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OPTIMUM CROPPING STRUCTURE OF DAKAHLIA GOVERNORATE
2019
T. Al-Zahaf | M. Rajab
It is the correct cropping structure that achieves the compatibility between more than one goal to reach the most efficient cropping structure in achieving and rationalizing water consumption and low use in the context of a set of constraints and determinants of productivity. In order to increase farm income and provide a large amount of irrigation water to benefit from the surplus In horizontal expansion. The model of the analysis of the agricultural structure in Dakahlia governorate included 18 crops through five different models. The first model (alternative) achieved this total yield of 6.780 billion pounds, more than the equivalent of 116 million pounds. Of the proposed model is less than its equivalent in the prevailing crop structure of 81 million m3 as a result of the increase in the area of some of the crops grown from their counterparts in the dominant structure. In accordance with Ministerial Decision No. 28 of 2018 issued by the Minister of Irrigation published in Al-Ahram newspaper on 8/3/2018, the year 142 - No. 47939, reducing the area of rice in Dakahlia governorate by 45%, four other models were reached.
Показать больше [+] Меньше [-]DEVELOPMENT OF IN VITRO SOMACLONAL VARIATION FOR PELARGONIUM GRAVEOLENS L’HERIT, AIT
2019
Rasha Hariedy | M. Rashed | Mahassen Sidky
In vitro somaclonal variation via callus is considered as one of the most effective tool for improvement of vegetative propagated crops. Different combinations of cytokinine (BA) and auxine (NAA) were used to induce somaclonal variation from leaf explants of the Egyptian geranium cultivar, Pelargonium graveolens L’Herit, Ait. El Qanater El khayria cv.8. Callus induction and regenerated shoots from callus were evaluated; 2.5 mg/L BA and 0.5 mg/L NAA was the optimal for callus induction and number of regenerated shoots. The obtained plantlets were acclimatized under glasshouse conditions with 80% to 90% survival and were transferred to the open field. The evaluation of growth traits and oil analysis were done for the new 13 clones compared with the mother plant in randomized block design for two successive seasons. Characterization of these new clones for morphological traits and essential oil yield and quality demonstrated the induction of variability in negative and positive directions in some characteristics in comparison with the mother plant. Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant and other new clones with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality. The genetic variation of the new geranium clones were tested using SRAP technique based on PCR, the polymorphism was detected using 16 pairs of SRAP primers in combinations as forward and reverse. The genetic differences between new clones and the mother plant were ascertained with SRAP primer pair (me2 em4); a unique profile for clone no.7 was detected in SRAP analysis. The similarity percentage varied between 66.7% to 100% homologies among the new clones and their mother plant. The presence of genetic variation between new clones and the mother plant proved that the calliclones were somaclonal mutants, and the geranium cultivar, El Qanater El khayria cv.8, was improved for high yield in herb and essential oil.
Показать больше [+] Меньше [-]COMPARATIVE STUDIES ON SOME PRODUCTIVE TRAITS AND GENETIC DIVERSITY OF TWO LOCAL STRAINS OF DUCKS: SUDANI AND DOMYATI
2019
Lamiaa Radwan | H. Madian | M. Mahrous | Y. Badaw | A. Zein El-Dein
The present study aimed to investigate the Genetic Diversity and productive Performance for Some Duck Local strains (sudani and domiaty) using Microsatellite genotyping analysis. From the hatched duck chicks, fifty day old ones were taken at random from each strain and reared under the same environmental, managerial and hygienic conditions from one day old until they aged 12 weeks (marketing age). The comparison was held between the two local duck strains for the following traits: Body weight and body measurements measured biweekly from day old until 12 weeks (pooled sex). Body measurements (shank length, keel length and body circumference) measured at 4,8,12 weeks of age for each strain. All chicks provided reared on floor pens, feed and water were provided ad-libitum all ducks chicks fed on two types of rations: A starter ration (from 0-4wks) and A finisher one (from 5-12 wks). The results indicated that There were significant differences in Body weight at all studied ages between Sudani and Domiaty strains (except at 2, 6 wks.) in hatching the average body weight of the Sudanese strain was 40.80 g however it was 34.12 g for the domyati strain, At 4 weeks age, the average body weight of Sudanese strain was 617.21g while it was 446.38g for the domyati strain, At 8 weeks age, the average body weight of Sudanese strain was1490.90g since it was 1235.98g for the domyati strain and At 12 weeks age, the average body weight of Sudanese strain was 2211.0g while was 1691.88g for the domyati strain. Also Body measurements of duck during early periods of growth (aged 4, 8 and 12 weeks) was At 4 wks age there were no significant differences between the two strains, At the age of 8 wks, the same trend was observed concerning the absence of the significant differences between strains and At the age of 12 wks, there were significant differences between the two strains of the breast circumference only where the superiority was recorded for the domyati strain, This may be reflected the genetic differences between the two strains. The results appeared that the PIC (polymorphic information content) of most microsatellite sites was lower than 0.5. This means that the selected microsatellite loci had a1ow diversity and can reflect the genetic relationship among Sudani and Domiaty populations. This assured that both sudani and domyati populations could be considered as a near populations on a molecular level.
