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Investigation of The Effects of Some Intrafollicular Growth Factors (GDF-9, GATA-4, GATA-6, IGF-I, IGF-II) on Etiopathogenesis of Cystic Follicular Ovarian Degenerations in Cows Texto completo
2020
Polat, İbrahim Mert | Vural, Mehmet Rıfat
The aim of the presented study was to investigate the efficacy of intrafollicular GDF-9, GATA-4, GATA-6, IGF-I and IGF-II levels on etiopathogenesis of follicular cystic degenerations in dairy cows in accordance with some puerperal physiology parameters. After calving in the farm, all the cows were screened for the preovulatory and cystic follicles via ultrasonography with 5 MHz transrectal linear probe for the evaluation of ovarian activity at the days of postpartum 14, 24, 34, 44 and 55. Preovulatory and cystic follicular fluids were collected by aspiration via transvaginal ovum pick up method. Furthermore, uterine involution and vaginal discharges were evaluated by clinical examinations. Intrafollicular expression of GDF-9, GATA-4 and GATA-6 proteins were estimated by Western-blot assay and IGF-I levels were determined by ELISA. In cyst group, densitometric evaluation revealed that the expression of intrafollicular GDF-9, GATA-4 and GATA-6 and IGF-I levels were significantly lower than the control group (p<0.01). According to ovarian examinations, in cyst group, it was observed that at the day of postpartum 14 and 24, the difference in follicular diameters were significant between groups (p<0.01) and the follicles moved directly to cystic structure at the days of postpartum 24 or 34, is originated from same follicles at the day of postpartum 14. Involution process was observed to be slightly lower in cyst group, but, it was determined that there was no difference in uterine involutions among the study groups (p>0.05). In follicular cyst group, especially at the days of postpartum 24 and 34, the observation of severe mucotic vaginal discharges was found noteworthy. As a result, dairy cows presenting cystic ovaries, have lower intrafollicular GDF-9, GATA-4, GATA-6 and IGF-I levels than those of the preovulatory follicle group and also it has been suggested that, these proteins may play an active role in the etiopathogenesis of ovarian cysts and these findings may be associated with physiological parameters as well.
Mostrar más [+] Menos [-]EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILER Texto completo
2016
Suhair R. Al-Idreesi | Mahmoud Kweider | Mahmad M. Katranji
To determine the type of immune response to oocyst vaccine in broiler against coccidiosis,broilers were vaccinated with two doses of prepared oocyst protein from the local strain ofEmeria tenella parasite. The vaccine was applied on 3rdand 16th day of age subcutaneously ata dose (25 μg per chicken), vaccinated birds were challenged at 30 day of age. Blood sampleswere collected at (7th, 28thand 39th) day of age. The immunogenicity of vaccine was studiedby using SDS-PAGE and Western blot. Fourteen polypeptides had been estimated moreimmunogenic after probing with immunized chicken serum at 39th days of age, theirmolecular weight are (167.8, 114.5, 83.4, 78.2, 73.5, 53.3, 44.1, 38.8, 36.4, 28.2, 20.5, 18,14.9and 13.9) KD. In addition, the levels of ˠ -IFN and IL-4 were estimated in the serum ofimmunized chickens by using ELISA kits. The results demonstrated two types of immunity,cellular and humoral responses against E.tenella oocyst vaccine.
Mostrar más [+] Menos [-]Production of monoclonal antibody to 45 kDa somatic protein of Trichuris suis
Lee, J.K.(J.E.S., Seoul, Republic of Korea) | Kim, J.T.(Evergreen Animal Hospital, Seoul, Republic of Korea) | Seo, H.S.(Pfizer Animal Health Korea Ltd., Seoul, Republic of Korea) | Park, J.Y.(Bayer Korea Ltd., Seoul, Republic of Korea) | Youn, H.J.(Seoul National University, Seoul, Republic of Korea)
Trichuris suis does not excrete eggs during larval stage as well as in particular adult stage. It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis.
