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Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Полный текст
2018
Czyżewska-Dors Ewelina | Kwit Krzysztof | Stasiak Ewelina | Rachubik Jarosław | Śliżewska Katarzyna | Pomorska-Mól Małgorzata
Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Полный текст
2018
Czyżewska-Dors Ewelina | Kwit Krzysztof | Stasiak Ewelina | Rachubik Jarosław | Śliżewska Katarzyna | Pomorska-Mól Małgorzata
The aim of the study was to determine the effects of supplementation of sows’ and growing pigs’ diets with three newly developed synbiotic and two extant commercial probiotic products on selected immune parameters under field conditions.
Показать больше [+] Меньше [-]Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Полный текст
2018
Czyżewska-Dors, Ewelina | Kwit, Krzysztof | Stasiak, Ewelina | Rachubik, Jarosław | Śliżewska, Katarzyna | Pomorska-Mól, Małgorzata
The aim of the study was to determine the effects of supplementation of sows’ and growing pigs’ diets with three newly developed synbiotic and two extant commercial probiotic products on selected immune parameters under field conditions. The study was performed on 30 sows and 48 piglets of the Danbred breed. Immune parameters such as concentration and proportion of white blood cells and their subpopulations, immunoglobulins amount in serum, and serum concentration of cytokines and acute phase proteins were recorded with the use of a haematology analyser and ELISA kits. No significant differences between treatment groups and controls were found with regard to the immune parameters evaluated except for serum immunoglobulin concentration, which was significantly increased by synbiotic products B and C and probiotic product D. The results of the study indicate that the synbiotic products B and C and probiotic product D are worthy of further investigation as promising candidates to improve the immune status of healthy sows and their offspring.
Показать больше [+] Меньше [-]Evaluation of intestinal damage biomarkers in calves with atresia coli Полный текст
2018
Yildiz Ramazan | Ok Mahmut | Ider Merve | Aydogdu Ugur | Naseri Amir | Parlak Kurtulus | Gulersoy Erdem
Evaluation of intestinal damage biomarkers in calves with atresia coli Полный текст
2018
Yildiz Ramazan | Ok Mahmut | Ider Merve | Aydogdu Ugur | Naseri Amir | Parlak Kurtulus | Gulersoy Erdem
Intestinal obstruction such as atresia coli causes pathophysiological changes in gastrointestinal tissue due to the rise of intra-abdominal pressure. The aim of this study is to determine the intestinal damage with intestinal biomarkers in calves with atresia coli.
Показать больше [+] Меньше [-]Evaluation of intestinal damage biomarkers in calves with atresia coli Полный текст
2018
Yıldız, Ramazan | Ok, Mahmut | Ider, Merve | Aydogdu, Ugur | Naseri, Amir | Parlak, Kurtulus | Gulersoy, Erdem
Intestinal obstruction such as atresia coli causes pathophysiological changes in gastrointestinal tissue due to the rise of intra-abdominal pressure. The aim of this study is to determine the intestinal damage with intestinal biomarkers in calves with atresia coli. The study was conducted on 40 Holstein calves diagnosed with atresia coli with mild to moderate abdominal distention and 10 healthy Holstein calves which served as the control. Blood samples were collected from all calves, and then serum concentrations of intestinal biomarkers were estimated, namely intestinal fatty acid binding protein (IFABP), liver fatty acid binding protein (LFABP), trefoil factor 3 (TFF3), and intestinal alkaline phosphatase (IAP), using commercially available specific bovine ELISA kits. An automatic blood gas analyser was employed for determining the lactate concentration. The concentrations of serum LFABP (P < 0.01), IFABP, TFF3, IAP, and blood lactate (P < 0.001) were significantly higher in calves with atresia coli than in healthy calves. The calves affected with atresia coli exhibited severe intestinal damage, and IFABP, LFABP, and TFF3 have significant diagnostic importance and play a useful role in determining the intestinal damage due to intestinal obstruction. High levels of IAP and lactate may serve as a signal for the development of intestinal injury.
Показать больше [+] Меньше [-]Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Полный текст
2018
Weiner Marcin | Żukiewicz-Sobczak Wioletta | Tokarska-Rodak Małgorzata | Plewik Dorota | Pańczuk Anna | Siłuch Marta | Zagórski Jerzy | Sobczak Paweł | Chmielewski Tomasz | Tylewska-Wierzbanowska Stanisława | Shkilna Mariia | Korda Mykhailo | Klisch Ivan | Andreychyn Mykhailo | Pavliuk Mariana
Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Полный текст
2018
Weiner Marcin | Żukiewicz-Sobczak Wioletta | Tokarska-Rodak Małgorzata | Plewik Dorota | Pańczuk Anna | Siłuch Marta | Zagórski Jerzy | Sobczak Paweł | Chmielewski Tomasz | Tylewska-Wierzbanowska Stanisława | Shkilna Mariia | Korda Mykhailo | Klisch Ivan | Andreychyn Mykhailo | Pavliuk Mariana
Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks.
