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Over-Expression of Chlamydia Psittaci MOMP in Escherichia Coli and its Purification
2006
Ha, J.S. (Kyonggi University, Suwon, Republic of Korea) | Lee, D.B. (Kyonggi University, Suwon, Republic of Korea) | Han, S.H. (Kyonggi University, Suwon, Republic of Korea) | Lim, Y.K. (Cheju National University, Jeju, Republic of Korea) | Yoon, B.S. (Kyonggi University, Suwon, Republic of Korea), E-mail: [email protected]
Generally known psittacosis or ornithosis is a disease of birds caused by the bacterium Chlamydia psittaci. Humans are accidential hosts and are most commonly infected from avian sources. It raises hepatitis or neurosis. As major outer membrane protein (MOMP) of Chlamydia psittai has been known to play a role in the avoidance of host immune defenses, research on developing a Chlamydia vaccine has focused on the MOMP. In this study, the gene encoding the major outer membrane protein (MOMP) of the Chlamydia psittaci strain 6BC was cloned and expressed in Escherichia coli Strain M-15.
Afficher plus [+] Moins [-]Iridovirus Infection of Cultured Juvenile Flounder (Paralichthys Olivaceus) in Nursery
2006
Kim, T.J. (Chonnam National University, Gwangju, Republic of Korea) | Jang, E.J. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.S. (Jeonnam Biotechnology Research Center, Hwasun, Republic of Korea) | Lee, J.I. (Chonnam National University, Gwangju, Republic of Korea), E-mail: [email protected]
Iridovirus is an icosahedral cytoplasmic double-stranded DNA virus with a genome size of 170-200kb. Outbreaks of fish iridovirus infection are characterized by their wide geographic distribution and broad host spectrum, especially in water temperatures of 22-27℃ Recently, the causative agent of high mortalities in flounder (Paralichthys olivaceus) was identified as fish iridovirus in Korea. Iridoviral infection repeatedly occurs in the same area for long periods, suggesting the possibility of viral infection in nursery.
Afficher plus [+] Moins [-]Acupuncture Treatment of Torticollis in a Foal
2006
Kim, D.H. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected] | Liu, J.Z. (Chungnam National University, Daejeon, Republic of Korea) | Lee, J.Y. (Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan) | MacManus, Philip (Glenina Veterinary Clinic, Galway, Co. Galway, Ireland) | Jennings, Padraic (Glenina Veterinary Clinic, Galway, Co. Galway, Ireland) | Darcy, Karl (Glenina Veterinary Clinic, Galway, Co. Galway, Ireland) | Burke, Fiona (Glenina Veterinary Clinic, Galway, Co. Galway, Ireland) | A. Philip (Chungnam National University, Daejeon, Republic of Korea) | Rigers, M. (Teagasc Grange Research Centre, Dunsany, Co. Meath, Ireland)
A 6-month-old thoroughbred filly foal had torticollis and circled towards the right side. A local veterinarian treated her twice using dexamethasone for 1 week but there was little clinical improvement. Needles were inserted into Ting points of both hind limb and one ocular acupoint (shang jiao area from GB01). Injection acupuncture (dexamethasone, twice/week) was used at GV16, GB20, BL10 and LU07, for the neck and head. Acupoint GB34 was added to those points at session 4. In addition, a trigger point in the left neck was injected with 0.2 ml (200 μg of apitoxin) of bee-venom diluted with 1 ml of 2% lidocaine from session 1 to session 3.
Afficher plus [+] Moins [-]Monitoring of Anesthetic Depth with q-EEG (Quantitative EEG) in TIVA (Total Intravenous Anesthesia) and VIMA (Volatile Induction/Maintenance Anesthesia)
2006
Lee, S.H. (Konkuk University, Seoul, Republic of Korea) | Noh, G.J. (Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea) | Chung, B.H. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
To evaluate method for monitoring anesthetic both with quantitative electroencephalography (q-EEG), we recorded processed EEG (raw EEG) and pain till 100 minutes in beagle dogs anesthetized for 60 minutes with propofol (n=5, PRO group), isoflurane (n=5, ISO group) and propofol-ketamine-fentanyl (n=5, PEK group). Raw EEG was converted into 95% spectral edge frequency (SEF) by fast Fourier transformation (FFT) method. We investigated anesthetic depth by comparing relationship (Pearson's correlation) between q-EEG (95% SEF) and pain score.
