Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system

Yu Liu; Yinge Jin; Tao Chen; Yunqin Wu; Xiaohua Peng; Wansheng Li; Shu Wei; Min Chen; Qingjian Zou; Suqin Guo; Jucai Xu; Chengcheng Tang; Xiaoqing Zhou

Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system

2022

The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex.

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