Показать больше [+] Меньше [-]DEVELOPMENT OF SOME TOLERANT YEAST (SACCHAROMYCES CEREVISIAE) STRAINS TO HEAT AND SALT STRESSES
2019
Sara Abd El-Maksoud | M. Sayed | Fatma Badawy | Eman Fahmy
All living organisms are subjected to changing in conservational conditions, to which they must adapt to. Stress is defined as a threat refers to the physiological balance of systems critical to survival organisms. Five yeast strains (Saccharomyces cerevisiae) were subjected to different adverse environmental situations, such as thermal, osmotic and oxidative (salt) stresses. The objective of this work was to detect the most tolerant yeast strains under salt or heat stresses. Five yeast strains were exposed in a first experiment to heat stress at 20°C or 40°C beside to the control at 30°C to detect the more tolerant strain. The same yeast strains were subjected in a second experiment to two different concentrations of salt stress (NaCl); 0.5 or 1.0 M, separately, for two days (at 30°C as normal temperature for growth), other strains were exposed to 0.5 M concentration of NaCl for 24 hours, then 1 M for another 24 hours. For the heat stress results, strain S4 was more tolerant at 40oCwith insignificant difference compared to the control (30oC), while it showed significant difference at 20°C. Strain S5 also was more tolerant at 20oC with insignificant difference compared to the control. For salt treatment, the only insignificant value was for strain S3at 0.5M NaCl compared with the control.
Показать больше [+] Меньше [-]ROLE OF POLLINATORS ON EGYPTIAN CLOVER POLLINATION WITH SPECIAL REFERENCE TO HONEYBEE AT SOHAG GOVERNORATE, EGYPT
2019
A. Mazeed | E. Zidan | A. Abd El-latif
Data revealed that Egyptian clover were visited by 22 genus of arthropods belong to 16 families and 7 orders. The identified species were classified to phytophagous, Nectarivore, predatory insects. During both seasons Apis mellifera L. and Megachile sp. were the most abundant pollinators detected at the first week of May till the end of flowering season in mid-June. The highest activity period for A. mellifera was detected at 11:00 am. However, the lowest activity was found at 3:00 pm. and 5:00 pm. in both seasons, respectively, with insignificant difference between them in the first season. Whereas the highest activity period for Megachile sp. was detected at 1:00 pm. and the lowest activity was found at 7:00 am. During both seasons. The free pollination treatment produced the highest mean number of seeds per head followed by the caged with honeybees followed by the caged without honeybees treatment with average (48.15, 43.85 and 13.20 seeds /head) and (51.10, 46.10 and 10.85 seeds /head) during 2017 and 2018 seasons, respectively. The caged plants without honeybees produced the low weight of 1000 seed. Whereas the non-caged treatment produced the highest weights with an average (2.75 and 2.55 g) and (3.50 and 3.90 g) during both seasons, respectively.
