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Fluorophotometric evaluation of experimental blood-aqueous barrier disruption in dogs
1991
Ward, D.A. | Ferguson, D.C. | Kaswan, R.L. | Green, K. | Bellhorn, R.W.
A protocol for performing slit-lamp fluorophotometry of the anterior chamber in dogs was established. The technique was then used to develop a model of blood-aqueous barrier disruption that can be used for comparative testing of ophthalmic anti-inflammatory drugs. It was determined that barrier disruption induced by a slow, controlled paracentesis of a small volume of aqueous humor may provide the most reliable model for drug testing. Additionally, fluorophotometry proved to be a sensitive and accurate means of detecting breakdown of the blood-aqueous barrier.
Показать больше [+] Меньше [-]Enumeration of T and B lymphocytes in bovine leukemia virus-infected cattle, using monoclonal antibodies
1988
Williams, D.L. | Amborski, G.F. | Davis, W.C.
Monoclonal antibodies and microfluorimetry were used to determine the absolute number of B and T lymphocytes in the blood of bovine leukemia virus (BLV)-infected cows. The blood lymphocyte populations from BLV-infected cows were significantly higher than those from BLV-negative cows. The increase in the lymphocyte population in 3 BLV-infected nonlymphocytotic cows was attributed to a significant increase in the number of T lymphocytes; in 3 BLV-infected persistently lymphocytotic cows, the increase was attributed to a significant increase in the number of B and T lymphocytes. One persistently lymphocytotic cow had a high lymphocyte count, and lymphocytes from this cow contained cells that appeared to stain with markers specific for bovine B and T lymphocytes. We concluded that infection of cattle with the B-cell lymphotropic retrovirus, BLV, not only affected B cells, but also T cells.
Показать больше [+] Меньше [-]Spectrofluometry of sulfonamides using fluorescamine
1990
Lim, J.Y. | Han, S.N. | Lee, M.H. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Park, J.M. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
