Shoot bud formation and plantlet regeneration from the basal tissues of mulberry [Morus alba] leaves
1998
Sugimura, Y. (Kyoto Inst. of Technology (Japan)) | Adachi, T. | Kotani, E. | Furusawa, T.
Shoot bud formation was induced in the basal tissues of mulberry leaves using a two-step culture system: For achieving a high frequency of multiple shoot bud formation, it was essential that seedlings were cultured on a medium supplemented with a combination of 6-benzylaminopurine (BA) and 2, 3, 5-trilodobenzoic acid (TIBA), where after the basal leaf tissues were excised from the seedlings and cultured on a medium containing both thidiazuron (TDZ) and TIBA. It was clearly demonstrated that TIBA exerts a promotive effect on the induction of shoot buds when applied together with cytokinin. The shoot buds were formed as a mass on the entire surface of the midrib under the above mentioned conditions. From the shoot buds thus obtained, complete plantlets with profuse adventitious roots were produced in vitro
Показать больше [+] Меньше [-]