Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

Miyoshi, Tatsuo; Iwatsuki, Teruki; Naganuma, Takeshi

Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

2005

Miyoshi, Tatsuo | Iwatsuki, Teruki | Naganuma, Takeshi

A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-[micro]m-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-[micro]m-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-[micro]m-pore-size filters were largely affiliated with BETAPROTEOBACTERIA:

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Ключевые слова АГРОВОК

analysis bacteria classification dna dna filters filtration fresh water genes genes genetics groundwater japan microbiology phylogeny polymerase chain reaction restriction fragment length polymorphism ribosomal rna rna rrna sequence analysis taxonomy

Библиографическая информация

Опубликовано в

Applied and environmental microbiology

Том 71 Выпуск 2 Нумерация страниц 1084 - 1088 ISSN 0099-2240

Другие темы

Operational taxonomic units; Molecular sequence data; Nucleotide sequences; Ribosomal; Molecular sequence data; Community ecology; Water resources and management; Isolation purification; Micropore filters; 16s; Ribosomal; Beta-proteobacteria

Язык

Английский

Лицензия

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Тип

Journal Article; Text

В АГРИСе с: 2024-02-29

Формат: MODS

Поставщик данных

Эту запись предоставил National Agricultural Library

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Ссылки

DOI http://dx.doi.org/10.1128/AEM.71.2.1084-1088.2005

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