Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes

Subotic, A.E-mail:heroina@ibiss.bg.ac.yu; Budimir, S.; Grubisic, D.; Momcilovic, I.

Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes

Subotic, A.(Institute for Biological Research)E-mail:[email protected] | Budimir, S.(Institute for Biological Research) | Grubisic, D.(Institute for Biological Research) | Momcilovic, I.(Institute for Biological Research)

Stable transformation and expression of transgenes was achieved in Centaurium erythraea using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog's half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction analysis.

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Mots clés AGROVOC

agrobacterium rhizogenes agronomic characters brotes clone clones cultivo in vitro culture in vitro fenotipos gene transfer genetic stability genetic vectors gentianaceae in vitro culture in vitro experimentation in vitro regeneration pcr phenotypes plasmide plasmidios plasmids pousse racine roots shoots transferencia de genes transgenic plants

Informations bibliographiques

Publié dans

Biologia Plantarum (Czech Republic)

Volume 47 Numéro 4 ISSN 0006-3134

Pagination

pp. 617-619

D'autres materias

Centaurium erythraea; Phenotype; Regeneration in vitro; Caracteristicas agronomicas; Vecteur genetique; Caractere agronomique; Vectores geneticos; Regeneracion in vitro; Transfert de gene; Plantas transgenicas; Stabilite genetique; Raices; Plante transgenique; Experimentacion in vitro; Experimentation in vitro; Estabilidad genetica

Langue

anglais

Note

Summary (En)

1 graph, 4 ill.

7 ref.

Type

Summary

Sur AGRIS depuis: 2005-05-15

Format: AGRIS AP

Fournisseur de données

Cette notice bibliographique a été fournie par Library of Antonin Svehla

Découvrez la collection de ce fournisseur de données dans AGRIS

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http://www.ueb.cas.cz/bp/bp.htm

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Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes

Mots clés AGROVOC

Informations bibliographiques