Показать больше [+] Меньше [-]Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Полный текст
2018
Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks. A total of 136 Ixodes ricinus ticks, collected in the Ternopil (Ukraine) region, including 126 adults (70 females and 56 males), and 10 nymphs were examined. The identification of the species and their developmental form was based on morphological characteristics. PCR with B5S-Bor and 23S-Bor primers resulted in Borrelia burgdorferi sensu lato DNA amplification among six ticks (4.4%). The detailed analysis based on the DNA sequencing showed the presence of DNA of Borrelia afzelii in four samples; the remaining two represented Borrelia burgdorferi sensu lato complex, although their genospecies were not determined. The research confirmed the dominance of Borrelia afzelii genospecies in the ticks from Ukraine. It seems reasonable to undertake similar research in ticks from other regions of Ukraine. Knowledge in this field can be useful for public health and planning the prevention of tick-borne diseases.
Показать больше [+] Меньше [-]Fatty acid profile in fat obtained from edible part of land snails harvested in Poland Полный текст
2018
Szkucik Krzysztof | Ziomek Monika | Paszkiewicz Waldemar | Drozd Łukasz | Gondek Michał | Knysz Przemysław
Introduction: The objective was to determine the content of fatty acids in edible snail fat by snail species, collection site, and processing stage.
Показать больше [+] Меньше [-]Coronaviruses in avian species – review with focus on epidemiology and diagnosis in wild birds Полный текст
2018
Miłek Justyna | Blicharz-Domańska Katarzyna
Coronaviruses in avian species – review with focus on epidemiology and diagnosis in wild birds Полный текст
2018
Miłek Justyna | Blicharz-Domańska Katarzyna
Coronaviruses (CoVs) are a large group of enveloped viruses with a single-strand RNA genome, which continuously circulate in mammals and birds and pose a threat to livestock, companion animals, and humans. CoVs harboured by avian species are classified to the genera gamma- and deltacoronaviruses. Within the gamma-CoVs the main representative is avian coronavirus, a taxonomic name which includes the highly contagious infectious bronchitis viruses (IBVs) in chickens and similar viruses infecting other domestic birds such as turkeys, guinea fowls, or quails. Additionally, IBVs have been detected in healthy wild birds, demonstrating that they may act as the vector between domestic and free-living birds. Moreover, CoVs other than IBVs, are identified in wild birds, which suggests that wild birds play a key role in the epidemiology of other gammaCoVs and deltaCoVs. Development of molecular techniques has significantly improved knowledge of the prevalence of CoVs in avian species. The methods adopted in monitoring studies of CoVs in different avian species are mainly based on detection of conservative regions within the viral replicase, nucleocapsid genes, and 3’UTR or 5’UTR. The purpose of this review is to summarise recent discoveries in the areas of epidemiology and diagnosis of CoVs in avian species and to understand the role of wild birds in the virus distribution.
Показать больше [+] Меньше [-]Coronaviruses in avian species – review with focus on epidemiology and diagnosis in wild birds Полный текст
2018
Miłek, Justyna | Blicharz-Domańska, Katarzyna
Coronaviruses (CoVs) are a large group of enveloped viruses with a single-strand RNA genome, which continuously circulate in mammals and birds and pose a threat to livestock, companion animals, and humans. CoVs harboured by avian species are classified to the genera gamma- and deltacoronaviruses. Within the gamma-CoVs the main representative is avian coronavirus, a taxonomic name which includes the highly contagious infectious bronchitis viruses (IBVs) in chickens and similar viruses infecting other domestic birds such as turkeys, guinea fowls, or quails. Additionally, IBVs have been detected in healthy wild birds, demonstrating that they may act as the vector between domestic and free-living birds. Moreover, CoVs other than IBVs, are identified in wild birds, which suggests that wild birds play a key role in the epidemiology of other gammaCoVs and deltaCoVs. Development of molecular techniques has significantly improved knowledge of the prevalence of CoVs in avian species. The methods adopted in monitoring studies of CoVs in different avian species are mainly based on detection of conservative regions within the viral replicase, nucleocapsid genes, and 3’UTR or 5’UTR. The purpose of this review is to summarise recent discoveries in the areas of epidemiology and diagnosis of CoVs in avian species and to understand the role of wild birds in the virus distribution.