Mostrar más [+] Menos [-]Toxoplasma gondii: II. Caracterização antigênica de taquizoítos de oito amostras Texto completo
1998
Regina Mitsuka | Italmar Teodorico Navarro | Andrea Cláudia Beckner da Silva | José Wander Breganó | Amauri Alfieri | José Vitor Jankevicius | Odilon Vidotto
Toxoplasma gondii: II. Caracterização antigênica de taquizoítos de oito amostras Texto completo
1998
Regina Mitsuka | Italmar Teodorico Navarro | Andrea Cláudia Beckner da Silva | José Wander Breganó | Amauri Alfieri | José Vitor Jankevicius | Odilon Vidotto
Oito amostras de T. gondii - LIV IV, LIV V e S 11 isoladas de suínos, RH e VPS de seres humanos, AS 28 de camundongo, HV III de cão e CN de gato - foram analisadas com o objetivo de verificar a existência de possíveis diferenças na resposta imune quando inoculadas em coelhos. Através da técnica de ELISA, não foram constatadas diferenças entre as oito amostras estudadas. Todas as amostras reagiram de forma semelhante com soros homólogos e heterólogos. A suspensão antigênica, constituída de extrato celular total, mostrou-se eficiente no ELISA teste indireto, já que os soros positivos reagiram fortemente e os soros negativos não apresentaram reação contra os antígenos testados. A análise das amostras, pela técnica de Western blot, revelou que os isolados de T. gondii compartilham vários antígenos com algumas variações. Dentre as bandas reconhecidas no Western blot, três foram comuns a todas as amostras: a p33 (33-37 kDa), p54 (52-55 kDa) e a p66 (66 kDa). A amostra HV III, isolada recentemente de um cão, foi a que mais diferiu no perfil antigênico. Essa amostra não apresentou três antígenos (50, 70 e 75 kDa) presentes nas demais amostras. Apenas dois antígenos, um de 62 kDa da CN e outro de 67 kDa da LIV IV, foram amostra-específicos.
Mostrar más [+] Menos [-]Toxoplasma gondii: II. Caracterização antigênica de taquizoítos de oito amostras Texto completo
1998
MITSUKA, Regina(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva) | NAVARRO, Italmar Teodorico(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva) | BECKNER DA SILVA, Andrea Cláudia(Universidade Estadual de Maringá) | BREGANÓ, José Wander(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva) | ALFIERI, Amauri(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva) | JANKEVICIUS, José Vitor(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva) | VIDOTTO, Odilon(Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva)
Oito amostras de T. gondii <FONT FACE="Symbol">-</FONT> LIV IV, LIV V e S 11 isoladas de suínos, RH e VPS de seres humanos, AS 28 de camundongo, HV III de cão e CN de gato <FONT FACE="Symbol">-</FONT> foram analisadas com o objetivo de verificar a existência de possíveis diferenças na resposta imune quando inoculadas em coelhos. Através da técnica de ELISA, não foram constatadas diferenças entre as oito amostras estudadas. Todas as amostras reagiram de forma semelhante com soros homólogos e heterólogos. A suspensão antigênica, constituída de extrato celular total, mostrou-se eficiente no ELISA teste indireto, já que os soros positivos reagiram fortemente e os soros negativos não apresentaram reação contra os antígenos testados. A análise das amostras, pela técnica de Western blot, revelou que os isolados de T. gondii compartilham vários antígenos com algumas variações. Dentre as bandas reconhecidas no Western blot, três foram comuns a todas as amostras: a p33 (33-37 kDa), p54 (52-55 kDa) e a p66 (66 kDa). A amostra HV III, isolada recentemente de um cão, foi a que mais diferiu no perfil antigênico. Essa amostra não apresentou três antígenos (50, 70 e 75 kDa) presentes nas demais amostras. Apenas dois antígenos, um de 62 kDa da CN e outro de 67 kDa da LIV IV, foram amostra-específicos. | Eight Toxoplasma gondii strains were analyzed using ELISA and Western blot techniques, in order to demonstrate possible immunological differences. The analyzed strains were: LIV IV, LIV V and S 11 isolated from swine, RH and VPS from a human being, AS 28 from a wild mouse, HV III from a dog and CN from a cat. With the ELISA assay the eight strains showed similar reactivity with homologous and heterologous sera. The antigenic suspension, consisting of total cellular extract of tachyzoites, was effective in the indirect ELISA assay, with the positive sera reacting strongly and the negative not reacting with the antigens. The Western blot analysis showed that the T. gondii strains have similar antigenic profiles with a few variations. Three bands were observed in all strains: one of about 33 kDa (p33), another of 54 kDa (p54) and a third one of 66 kDa (p66). The HV III strain, isolated from a dog, did not show three antigens (50, 70 and 75 kDa) that were present in the others. However, this difference was not detected by the ELISA assay. Only two antigens (62 kDa of the CN and 67 kDa of the LIV IV) were strain-specific antigens.
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