Barley lines evaluation for malting quality by using hordein protein pattern
1999
Parichart Sangkasa-ad
Hordein patterns of two seed sets of 134 barley lines from 22 crosses from breeding program in Thailand were evaluated by the SDS-PAGE technique. The first set of seed derived from 112 lines of F4 seeds from 17 crosses and 22 lines of F5 seeds from 5 crosses. The second set of seed derived from plants grown from seed of the first set seed. The objectives of this study were to observe relationship between hordein protein patterns and malting quality characters of barley lines in Thailand. Hordein patterns evaluated in this study were B-hordein and C-hordein in which 28 bands of C-hordein were arranged to be 14 C-hordein patterns and 29 bands of B-hordein were arranged to be 17 B-hordein patterns. Results from the evaluation of hordein pattern revealed that stability of hordein patterns were observed in F4 seeds and most of hordein patterns were also observed in the successive generation. Both B-hordein and C-hordein patterns showed some relationship to some favorable malting quality characteristics. Most of favorable malting quality characteristics namely malt yield, malt extract, extract yield, diastatic power and DP/TN had rather the same pattern in both B-hordein and C-hordein. On the other hand, character of seed size namely seed plumpness and 1,000 seed weight had rather the same pattern, while low protein content existed different pattern from other malting characteristics. Among the favorable hordein patterns, some patterns showed the outstanding character in both seed sets, in the other meanings, under different environmental planting conditions. Moreover, two sets of protein patterns of B-hordein and C-hordein showed favorable malting quality characters, in which the same bands of each hordein type were observed in both sets. The B-hordein were group having band number 32, 38, 44 and 46 and group having band number 36, 37, 38, 41, 43, 44, 46, 53, 56 and 57, while C-hordein were group having band number 7, 8, 12, 20, 21 and 23 and group having band number 5, 6, 18, 21, 24, 25 and 27.
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