodiffusion test for fasciolasis immunodiagnostics of cattle excludes false positive results caused by tuberculosis and hypodermotosis (p less than 0,001).

In the conditions of the Republic of Belarus there was studied a therapeutic effectiveness and influence on milk productivity of 22% Timbendazole after spontaneous infestation of cows with fascioliasis (Fasciola hepatica). As a result of studies the Timbendazole 22% proved to be highly efficient against Fasciola hepatica in cattle – from 92,9% up to 100% (in comparison with Albendatimum-100 granulated material the efficiency of which was 88-90%). As a result of cows treatment by anthelmintics – Timbendazole 22% and Albendatimum-100 against Fasciola hepatica there was stated the substantial increasing of milk productivity of animals (on 23,4 and 11,0%, respectively) in comparison with animals which were not administratec with mentioned above preparations.

Results of treatment and preventive maintenance of mastitis of lactating nonmilking cows and heifers with the help of a new probiotic preparation Lactosan realized in the conditions of the Republic of Belarus were presented. The developed new environmentally safe antimastitis preparation Lactosan contained lactic acid bacteria Lactobacillus plantarum and Lactobacillus acidophilus in amount not less than10E7 CFU/cm3 did not render irritating action on mucous membranes, did not cause a sensitization and according to its toxicity indexes could be concern to low-hazardous substances. Therapeutic effectiveness of Lactosan against subclinical mastitis was 84,2-94,1%; in case of serous and catarrhal mastitis – 63,2-68,4%; and after two therapy regimen – 79,0-84,2%.

General characteristic of parasitologic situation at the exclusion zone, some aspects of new conditions influence on separate systematic groups of mammal parasites Polesye State Radiation and Nature Reserve of the Republic of Belarus were presented. Research of helminth fauna and helminthological situation was realized by an example of 1 European bison, 7 mooses, 20 roe deers, 26 boars, 25 wolves, 50 racoon dogs, 6 foxes and 3 beavers. Scatoscopy of the animal excrements was realized by Kalantaryan method with saturated solution of sodium saltpeter, Berman method. Research results revealed 66 helminth species belonging to 4 classes and 34 families. There were found 10 parasite species at bison, 16 – at mosses, 17 – at roe deers, 16 – at boars, 8 – at deers, 23 – at wolves, 13 – at raccoon dogs, 13 – at foxes, 9 – at badgers and 1 – at beavers. Analyze of helminthofauna of the analyzed area showed that the nature reserve had its own helminthes complex. A lot of stated helminth species were parasites with a wide range of hosts, including domestic animals, and cattle. Wolves, foxes and raccoon dogs were hosts of some custodies invasions. Carnivorous animals and murine rodents were hosts of trichinosis invasion, which was stated at 41,2 % of raccoon dogs, 33,3 % of foxes and 16,7 % of wolves. Also trichinas were stated at bank vole (1,3%), yellow-necked mouse (2,7%), and house mouse (16,6%)

Determination of influence of long acting antihelminthics (Aversictini boluses and boluses with albendazole) and influence of usual antihelminthics (farmatsin, alverm, fenbendavet) on first stomachanimal digestion, as well as on quantity and quality composition of microflora and microfauna in first stomach and large bowel was realized in the conditions of the Republic of Belarus. In course of the experiment there were used 6 groups of calves infested with helminths (Neoascaris vitulorum, Strongylata sp.). Research result showed that different antihelminth preparations to a greater or lesser degree rendered negative influenced on quantity and quality composition of microorganisms in first stomach and large bowel of cattle. Alverm and boluses with albendazole rendered the least influence on microflora and microfauna that could be explained by low toxicity of active substance (albendazole) of the above preparations. Aversictini boluses, fenbendavet, and farmastin changed the composition of microorganisms. It could be explained by high toxicity of preparations containing aversictini which rendered influence not only of gastro-intestinal tract but also on the whole organism. Active ingredient of mentioned above preparations (Aversektin C), being the product of microbial synthesis, rendered the antagonist action on the normal microflora of digestive tract. Negative influence of fenbendavet was caused by the chemical composition of the preparation and neurotoxic action of fenbendazol