MYC Induces a Hybrid Energetics Program Early in Cell Reprogramming

Prieto Martínez, Javier; Seo, Arnold Y.; León Rodríguez, Marian; Santacatterina, Fulvio; Torresano, Laura; Palomino-Schätzlein, Martina; Giménez, Karen; Vallet-Sánchez, Azahara; Ponsoda i Martí, Xavier; Pineda Lucena, Antonio; Cuezva, José M.; Lippincott-Schwartz, Jennifer; Torres Ibáñez, José Manuel

MYC Induces a Hybrid Energetics Program Early in Cell Reprogramming

2018

Cell reprogramming is thought to be associated with a full metabolic switch from an oxidative- to a glycolytic-based metabolism. However, neither the dynamics nor the factors controlling this metabolic switch are fully understood. By using cellular, biochemical, protein array, metabolomic, and respirometry analyses, we found that c-MYC establishes a robust bivalent energetics program early in cell reprogramming. Cells prone to undergo reprogramming exhibit high mitochondrial membrane potential and display a hybrid metabolism. We conclude that MYC proteins orchestrate a rewiring of somatic cell metabolism early in cell reprogramming, whereby somatic cells acquire the phenotypic plasticity necessary for their transition to pluripotency in response to either intrinsic or external cues.

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