Показать больше [+] Меньше [-]ASSESSMENT OF CYTOTOXIC AND ANTICANCER ACTIVITY OF Zygophyllum album AND Suaeda palastina EXTRACTS ON HUMAN LIVER CANCER CELL LINES
2019
Marwa El-Attar | A. Awad | F. Abdel-Tawab | H. Kamel | S. Ahmad | Amal Hassan
In this study, anticancer activity of Zygophyllum album and Suaeda palastina extracts was evaluated. Dichloromethane, methanol and hot water were used as solvents for extraction. Results indicated that the highest half-maximal inhibitory concentration (IC50) on human lung carcinoma (A549) cell lines was achieved by dichloromethane extracts of Z. album and S. palastina (70.48 μg/ml and 34.82 μg/ml respectively) compared to methanolic and hot water extracts. Furthermore, dichloromethane extracts of both plants had antiproliferative effect and highly cytotoxicity on human cancer cells. IC50 of Z. album was 27.74 μg/ml in the human hepatocellular carcinoma (HepG2), while IC50 of S. palaestina was 30.76 μg/ml with no cytotoxic activity on normal cell lines. In conclusion, these results suggest that Z. album and S. palaestina could be a good candidate species as a natural source of anticancer agents.
Показать больше [+] Меньше [-]ASSESSMENT OF GENOTОXIC EFFECTS OF SOME FOOD ADDITIVES ON SOME HUMAN CANCER CELLS
2019
Shimaa Rashad | F. Abdel-Tawab | Eman Fahmy | A. Attallah | Ekram Ahmed | A. Haggran
Some food additives commonly used by humans have been recently proved to be mutagenic. It is of significant importance to evaluate their genotoxic effects, since they are frequently consumed by humans in their daily meals. In this study, we investigated the effects of monosodium glutamate (MSG), sodium benzoate (SB) and saffron on human cell lines; lung cancer (A549), breast cancer (MCF7), colon cancer (Caco-3) and normal lung (Wi38) cell line as control. Cytotoxicity of food additives was screened on multiple cell lines and examined by NR assay (Neutral Red assay). The present study focused on the cytotoxic activity of the food additives and its possible underlying mechanisms. The results showed that food additives; MSG, SB and saffron induced profound cytotoxicity in cancer cells of human colon cancer (Caco-3) (IC50 = 33.92, 15.01, 3.98 μg/ml), human breast cancer (MCF7) (IC50 = 12.79, 0.378, 10.73 μg/ml), human lung cancer cell line (A549) (IC50 = 27.37, 0.45, 2.46 μg/ml), respectively. Moreover, food additives exhibited cytotoxic activity on normal lung cell lines (Wi38) (IC50 = 4.25, 0.733, 18.14 μg/ml). These data indicated that food additives decreased cell viability in malignant and nonmalignant cells as well as confirmed the occurrence of their cytotoxic effects.
Показать больше [+] Меньше [-]EFFECT OF FOLIAR APPLICATION OF POTASSIUM SILICATE AND SOME POSTHARVEST TREATMENTS ON GROWTH, PRODUCTIVITY AND STORABILITY OF GARLIC
2019
Amany Abdel-Latif | Nadia Ibrahim | Shadia Ismail
Two experiments were carried out in the experimental farm of Al-Kanater Research Station at ElQalyubia Governorate, during the two consecutive years of 2016/2017 and 2017/2018 to investigate the effect of potassium silicate foliar application on growth, productivity and some postharvest treatments on storability of garlic ((Allium sativum L.)" clone sids 40". In the field experiment, the treatments comprised of control(water spray),foliar application of potassium silicate at 4000, 6000 and 8000ppm and plants were sprayed at 30, 60 and 90 days after planting. In the storage experiment, cured bulbs were stored at room temperature conditions and subjected to sixteen postharvest treatments included the previous four treatments (the three potassium silicate levels and control). In addition, three spraying treatments of sodium silicate at 6000 ppm, chitosan at 200 ppm and calcium chloride at 3000 ppm both separately and their combinations with potassium silicate (three treatments in field) were done. The results revealed that, the highest plant length, number of leaves, fresh, dry weight\plant, neck diameter, bulb diameter and chlorophyll content of garlic were achieved from foliar application of potassium silicate at the 4000ppm after 120 days from planting compared to the control. Foliar spray of potassium silicate at 4000 ppm recorded the highest total yield and bulb quality such as bulb diameter, bulb