Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

Miyoshi, Tatsuo; Iwatsuki, Teruki; Naganuma, Takeshi

Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

2005

Miyoshi, Tatsuo | Iwatsuki, Teruki | Naganuma, Takeshi

A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-[micro]m-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-[micro]m-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-[micro]m-pore-size filters were largely affiliated with BETAPROTEOBACTERIA:

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Palabras clave de AGROVOC

analysis bacteria classification dna dna filters filtration fresh water genes genes genetics groundwater japan microbiology phylogeny polymerase chain reaction restriction fragment length polymorphism ribosomal rna rna rrna sequence analysis taxonomy

Información bibliográfica

Publicado en

Applied and environmental microbiology

Volumen 71 Edición 2 Paginación 1084 - 1088 ISSN 0099-2240

Otras materias

Operational taxonomic units; Molecular sequence data; Nucleotide sequences; Ribosomal; Molecular sequence data; Community ecology; Water resources and management; Isolation purification; Micropore filters; 16s; Ribosomal; Beta-proteobacteria

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Tipo

Journal Article; Text

En AGRIS desde: 2024-02-29

Formato: MODS

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Enlaces

DOI http://dx.doi.org/10.1128/AEM.71.2.1084-1088.2005

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