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Development of enzyme linked immunosorbent assay for seromonitoring against hydropericardium syndrome (ELISA) virus
2003
Manzoor, R. | Kamal, T. | Khawaja, D.A. | Ismail, M.A. | Gill, Z.J. (Veterinary Research Inst., Lahore (Pakistan))
In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).
Mostrar más [+] Menos [-]Evaluation of coproantigen diagnosis for natural Echinococcus multilocularis infection in red foxes [Vulpes vulpes]
1999
Morishima, Y. (Hokkaido Univ., Sapporo (Japan)) | Tsukada, H. | Nonaka, N. | Oku, Y. | Kamiya, M.
The validity of a coproantigen ELISA for Echinococcus multilocularis was evaluated by comparison of three diagnostic methods; autopsy, egg examination and the ELISA. Of 71 foxes, 39 were found to be infected with the cestode at autopsy. The overall mean of worm burdens was 3,451, but the number varied (1-34,522). The ELISA could detect 94.9% (37/39) of the worm positives and there were no false-positives. Two false-negatives were infected with 1 and 4 cestodes, whereas 3 cases with similar worm burdens (2, 4 and 6 worms) were diagnosed as positives. This indicates the detection limit of the assay may be equivalent to less than 10 (in the worm burden). On the other hand, egg examination showed low sensitivity (43.6%, 17/39). These results suggest the ELISA has a potential to replace for the conventional methods
Mostrar más [+] Menos [-]Bovine leukemia virus infection in Taiwan: Evalution of the enzyme linked immunosorbent and agar gel immunodiffusion test
1991
Wang, C.T. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Use of biotinylated antibody for the assay of Hanganutziu-Deicher antibodies and antigens in fluids and tissues from cancer patients
1989
Gathuru, J.K. (Yokohama Univ. (Japan). Faculty of Engineering) | Higashi, H. | Kato, S. | Usuba, O. | Naiki, M.
Evaluation of isometamidium levels in the serum of sheep and goats after prophylactic treatment against trypanosomosis
2004
Wesongah, J.O. | Murilla, G.A. | Kibugu, J.K. (Kenya Trypanosomiasis Research Inst., Kikuyu (Kenya)) | Jones, T.W.
Evaluation of an indirect enzyme-linked immunosorbent assay for the detection of feline lentivirus-reactive antibodies in wild felids, employing a puma lentivirus-derived synthetic peptide antigen
2003
Van Vuuren, M. | Stylianides, E. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Kania, S.A. | Zuckerman, E.E. | Hardy, W.D.
Development of solid phase antigen for indirect ELISA for the detection of specific antibody responses to infection with Newcastle disease virus
2002
Sahle, M. (National Animal Health Research Center, Sebeta (Ethiopia)) | Burgess, G.W.
The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus
1999
Du Plessis, D.H. | Van Wyngaardt, W. | Romito, M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Du Plessis, M. | Maree, S.
Detection of bovine-virus-diarrhoea-virus antibodies in cattle with an enzyme-linked immunosorbent assay
1995
Muvavarirwa, P. | Mudenge, D. | Moyo, D. | Javangwe, S. (Zimbabwe Univ., Harare (Zimbabwe). Dept. of Paraclinical Veterinary Studies)
The use of sucrose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test
1995
Paweska, J.T. | Barnard, B.J.H. | Williams, R. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)
