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Development of enzyme linked immunosorbent assay for seromonitoring against hydropericardium syndrome (ELISA) virus
2003
Manzoor, R. | Kamal, T. | Khawaja, D.A. | Ismail, M.A. | Gill, Z.J. (Veterinary Research Inst., Lahore (Pakistan))
In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).
Mostrar más [+] Menos [-]Evaluation of an indirect enzyme-linked immunosorbent assay for the detection of feline lentivirus-reactive antibodies in wild felids, employing a puma lentivirus-derived synthetic peptide antigen
2003
Van Vuuren, M. | Stylianides, E. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Kania, S.A. | Zuckerman, E.E. | Hardy, W.D.
Detection of antibodies to the Ehrlichia ruminantium MAP1-B antigen in goat sera from three communal land areas of Zimbabawe by an indirect enzyme-linked immunosorbent assay
2003
Kakono, O. | Hove, T. (Veterinary Science Faculty, Harare (Zimbabwe)) | Geysen, D. | Mahan, S.
Indirect enzyme-linked immunosorbent assay for the detection of antibody against Rift Valley fever virus in domestic and wild ruminant sera
2003
Paweska, J.T. | Smith, S.J. | Wright, I.M. | Williams, R. | Cohen, A.S. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Van Dijk, A.A. | Grobbelaar, A.A. | Croft, J.E. | Swanepoel, R. | Gerdes, G.H.
Comparison of complement fixation test, immunoblotting, indirect ELISA, and competitive ELISA for detecting antibodies to Mycoplasma mycoides subspecies mycoides small colony (SC) in naturally infected cattle from the 1995 outbreak in Botswana
2003
Marobela-Raborokgwe, C. (National Veterinary Laboratory, Gaborone (Botswana)) | Nicholas, R. | Ayling, R. | Bashiruddin, J.B.
