Screening of lactic acid bacteria from Apis mellifera L. and Trigona spp. against Paenibacillus larvae (White) causing American foul brood disease of honeybees
2014
Montecillo, A.D. | Sabino, N.G. | Fajardo, A.Jr. | Cervancia, C.R. | Aborot, Z.A. | Perdigon, K.M.D. | Lantican, N.B.
A total of 28 putative lactic acid bacteria were isolated consisting of 23 isolates from the hindguts of Apis mellifera and 5 from Trigona spp.. The isolates were screened for inhibitory activity against the bee pathogen Paenibacillus larvae (white) using stab-on agar assay. Nine isolates scored positive (with 2 mm zone of inhibition on stab-on assay) for inhibition of the pathogen. Two of these isolates exhibited good antagonistic activity. These were identified as Lactobacillus plantarum based on carbohydrate fermentation patterns and through 16S rRNA gene sequencing.
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