Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP

Kim, T.J.;Kim, M.H.;Na, Y.R.;Lee, J.I.E-mail:jaeil@chonnam.ac.kr; Jung, S.C.

Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP

Kim, T.J.;Kim, M.H.;Na, Y.R.;Lee, J.I.(Chonnam National University, Gwangju, Republic of Korea)E-mail:jaeil@chonnam.ac.kr | Jung, S.C.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)

In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn Ⅱ.

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Ключевые слова АГРОВОК

brachyspira hyodysenteriae nitrogen oxides nox oxyde d'azote 돼지

Библиографическая информация

Опубликовано в

Korean Journal of Veterinary Research

Том 44 Выпуск 4 ISSN 1225-0198

Нумерация страниц

pp. 533-537

Другие темы

Nadh oxidase(nox) 유전자; Intestinal spirochetes; Pcr-rflp; Oxidos de nitrogeno; Brachyspira pilosicoli; 국내분리주의

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Примечание

Summary(En)

3 tables

3ill., 16 ref.

Переведенный заголовок

NADH oxidase(nox) 유전자의 PCR-RFLP를 이용한 돼지 intestinal spirochetes 국내분리주의 동정

Тип

AGRIS — международная информационная система по сельскохозяйственным наукам и технологиям

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Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP

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