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Stimulatory effects of Bordetella bronchiseptica antigen on bone marrow cells and immune memory responses
2014
Yim, S.H., Jeju National University, Jeju, Republic of Korea | Joo, H.G., Jeju National University, Jeju, Republic of Korea
Bone marrow is a hematological and immunological organ that provides multiple immune cells, including B lymphocytes, and thus plays a critical role in the efficacy of vaccine. We previously demonstrated that Bordetella (B.) bronchiseptica antigen has high immunogenicity in spleen cells, a peripheral immune organ. In this study, we investigated the immunogenicity of B. bronchiseptica antigen in bone marrow cells, a central immune organ. B. bronchiseptica antigen increased the cellular activity of bone marrow cells and significantly enhanced the production of nitric oxide, IL-6, and TNF-¥�. Bone marrow cells primed with B. bronchiseptica antigen in vivo were harvested and stimulated with the same antigen in vitro. The stimulation of B. bronchiseptica antigen significantly increased the cellular activity and proliferation rate of the primed cells. B. bronchiseptica antigen also greatly induced the production of antigen-specific antibody in the primed cells. Taken together, the present study demonstrated that B. bronchiseptica antigen can stimulate bone marrow cells, a central immune organ, and recall the immune response of the primed bone marrow cells.
Mostrar más [+] Menos [-]Protective effects and immunogenicity of Salmonella Enteritidis killed vaccine strains selected from virulent Salmonella Enteritidis isolates
2011
Kang, Z.W., Kangwon National University, Chuncheon, Republic of Korea | Won, H.K., ChoongAng Vaccine Laboratories, Daejeon, Republic of Korea | Kim, E.H., ChoongAng Vaccine Laboratories, Daejeon, Republic of Korea | Noh, Y.H., ChoongAng Vaccine Laboratories, Daejeon, Republic of Korea | Choi, H.W., ChoongAng Vaccine Laboratories, Daejeon, Republic of Korea | Hahn, T.W., Kangwon National University, Chuncheon, Republic of Korea
Salmonella Enteritidis (SE) has been a major causative agent of food-borne human disease due to consumption of contaminated eggs and poultry meat. To prevent SE infection in poultry, and therefore minimize human infections, vaccination with either killed or live SE vaccine is suggested. We evaluated a newly developed killed bacterin using a representative SE isolate in Korea. Among pool of SE isolates, two highly virulent isolates (the one isolate from chicken, the other from human) were selected by measuring mortality in mouse and chickens administered. The chickens were injected intramuscularly with killed vaccine and were challenged with highly virulent SE strain 3 week after vaccination. The recovered colony count (cfu/g) of spleen and cecal content in the vaccinated groups was reduced compared with those of the unvaccinated control group. The antibody level in the vaccinated groups was higher at 3 week post vaccination. These results indicate that vaccination with killed vaccine was effective in preventing the infection of virulent SE. Further study for a large number of layers should be needed for the effect of egg production, SE shedding in feces, persistence of antibody level.
Mostrar más [+] Menos [-]Evaluation on efficacy of β-hemolytic Streptococcus iniae vaccine on olive flounder, Paralichthys olivaceus
2007
Moon, J.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: moonjs@nvrqs.go.kr | Jang, H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Joh, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, M.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Son, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of β-hemolytic Streptococcus (S.) iniae vaccine on olive flounder.
Mostrar más [+] Menos [-]Comparison of immune responses of dogs and guinea pigs inoculated with inactivated canine coronavirus vaccines
2005
An, D.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: kimbh@nvrqs.go.kr | Jung, B.Y. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Yi, C.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jeon, W.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Lee, P.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Chung, G.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
Canine coronavirus (CCV) causes a mild gastroenteritis in dogs. The virus is highly contagious. Although the virus was isolated more than thirty years ago, canine coronavirus infection continues to be a widespread problem. Mixed infections with both CCV and canine parvovirus (CPV) are common. Four kinds of commercial killed CCV vaccines are available in Korea. All the commercial vaccines should pass the National Assay for Veterinary Biologicals prior to release. For the potency test of CCV vaccine, it is necessary to use CCV antibody free dogs. The test requires not only kennels but high cost. To develop easy, efficient and economic potency test method for killed CCV vaccine using laboratory animals, a series of experiments with rabbits and guinea pigs were carried out in this study.
Mostrar más [+] Menos [-]Effect of a trivalent (FPV, FHV, FCV) inactivated vaccine in kittens
2005
Lee, S.M. (Woosung Co., Ltd., Daejeon, Republic of Korea), E-mail: mini7226@hanmail.net | Yoon, I.J. (ChoongAng Vaccine Laboratory, Daejeon, Republic of Korea) | Lee, K.J. (Chungnam Livestock and Veterinary Service Institute, Gongju, Republic of Korea) | Lee, K.Y. (Kagawa University, Kagawa, Japan) | Kim, M.K. (Chungnam National University, Daejeon, Republic of Korea) | Choi, H.W. (ChoongAng Vaccine Laboratory, Daejeon, Republic of Korea)
This study tested the effect of a trivalent (feline panleukopenia; FPV, feline viral rhinotracheitis; FHV, feline calicivirus infection; FCV) inactivated vaccine in cats. The vaccine was tested for the safety in guinea pigs, mice and cats. Also, it was tested for the efficacy in cats. The vaccine was inoculated to cats at 7~9 and 10~12 weeks of age (conventional schedule) and the serological response to vaccination was assessed and was compared to the unvaccinated group. All cats were bled by jugular venipuncture for FPV, FHV and FCV specific serological test (virus neutralizing antibody, VN) at 7~9, 10~12 and 13~15 weeks.