Показать больше [+] Меньше [-]Perikarditis Travmatikalı Sığırlarda Venöz Kan Gaz Ve Asid- Baz Analizlerin Önemi Полный текст
2018
YILDIZ, Ramazan
Yapılan çalışma Perikarditis Travmatika (PT) olan sığırların rutin klinik muayeneleri esnasında alınan venöz kan gazlarınınincelenmesi sonucu oluşturuldu. Bu çalışmanın amacı kan gazı analizinin Perikarditis Travmatikalı sığırlarda önemini ortayakoymaktır. Çalışmada Perikarditis Travmatika tanısı koyulan 22 adet 2-4 yaş aralığında olan sığır kullanıldı. Kan gaz analizleriiçin V. Jugularisten 2,5 ml kan alınarak 15 dk. içinde kan gazı cihazında analiz edildi. Yirmi sığırın kan pH'sı normal (7.35-7.50)sınırlarında iken, bir hayvanda hafif asidemi (7.20-7.35), 1 hayvanda ise metabolik alkaloz ( > 7.50) tespit edildi. Kan pCO2değeri bir hayvan ( > 50 mmHg) haricinde normal referans (30-40 mmHg) alarığında olduğu görüldü. Kan pO2 değeri 11 hayvanda< 30 mmHg seviyesininnin altında olduğu tespit edildi. Laktat seviyesi 8 hayvanda normal sınırlarda (< 2 mmol/L) iken,5 hayvanda klinik uyarı (2-4 mmol/L) seviyesinde, 9 hayvanda kritik seviyenin (>4 mmol/L) üzerinde olduğu görüldü. Potasyumseviyesinin 17 hayvanda normal sınırların (<3.8 mmol/L) altındaydı. HCO3 değeri 7 hayvanda normal (20-30 mEq/L)sınırların üzerinde, 2 hayvanın ise normal sınırların altında olduğu ve bu iki hayvanda hiperlaktatemi olduğu görüldü. Yükseklaktat laktat ile düşük SatO2 ve pO2 parametreleri PT'li sığırlarda doku hipoksisi şekillendiğini göstermektedir. Sonuç olarakkan gazı ölçümünün hastanın tedavi seçenekleri ve prognostik takip için kullanılmasının anlamlı olabileceği kanısına varıldı.
Показать больше [+] Меньше [-]Yüksek Düzeyde Konsantre Yemle Beslenen Kuzularda Yeme Maya (Saccharomyces cerevisiae) veya Malik Asit İlavesinin Performans Üzerine Etkisi Полный текст
2018
ERDOĞMUŞ SÜER, İnci Nathalie | KOCABAĞLI, Neşe
Bu çalışmada, yüksek düzeyde konsantre yemle beslenen Kıvırcık kuzularda yeme maya (Saccharomyces cerevisiae-Levabon Rumen E; Biomin,Avusturya) veya malik asit (Rumalato®; Norel & Nature S.A., Barselona, İspanya; disodyum malat ve kalsiyum malat içermektedir(0,16:0,84, w/w)) ilavesinin performans üzerine etkisinin araştırılması amaçlanmıştır. Araştırmada 3 aylık yaşta 45 baş Kıvırcık ırkı erkek kuzukullanılmıştır. Hayvanlar ağırlıkları göz önünde bulundurularak, gruplar arasında istatistiksel olarak bir fark olmayacak şekilde biri kontrol,diğer ikisi deneme olmak üzere 3 gruba ayrılmıştır. Kontrol grubunun konsantre yemlerine hiçbir katkı maddesi eklenmemiş, deneme gruplarındanbirine 1,5 g/kg KM düzeyinde canlı maya kültürü, diğerine ise 5,0 g/kg KM malik asit tuzu ilave edilmiştir. Hayvanlar 60 gün süreile besiye alınmıştır. Bu süre boyunca hayvanların sürekli olarak temiz içme suyuna erişimleri olmasına dikkat edilmiş ve ad libitum olarak(%80: %20) konsantre yem:kuru ot karması ile beslenmişlerdir. Besi süresince kuzuların canlı ağırlıkları denemenin 0, 15, 30, 45 ve 60. günlerindesabah aç karnına yapılan tartımlarla saptanmış, verilen yemler her gün, artan yemler ise haftalık olarak tartılmıştır. Deneme sonundaher gruptan 7 baş kuzu kesilerek sıcak ve soğuk karkas ağırlıkları ile karkas randımanları saptanmıştır. Yem tüketimi ve yemden yararlanmaoranları grup beslemesi yapıldığı için istatistiksel olarak değerlendirilmemiştir. Canlı ağırlık ve günlük canlı ağırlık artışlarında gruplar arasındaönemli bir fark gözlenmemiştir (p > 0,05). Sıcak ve soğuk karkas ağırlıkları ve karkas randımanları gruplar arasında istatiksel açıdan anlamlıfark göstermemiştir. Sonuç olarak, bu çalışmada kuzularda yeme maya veya malik asit ilavesi performans üzerine istatistiksel olarak önemlibir etki göstermemiştir.
