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Systemic Aspergillosis associated with bovine viral diarrhea virus infection in Korean native calves
2005
Jean, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: jean@nvrqs.go.kr | Kim, J.H. (Cheju National University, Jeju, Republic of Korea) | Kim, D.Y. (Seoul National University, Seoul, Republic of Korea) | Jeong, S.W. (Konkuk University, Seoul, Republic of Korea) | Cho, D.Y. (Louisiana State University, Baton Rouge, LA, USA) | Moon, O.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Systemic aspergillosis were outbroken of 17 Korean native calves at livestock farm in Kyunggi province. Two 3 months old calves were died after having diarrhea, coughing, dyspnea, nasal discharge, and abdominal breathing. These calves were diagnosed of aspergillosis as multifocal to coalescing pyogranulomatous inflammation with intralesional aspergillus infiltration at liver, kidney, spleen, heart, lymph nodes, lungs, intestine and cerebrum. Aspergillus fumigatus was cultured from the affected tissues. And the small intestine was positive against bovine viral diarrhea (BVD) virus on FA test. We considered that the secondary fungal infections killed the calves after BVD virus infection primarily.
Mostrar más [+] Menos [-]Detection of bovine viral diarrhea virus by In situ hybridization
1999
Park, N.Y. | Hong, K.K. | Chung, C.Y. | Cho, K.O. | Lee, B.J. | Park, Y.S. | Park, H.S. (Chonnam National Univerisyt, Kwangju (Korea Republic). College of Veterinary Medicine) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
Detection and distribution of bovine biral diarrhea virus(BVDV) was studied in formalin-fixed, paraffin-embedded tissues from two naturally infected cattle by in situ hybridization with a non-radioactive biotinylated probe. A 600 base pair cDNA probe from BVDV B-25 strain ws used for probe. The whole procedure of ISH to diagnose was carried out within 1~2 hours in MicroprobeTM capillary action system. The biotin-labelled probe was demonstrated after hybridization under standard conditions by the application of streptoavidin and biotinylated alkaline phosphatase. Alkaline phosphatase was visualized using a fast red TR/naphthol phosphatase and the sections were counterstained with hematoxylin. We have obtained the result of positive reactions in digestive tract(sm1.all intestine and colon) and epidermis of tongue in the state of the intract tissues. The result suggested that in situ hybridization method can be considered as a useful diagnostic technique for detection of specific nucleic acid sequences of BVDV.
Mostrar más [+] Menos [-]Differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis by polymerase chain reaction
1998
Seong, H.W. (National Veterinary Research Institute, RDA, Anyang (Korea Republic)) | Kim, S.J. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
Effects of ibaraki virus on viability of preimplantation mouse embryos
1989
Kim, Y.J. | Jo, C.H. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine)
To study the effects of ibaraki virus on preimplantation mouse embryos collected from prepubertal ICR and BALB/cByJ mice (30-40 days old) by superovulation, zona pellucida-intact (ZPI) or free (ZPF) embryos (n=774) of 4- to 8-cell and morulae were exposed to 10** (5.8) TCID50 of the viurs up to 96 hours. The embryos were examined morphologically by observing the degeneration and hatching rates, and virologically and immunologically by determining the presence of infection with the virus, in addition, the effect of washing the embryos to remove virus possibly attached to was also investigated. The ZPI 4- to 8-cell embryos and morulae exposed to the virus showed considerably higher degeneration rate than those not exposed, for 96, and for 72 to 96 hours, respectively (p0.01). The ZPF 4- to 8-cell embryos and morulae exposed to the virus showed considerably higher degeneration rates than those not exposed, throughout the whole culture hours in vitro (p0.01). The ZPI 4- to 8-cell embryos and morulae not exposed to the virus showed considerably higher rates of hatched blastocyst than those exposed (p0.01). The virus infection rates of the ZPF 4- to 8-cell embryos and morulae were significantly higher than those of the ZPI embryos according to cell culture system. The viral antigen was detected exclusively on the zona pellucida of ZPF embryos by the immunofluorescent assay. In the ZPI embryos exposed to ibaraki virus, the virus was detected in the two times-washing groups, but not in the ten times-washing groups. The results indicated that zona pellucida of murine embryos would provide an effective protection and that ten times-washing of the ZPI embryos previously exposed to the virus was effective to remove virus from the embryos
