Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes

Subotic, A.E-mail:heroina@ibiss.bg.ac.yu; Budimir, S.; Grubisic, D.; Momcilovic, I.

Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes

Subotic, A.(Institute for Biological Research)E-mail:[email protected] | Budimir, S.(Institute for Biological Research) | Grubisic, D.(Institute for Biological Research) | Momcilovic, I.(Institute for Biological Research)

Stable transformation and expression of transgenes was achieved in Centaurium erythraea using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog's half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction analysis.

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AGROVOC关键词

agrobacterium rhizogenes agronomic characters brotes clone clones cultivo in vitro culture in vitro fenotipos gene transfer genetic stability genetic vectors gentianaceae in vitro culture in vitro experimentation in vitro regeneration pcr phenotypes plasmide plasmidios plasmids pousse racine roots shoots transferencia de genes transgenic plants

书目信息

发表于

Biologia Plantarum (Czech Republic)

卷 47 期 4 ISSN 0006-3134

页码

pp. 617-619

其它主题

Centaurium erythraea; Phenotype; Regeneration in vitro; Caracteristicas agronomicas; Vecteur genetique; Caractere agronomique; Vectores geneticos; Regeneracion in vitro; Transfert de gene; Plantas transgenicas; Stabilite genetique; Raices; Plante transgenique; Experimentacion in vitro; Experimentation in vitro; Estabilidad genetica

语言

英语

注释

Summary (En)

1 graph, 4 ill.

7 ref.

类型

Summary

自何时收录于AGRIS: 2005-05-15

格式: AGRIS AP

数据提供者

这条记录提供自 Library of Antonin Svehla