Показать больше [+] Меньше [-]Kaynak Alabalığının Salvelinus fontinalis Semeninin Bakteriyel Florasının Belirlenmesi Полный текст
2018
Ertekin, Özlem | Kutluyer, Filiz | Kocabaş, Mehmet | Başçınar, Nadir
Experiments were designed to evaluate bacterial flora in semen culture of brook trout (Salvelinus fontinalis). Herein, semen samples werecollected from fish and standard microbiology techniques were processed for bacterial flora. The samples were serially diluted in physiologicalsaline (NaCl: 0.85%). Aliquots of 0.1 ml of each dilution were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose BengalAgar (RBC) (Yeast-Mold Count), VRBD (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count).Micrococcus/Staphylococcus group bacteria (11.11%) were counted from brook trout (S. fontinalis) semen. Total bacteria count varied between103-105 cfu ml-1 in semen.
Показать больше [+] Меньше [-]Comparative Toxicity of Paraquat and 2, 4-Dichlorophenoxy Acetic Acid in Adult Artemia franciscana Полный текст
2018
Rahnama, Roya | Tulaby Dezfuly, Zahra | Alishahi, Mojtaba
Herbicides are being used widely in agriculture and aquaculture for controlling noxious weeds. Paraquat and 2, 4-Dichlorophenoxyacetic acid(2,4-D) have been the most widely used herbicide during the past three decades. Toxicological properties of Paraquat are attributed to its abilityto produce reactive oxygen species such as superoxide anion that may directly or indirectly cause cell death. 2,4-Dichlorophenoxyacetic acid(2,4-D) is a broad-leaf, systemic, phenoxy herbicide used as the active ingredient in several commercially available aquatic herbicide products.Bioassay technique has been the cornerstone of programs on environmental health and chemical safety. The application of environmentaltoxicology studies on non-mammalian vertebrates is rapidly expanding. So the present study investigated the acute toxicity of Paraquat and2,4- Dichlorophenoxy acetic acid (2,4-D) as aquatic ecosystems pollutants on Artemia franciscana. Artemia is one of the most suitable testorganisms available for ecotoxicity testing and research and most commonly used live food in aquaculture. Acute toxicity (48 h LC50) of twoherbicides (Paraquat, 2, 4-dichlorophenoxy acetic acid) was determined. A. franciscana exposed to Serial concentrations of both mentionedherbicides. Mortalities at 12, 24, 36 and 48 hours after exposure were recorded and LC50 were calculated using Probit software. The resultsobtained indicate that the acute toxicity of these herbicides is significantly different in adult A. franciscana. The lethal concentration of Paraquatand 2,4-D were calculated 2.701, 14.475 mg/L in A. fransiscana respectively. So The LC50 of two examined herbicides was significantly differentand the mortality rate was increased by increasing exposure time. Finally, these data support the hypothesis the possible risks associatedwith the presence of herbicides particularly Paraquat residues in the aquatic animals and their environment.
Показать больше [+] Меньше [-]In-Situ Transitional Cell Carcinoma of Urinary Bladder in a Cat Полный текст
2018
ÖZTÜRK, Hazal | Egeden, Evrim | Egeden, Özlem | Gürel, Aydın
Urinary bladder tumors in cats, unlike in dogs, usually appear outside of the trigonal region as localization. These tumors are confused withurinary tract infections associated with hematuria in a clinical sense. Cytological examination and ultrasound (USG) imaging techniques arevery valuable, but histopathological approach is the golden key. In this case neutered male, mixed, 17-year-old cat was brought to the clinicwith non-obstructive hematuria attacks. A superficial hypoechoic mass located at the apex of the urinary bladder was detected in the USG imagingtechnique. No discernible findings were found by cytological examination. After removal of the suspected area, the biopsy specimen waspresented to the pathology department for histopathological examination. Histopathological examination revealed transitional cells coveringentire surface of the mucosal epithelium and showing growth into the lumen, and they were characterized by mild anisocytosis and anisokaryosis.The patient was diagnosed as in-situ non-papillary, non-infiltrating type of transitional cell carcinoma (in-situ carcioma). These tumorsare quite rare and have better prognosis. The condition of the patient was completely resolved without medical treatment in the postoperativeperiod. When this case report was prepared, 6 months after the operation, there was no recurrence in the patient. Contrary to infiltrative urinarybladder tumors, in-situ carcinoma could be completely cured by surgically, therefore the case was found worthy to be presented.
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