Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP
Kim, T.J.;Kim, M.H.;Na, Y.R.;Lee, J.I.(Chonnam National University, Gwangju, Republic of Korea)E-mail:jaeil@chonnam.ac.kr | Jung, S.C.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn Ⅱ.
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