Mostrar más [+] Menos [-]Light and electron microscopy of the kidney tissues on viral haemorrhagic disease in rabbits
1989
Kim, J.H. | Chung, C.Y. | Park, N.Y. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Veterinary Medicine)
In order to observe the pathological changes of kidney in rabbits infected with the viral haemorrhagic disease, the kidney tissues from the 91 rabbits infected with the viral haemorrhagic disease were examined by light and electron microscopy. On light microscopic observation, the kidney lesions were identified as haemorrhagic glomerular necrosis (33.0 %), membranous glomerulonephritis (20.9 %), thrombotic glomerulopathy (19.8 %) membranoproliferative glomerulonephritis (8.8 %), mesangial proliferative glomerulonephritis (8.8 %) ischemic acute tubular necrosis (7.7 %), and acute serous glomerulitis (6.6 %). On electron microscopic observation, cytoplasmic degeneration of mesangial cells, and irregular thickening of basement membranes with electron dense granular materials were observed. In podocytes swelling of mitochondria, dilatation of endoplasmic reticulum and extensive fusion of foot processes were also observed. Nonenveloped round icosahedral picornaviral particles with a diameter of 28-33nm were detected in the cytoplasm of degenerative endothelial cells, polymorphonuclear leucocytes, and monocytes
Mostrar más [+] Menos [-]Serological survey of horses in Korea for evidence of Getah virus infection
1986
Rhee, Y.O. | Heo, Y. | Kim, Y.H. | Sul, D.S. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Inst.)
The prevalence of antibody to Getah virus was serologically followed among those horses in Korea. Blood samples were taken from 575 horses in Feb and 462 horses in Sep. 1985. Overall percentage of horses with seropositive to Getah virus was 37% in Feb and 47% in Sep. The data suggest that the Getah virus infection was endemic in Korea and the increase in prevalence may be associated with the seasonal activity of arthropod vectors. This entails preventive precautions of the horses against this viral infection before the entry into Korea
Mostrar más [+] Menos [-]An epidemiological survey on seroprevalence of vector mediated virus infection in cattle bred in a Japanese remote island, Okinawa
1998
Takeo, S. | Masaaki H. | Sakae, A. | Keiichi, F. (Nihon University, Fujisawa (Japan). Department of Preventive Veterinary Medicine) | Fumio, H.I. (Saopaulo University, Saopaulo (Brazil). Department of Veterinary Preventive Medicine) | Lee, W.C. | Lee, J.B. (Konkuk University, Seoul (Korea Republic). Department of Veterinary Public Health)
Studies on the cloning gp50 and gp63 genes of Pseudorabies virus (Shope strain)
1991
Kweon, C.H. | An, S.H. | Song, J.Y. | Kim, B.H. | Lee, J.B. | Lee, J.C. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Maeda, S. (Univ. of California, Davis (USA))
Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against sendai virus, mouse hepatitis virus and mycoplasma pulmonis in mice and rats
1989
Chung, Y.Y. (Korea Air and Correspondence Univ., Seoul (Korea R.). Dept. of Agriculture) | Lee, H.C. | Lee, E. | Yoo, B.S. (Youngnam Univ., Kyongsan (Korea R.). Coll. of Agriculture and Animal Science)
This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine (mice and rats) antibodies against hemagglutinating virus of Japan (HVJ), mouse hepatitis virus (MHV) and Mycoplasma pulmonis (Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immuno-sorbent assay (ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats
Mostrar más [+] Menos [-]RT-PCR and nested PCR amplification of the PRRSV genes from boar semen for the rapid and sensitive differential diagnosis
1998
Lyoo, Y.S. (Konkuk University, Seoul (Korea Republic). Veterinary Medicine) | Park, C.K. | Lee, C.H. (National Veterinary Research Institute, Anyang (Korea Republic))