Evaluation of in vitro antioxidant and antidiabetic activities from Amomum nilgiricum leaf extract

Srinivas Chowdappa; Soumya Krishnamurthy; Kusuma Chathrapalya Gangadharaiah; Udayashankar C Arakere; Narasimhamurthy Konappa; Vinod Gubbiveeranna; Nagaraju Shiviaha; Niranjana Siddapura

Evaluation of in vitro antioxidant and antidiabetic activities from Amomum nilgiricum leaf extract

2021

Srinivas Chowdappa (Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India) | Soumya Krishnamurthy (Field Marshal K.M. Cariappa College, A Constituent College of Mangalore University, Madikeri 571 201, Karnataka, India) | Kusuma Chathrapalya Gangadharaiah (Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India) | Udayashankar C Arakere (Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India) | Narasimhamurthy Konappa (Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India) | Vinod Gubbiveeranna (Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India) | Nagaraju Shiviaha (Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India) | Niranjana Siddapura (Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India)

In the present study, hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic efficacy, scavenging activities, followed by estimation of total phenol, total flavonoids. In the present study, a significant amounts of total phenolics (79.92±1.58 mg/g) and flavonoids (21.74± 0.89 mg/g) were showed from Ethyl acetae fraction. H2O2 scavenging activity (97.62±2.89%) with IC50 value of 78.57 µg/ml concentrations. Ethyl acetate fraction exhibited maximum inhibitory activity of glucose movement into outer solution across dialysis membrane at 250 µg/ml as compared to the control. The ethyl acetate fraction revealed maximum insulin secretory activity (130.5±3.66%) in RIN-m5F cells. Methanol fraction recorded maximum glucose uptake percent in yeast cells (67.08±1.68%) when compared to standard metronidazole (68.06±0.73%). This study scientifically validates the antidiabetic activity of A. nilgiricum. Hence, in view of its comparative hypoglycemic strength, it can work as a valuable healing agent in treating diabetes.

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