Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system
2022
Yu Liu | Yinge Jin | Tao Chen | Yunqin Wu | Xiaohua Peng | Wansheng Li | Shu Wei | Min Chen | Qingjian Zou | Suqin Guo | Jucai Xu | Chengcheng Tang | Xiaoqing Zhou
The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex.
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