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Haemophilus paragallinarum haemagglutinin: Role in adhesion, serotyping and pathogenicity
2008
Barnard, T.G.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Van Heerden, E.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Bragg, R.R.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Albertyn, J.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology)
It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pi and molecular mass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca²+ and Mn²+ ions.
Mostrar más [+] Menos [-]The testing and modification of a commercially available transport medium for the transportation of pure cultures of Haemophilus paragallinarum for serotyping
2004
Bragg, R.R. | Jansen van Rensburg, P. | Van Heerden, E. | Albertyn, J. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
Virulence of South African isolates of Haemophilus paragallinarum. Part 3: experimentally produced NAD-independent isolate
2002
Taole, M. | Albertyn, J. | Van Heerden, E. | Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology Dept.)
Virulence of South African isolates of Haemophilus paragallinarum. Part 1: NAD-dependent field isolates
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
Isolation of serovar C-3 Haemophilus paragallinarum from Zimbabwe: A further indication of the need for the production of vaccines against infectious coryza containing local isolates of H. paragallinarum
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
Changes in the incidences of the different serovars of Haemophilus paragallinarum in South Africa: a possible explanation for vaccination failures
1996
Bragg, R.R. | Coetzee, L. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Poultry Diseases) | Verschoor, J.A.
Limitation of the spread and impact of infectious coryza through the use of a continuous disinfection programme
2004
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
Virulence of South African isolates of Haemophilus paragallinarum. Part 2: Naturally occuring NAD-independent field isolates
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
Confirmation that PCR can be used to identify NAD-dependent and NAD-independent Haemophilus paragallinarum isolates
1999
Miflin, J.K. (Animal Research Inst., Moorooka (Australia)) | Chen, X. | Bragg, R.R. | Welgemoed, J.M. | Greyling, J.M. | Horner, R.F. | Blackall, P.J.
Genetic organisation of the capsule transport gene region from Haemophilus paragallinarum
2004
De Smidt, O. | Albertyn, J. | Bragg, R.R. | Van Heerden, E. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
