Screening of lactic acid bacteria from Apis mellifera L. and Trigona spp. against Paenibacillus larvae (White) causing American foul brood disease of honeybees
2014
Montecillo, A.D. | Sabino, N.G. | Fajardo, A.Jr. | Cervancia, C.R. | Aborot, Z.A. | Perdigon, K.M.D. | Lantican, N.B.
A total of 28 putative lactic acid bacteria were isolated consisting of 23 isolates from the hindguts of Apis mellifera and 5 from Trigona spp.. The isolates were screened for inhibitory activity against the bee pathogen Paenibacillus larvae (white) using stab-on agar assay. Nine isolates scored positive (with 2 mm zone of inhibition on stab-on assay) for inhibition of the pathogen. Two of these isolates exhibited good antagonistic activity. These were identified as Lactobacillus plantarum based on carbohydrate fermentation patterns and through 16S rRNA gene sequencing.
显示更多 [+] 显示较少 [-]