weight/plant, number of cloves, cloves weight, total soluble solids and dry matter of bulbs. Leaf K percentage significantly increased by spraying of potassium silicate at 6000ppm.Further, all potassium silicate treatments recorded higher silicon percentage over the control. Postharvest results showed that weight loss and shrinking percentages of the bulbs after 7 months of the storage were the least with the foliar application of potassium silicate at 8000ppm plus chitosan at 200ppm in storage followed by chitosan individually, potassium silicate at 8000 ppm and potassium silicate at 6000 ppm combined with calcium chloride as a postharvest treatment. However, the highest total soluble solids after 7 months of the storage was obtained from foliar spraying of potassium silicate at 4000 ppm combined with either sodium silicate or chitosan. Also, bulbs treated with foliar application of potassium silicate at 6000 or 8000 ppm combined with chitosan at 200 ppm, had the highest values of dry matter after 7 months from storage. In general, foliar application of potassium silicate at 4000ppm was the superior treatment for enhancing plant growth, yield and quality. Also, foliar application of potassium silicate at 8000ppm and sprayed with chitosan at 200 ppm was the most effective for garlic storability seven months at the room temperature conditions.
Показать больше [+] Меньше [-]EVALUATE THE EFFECT OF SOME FACTORS AFFECTING SOLUBILIZATION OF PHOSPHORUS IN RHIZOSPHERE
2019
rookaya el-hag | A. Elgala | M. Elsharawy | M. Eid
The aim of this work was to conduct pot experiment to study the ability of plant roots on solubilizing various sources of phosphorus and factors that may facilitate or inhibit their activity. A split medium – split root technique experiment was conducted to study the effect of CaCO3, pH, organic matter (humic acid) and bentonite on the pH, P solubility in the medium and P concentration in bean plants (Vicia faba var. balady). The changes in pH of the lower solution was recorded, also the root exudate was collected in 500 ml of CaCl2 solution 0.5 × 10-4 M and pH 6.85. Results showed that the highest recorded total dry weight was found when both N forms were applied in the ratio of 1:4 NO-3: NH+4 or NO-3 alone. The mechanism of solubilizing rock phosphate by exudating protons or organic, amino and other organic compounds is possible at the root surface even in alkaline soil as long as the rock phosphate material was added near the root and organic matter was added to limit the fixing power of the inorganic components as CaCO3 and excess soluble Ca. Also, the availability of P from rock phosphate sources depends on its reactivity value.
Показать больше [+] Меньше [-]EFFECT OF IRRIGATION SYSTEMS IMPROVEMENT ON WATER UNIT PRODUCTIVITY UNDER NORTH DELTA REGION CONDITIONS OF EGYPT
2019
Khaled Shabaan | M. Galal | T. Elgamal
Several field trials and laboratory studies were conducted to evaluate the impact of the implementation of the activities and processes of the development of the field irrigation systems at ElMahmoudia area, El-Beheira Governorate, Egypt, during the successful growing seasons winter 2016/17 and summer 2017 to investigate the effect of irrigation systems improvement projects on water productivity. The measurements were conducted in a tertiary canal (Mesqa) at the head of Nekla canal (Arafa Mousa Mesqa). Water consumption values for different crops were calcaulated, and water application was calculated through calibrating the capacities of the pumps and recording the operation hours. The most important results were as follows: Applied irrigation water decreased after irrigation improvement. Applied irrigation water for wheat, rice, maize, sweet potatoes and watermelon before irrigation improvement were higher than the corresponding values after irrigation improvement by 9.0%, 15%, 11%, 15% and 10% respectively. The productivity of wheat, rice, maize, sweet potatoes and watermelon increased after irrigation improvement by 7.0%, 4.0%, 3.0%, 8.0% and 9.0% respectively. Water productivity increased after irrigation improvement, and the increase ratios for wheat, rice, maize, sweet potatoes were 14.0%, 16.0%, 13.0%, 20.0%, and 18.0% respectively.
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