Mostrar más [+] Menos [-]Immunostimulatory effects of BCG-CWS on the proliferation and viability of mouse spleen cells
2012
Lee, C.W., Jeju National University, Jeju, Republic of Korea | Ko, E.J., Jeju National University, Jeju, Republic of Korea | Joo, H.G., Jeju National University, Jeju, Republic of Korea
Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been tried to use for immunotherapy. The CWS of Mycobacterium bovis bacillus Calmette-Guerin (BCG-CWS) was studied as an universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccine. Although a variety of biological activities of BCG-CWS have been studied, the effects of BCG-CWS on spleen cells are not fully elucidated. Using MTT assay and trypan blue exclusion test, we found that BCG-CWS significantly enhanced the viability and proliferation of cells. Multiple clusters, indicating proliferation, were observed in BCG-CWS-treated spleen cells and surface marker staining assay revealed that BCG-CWS promoted the proliferation of CD19+ B lymphocyte rather than CD4+ or CD8+ T lymphocyte. In addition, BCG-CWS up-regulated the expression of anti-apoptotic molecules such as bcl-2, bcl-xL. BCG-CWS increased the surface expression of CD25 and CD69 as well as IL-2 production of spleen cells, suggesting increased activation. Furthermore, BCG-CWS enhanced the antigen-specific cell proliferation and interferon-gamma production of spleen cells. Taken together, these results demonstrate the immunostimulatory effects of BCG-CWS on spleen cells via multiple mechanisms, providing valuable information to broaden the use of BCG-CWS in clinical and research settings.
Mostrar más [+] Menos [-]Evaluation of stability and potency of live attenuated rinderpest vaccine of lapinized-avianized tissue culture strain origin for the establishment of expiration period
2009
Yeh, J.Y., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, H.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Nah, J.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Park, J.Y., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lee, J.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Seo, H.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kweon, C.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Cho, I.S., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Moon, J.S., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea
In this study, we evaluated the stability and potency of live attenuated rinderpest vaccines of lapinized-avianized tissue culture strain origin, which had been produced annually from 2005 to 2008. When immune responses to the vaccines were evaluated using two Holstein calves weighing 100~150 kg, neutralizinng antibody titer of 1 : 16 was induced at 21 days post vaccination. When calves were also inoculated with vaccines lots that had been stored for 39 months at 4℃, same level of antibody titer was observed. Using the virus titer test, we found that all batches of the vaccine that had been kept for 3, 10, 15, 22, 27, 34, 39, and 45 months showed no significant loss of titers, and fulfilled the requirement necessary (greater-than or equal to 3 logTCID∧50) to be used as the national rinderpest vaccine reserve in Korea. In this study, we demonstrated that stability and potency of the rinderpest vaccines were maintained over three years when kept at 4℃ storage. This indicates that it maybe feasible to extend the expiration period of this vaccine from one year to three years.
Mostrar más [+] Menos [-]Comparison of tissue tropism of Newcastle disease vaccine viruses by Immunohistochemistry techniques
Kim, M.J.;Kwon, Y.K.;Seong, H.W.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kang, S.Y.;Mo, I.P.(Chungbuk National University, Cheongju, Republic of Korea)E-mail:moip@cbu.ac.kr
Mean death time of inoculated embryonated egg is one of the methods to determine the virulence of the Newcastle disease viruses (NDV). Evaluation of tissue tropism of NDV in the host has been proposed as an another way to determine the pathogenicity of NDV based on the principal site of viral replication. To evaluate the tissue tropism among NDV, an immunohistochemistry(IHC) technique using monoclonal antibody was applied in one-day-old SPF chickens inoculated with different ND vaccine strains such as Ulster 2C, VG/GA and B1 viruses by eye drop instillation.
Mostrar más [+] Menos [-]Studies on development of Escherichia coli subunit vaccine against calf diarrhea, 2; immunogenicity and protective efficacy of Escherichia coli K99 and F41 pilus vaccines in experimental animals
1986
Kim, J.M. | Yoon, Y.D. | Park, J.M. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Inst.) | Kim, B.H. (Kyongbuk National Univ., Taegu (Korea R.). Dept. of Veterinary Medicine)
The oil emulsion and alhydrogel pilli vaccines were prepared from a strain (O9: K35, K99, F41) of enterotoxigenic Escherichia coli isolated from calves with diarrhea and their immunogenicity was tested in guinea-pigs, pregnant goats and cows. Serum antibody responses to K99 and F41 antigens in guinea-pigs given experimental oil and gel vaccines peaked at 4 and 6 weeks after vaccinations. At that time, the mean hemagglutination inhibition titers to K99 and F41 antigens in guinea pigs given oil vaccine were 1:25 and 1:1,218 and those given gel vaccine were 1:54 and 1:724 respectively
Mostrar más [+] Menos [-]Studies on the antigenicity and immunogenicity of Newcastle disease virus inactivated with binary ethylenimine
1985
Park, B.K. | Jeon, Y.S. | Lee, Y.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Veteinary Medicine) | Rhee, Y.O. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Inst.)
Effects of binary ethylenimine (BEI) treatment on the inactivation of infectivity and hemagglutinin of Newcastle disease virus (NDV) were studied in comparison with those of formalin treatment. Immune responses of chickens vaccinated with BEI- inactivated NDV vaccines were also investigated. Complete loss of infectivity of NDV (BI) was observed at 3, 7, and 24 hrs after the treatment at 37 deg C with BEI concentrations of 0.01M, 0.005M and 0.001M, respectively
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