Covalent coupling of proteins to different strains of bacteria modified via surface oxidation

Nedic, O.; Lalic, R.; Lako, B.; Balaz, J.

Covalent coupling of proteins to different strains of bacteria modified via surface oxidation

1995

Nedic, O. (Institute for the Application of Nuclear Energy, Belgrade - Zemun (Yugoslavia)) | Lalic, R. | Lako, B. | Balaz, J.

Six different bacteria were investigated as protein carriers. Carbohydrate structures of the cell wall were oxidized and human IgG (immunoglobulin G), as a model protein, was bound covalently through Schiff base linkage. The resulting bacteria - protein conjugates were characterized in terms of the amount of hIgG (human immunoglobulin G) coupled per mass unit of bacteria. Agglutination reactions with sheep anti - hIgG (human immunoglobulin G) antiserum illustrated the potential application of this protein immobilized on bacteria as a solid support.

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