Refinar búsqueda
Resultados 1-2 de 2
Hemagglutination and hemagglutination inhibition of turkey red blood cells with Mycoplasma hyopneumoniae.
1989
Young T.F. | Erickson B.Z. | Ross R.F. | Wannemuehler Y.
The ability of Mycoplasma hyopneumoniae to agglutinate RBC was evaluated to develop an in vitro cytadsorption assay. Using swine RBC in a microtitration hemagglutination test, no agglutination or partial agglutination was detected. Comparison of RBC from various other species indicated that improved hemagglutination was obtained with RBC from turkeys. This hemagglutination was detected only when mycoplasma cells used in the assay had been frozen and thawed, heated at 50 C for 30 minutes, or treated with trypsin. Treatment of RBC with trypsin or neuraminidase enhanced hemagglutination. Possible surface lectin activity in M hyopneumoniae was evaluated by use of carbohydrates in a blocking assay; hemagglutination was not inhibited by any of 13 carbohydrates evaluated. Mycoplasma hyopneumoniae convalescent porcine serum and monoclonal antibodies against 2 M hyopneumoniae immunogens of molecular weights of 64,000 and 41,000 inhibited hemagglutination.
Mostrar más [+] Menos [-]Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against sendai virus, mouse hepatitis virus and mycoplasma pulmonis in mice and rats
1989
Chung, Y.Y. (Korea Air and Correspondence Univ., Seoul (Korea R.). Dept. of Agriculture) | Lee, H.C. | Lee, E. | Yoo, B.S. (Youngnam Univ., Kyongsan (Korea R.). Coll. of Agriculture and Animal Science)
This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine (mice and rats) antibodies against hemagglutinating virus of Japan (HVJ), mouse hepatitis virus (MHV) and Mycoplasma pulmonis (Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immuno-sorbent assay (ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats
Mostrar más [+] Menos [-]