Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes
Subotic, A.(Institute for Biological Research)E-mail:[email protected] | Budimir, S.(Institute for Biological Research) | Grubisic, D.(Institute for Biological Research) | Momcilovic, I.(Institute for Biological Research)
Stable transformation and expression of transgenes was achieved in Centaurium erythraea using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog's half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction analysis.
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