Afficher plus [+] Moins [-]A Case of Protein Losing Nephropathy in a Dog Infected with Canine Babesia Gibsoni
2006
Kim, H.J. (Konkuk University, Seoul, Republic of Korea) | Park, C. (Konkuk University, Seoul, Republic of Korea) | Jung, D.I. (Konkuk University, Seoul, Republic of Korea) | Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Kim, J.W. (Konkuk University, Seoul, Republic of Korea) | Lim, C.Y. (Konkuk University, Seoul, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
A 1-year and 6-month-old, intact male, Shit-tzu dog was referred to the Konkuk University Veterinary Teaching Hospital, due to ascite, melena, severe anemia, and polyuria/polydipsia. Complete blood count showed moderate leukocytosis, lymphocytosis, monocytosis, and microcytic hypochromic nonregenerative anemia. On serum biochemistry profiles, hypoalbuminema and hypoproteinemia were observed, and proteinuria was detected on urinalysis.
Afficher plus [+] Moins [-]The Roles of PKC-δ on the Regulation of Insulin-Like Growth Factor (IGF)-I and Insulin-Like Growth Factor Binding Protein-3 Secretion by All-Trans Retinoic Acid in MCF-7 Cell
2006
Lee, S.M. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, S.H. (Chonbuk National University, Jeonju, Republic of Korea) | Choi, K.S. (Woosuk University, Wanju, Republic of Korea) | Kang, C.W. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
All-trans retinoic acid (AtRA) induces growth inhibition and apoptosis in a variety of tumer cells, including MCF-7 cells. Insulin-like growth factors (IGFs) system has been reported to be associated with the development of cancer. Although MCF-7 cell with AtRA is to be the major stimulus for the cell growth and apoptosis, the mechanism of insulin-like growth factor-I (IGF-I)/insulin-like growth factor binding protein-3 (IGFBP-3) system remains to be elucidated. Thus, this study was conducted to the effect of AtRA on the gene expression and level of IGF-I and IGFBP-3.
Afficher plus [+] Moins [-]Epidemiological Characteristics of 2002 Outbreak of Classical Swine Fever in Korea
2006
Park, C.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Wee, S.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, E.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, H.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Moon, O.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Choi, E.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Nam, H.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
This paper described the epidemiological characteristics of 2002 outbreak of classical swine fever (CSF) in Korea. A total of thirteen CSF-infected farms could be classified into two clusters according to the location and time of outbreak. Two farms located in the same county of Gangwon province and 11 farms located in several different districts of Incheon metropolitan/Gyeonggi province were identified as CSF-infected from April 16 to 30 and from October 7 to December 21 in December 21 in 2002, respectively.
Afficher plus [+] Moins [-]Protective Immunity Induced by Recombinant Outer Membrane Protein H of Pasteurella Multocida (A:3) of fowl Cholera in Mice
2006
Kim, Y.W. (Sungkyunkwan University, Suwon, Republic of Korea) | Yang, J.S. (Sungkyunkwan University, Suwon, Republic of Korea) | Kwon, M.S. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: [email protected]
Pasteurella multocida is a terrible veterinary pathogen that causes widespread infections in husbandry. To induce homologous and/or heterologous immunity against the infections, outer membrane protein Hs (OmpH) in the envelope of different strains of P. multocida are thought to be attractive vaccine candidates. Previously we cloned and characterized a gene for OmpH from pathogenic P. multocida (A:3) (In Press, Korean J. Microbiol. Biotechnol. 2005, 33, December).
Afficher plus [+] Moins [-]Encephalitic Listeriosis in a Korean Native Goat (Capra Hircus)
2006
Kim, H.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Jung, B.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Byun, J.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, O.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
This paper describes a naturally occurring case of meningoencephalitis associated with Listeria monocytogenes in a 4 month-old Korean native goat. The goat was raised in a herd consisting of seventy goats, and fed on straw and leftover vegetables including onions, cauliflower, and bean sprouts. The clinical symptoms of the goat were depression, inappetence, anorexia, circling, and recumbency before death. No conspicuous gross lesions were found except consolidation of the lung.
Afficher plus [+] Moins [-]Simulation Model-Based Evaluation of a Survey Program with Reference to Risk Analysis
2006
Chang, K.Y. (Animal Health Division, Livestock Bureau, Ministry of Agriculture and Forestry, Gwacheon, Republic of Korea) | Park, S.I. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
A stochastic simulation model incorporated with Reed-Frost approach was derived for evaluating diagnostic performance of a test used for a screening program of an infectious disease. The Reed-Frost model was used to characterize the within-herd spread of the disease using a hypothetical example. Specifically, simulation model was aimed to estimate the number infected animals in an infected herd, in which imperfect serologic tests are performed on samples taken from herds and to illustrate better interpreting survey results at herd-level when uncertainty inevitably exists.
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