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A Current Approach in Animal Breeding: CRISPR/Cas9 Genome Modification System Полный текст
2016
Fatih Bilgi | Zeynep Demirtaş | Levent Mercan
Genome modifications include potential about providing significant advantages on increasing yield performance and developing resistance to diseases. Gene editing methods that provides silencing or expressing of a gene which is an individual already has, have important potential for improving genetic structure without environmental effects. In recent times, new gene editing systems were developed. These are ZFNs (Zinc Finger Nucleases), TALENs (Transcription Activator-like Effector Nucleases) and CRISPR/Cas nuclease systems. CRISPR/Cas system is a microbial immune system that uses RNA guided nucleases for destroying genetic materials and its potential usage like a simple and efficient gene editing mechanism in animals is being evaluated recently. In this review, we summarized CRISPR/Cas9 system and its usability in animal breeding
Показать больше [+] Меньше [-]Antimicrobial Activities of Five Strains Of Lactococcus Isolated from Beef Against Indicator Organisms of Public Health Significance Полный текст
2016
Olusegun Ayodele Olaoye
Antimicrobial Activities of Five Strains Of Lactococcus Isolated from Beef Against Indicator Organisms of Public Health Significance Полный текст
2016
Olusegun Ayodele Olaoye
Five strains of Lactococcus, including L. garviae K2, L. piscium SU4, L. lactis subsp. cremoris E22, L. plantarum L7 and L. lactis subsp. hordinae E91 were screened for production of antimicrobial agents. The strains were also analysed for antimicrobial activities against spoilage and pathogenic organisms, including Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Yersinia enterocolitica, Pseudomonas aeruginosa, Klebsiella pneumoniae, Listeria monocytogenes, Bacillus cereus and Pseudomonas fluorescens. Result revealed that L. piscium SU4, L. plantarum L7 and L. lactis subsp. cremoris E22 had lactic acid production (g/107 colony forming units, CFU) of 7.23, 7.20 and 7.19. The value of 6.83 recorded as lactic acid produced by L. garviae K2 was significantly different from those obtained for others. The highest acetic acid production (3.55 g/107 CFU) was recorded for L. garviae K2 while L. piscium SU4 had the lowest (2.99 g/107 CFU). L. lactis subsp. hordinae E91 had diacetyl production of 71.99 (µg/107 CFU), which was higher than those recorded for other Lactococcus strains. Test for antimicrobial activities showed that Escherichia coli NCTC 86, Yersinia enterocolitica NCTC 10460, Pseudomonas aeruginosa NCIMB 10848, Bacillus cereus NCTC 21113 and Pseudomonas flourescens recorded higher susceptibilities to the antimicrobial action of the Lactococcus strains than others; zones of inhibition of 5 mm and above were recorded for the indicator organisms compared to lower values (
Показать больше [+] Меньше [-]Efecto de lixiviados del raquis de plátano sobre la actividad y biomasa microbiana en floración y cosecha del tomate Полный текст
2005
Muñoz V., Rosa Elvira | Madriñán Molina, R.
Se utilizaron cinco tratamientos: el testigo (T0) y cuatro concentraciones de lixiviados (T1= 100%, T2= 75%, T3= 50% y T4= 25%) aplicados 15, 30 y 60 días después del trasplante. La actividad microbiana se determinó con la metodología del CAB y la biomasa microbiana con el método de fumigación-extracción. En la actividad microbiana se encontraron diferencias significativas entre los tratamientos, presentando la aplicación del lixiviado a la menor concentración (25%) la mayor actividad (56.76 mgCO2g suelo-1). La actividad fue mayor en floración en todos los tratamientos. Para biomasa microbiana no se presentaron diferencias significativas entre los tratamientos ni entre épocas. ABSTRACT The effect of leaching from rachis on soil microbial biomass and activity during tomato flowering and harvesting period. Field trail comprised five treatments of different leaching concentrations (T0= test, T1= 100%, T2= 75%, T3=50%, and T4=25%) sprinkled on soil 15, 30 and 60 days after tomato transplanting. Microbial activity was measured with CAB method and microbial biomass was recorded with fumigation - extraction method. The average microbial activity with sprinking of 25% of leaching was higher (56.76 mgCO2g suelo-1). No significant difference in order to treatments and periods in microbial biomass were observed. These observations showed leaching sprinkling in low concentration influence en microbial activity for the conditions of this experiment. Key words: Soil biology, biomass, leaching, Lycopersicum sculentun.
Показать больше [+] Меньше [-]Physicochemical Analysis of Water Quality of Brook Kuruçay Полный текст
2016
Ekrem Mutlu | Arzu Aydın Uncumusaoğlu
In this study, through the analyses of water samples taken from 9 stations on the brook between July 2012 and June 2013, we aimed to determine the monthly and seasonal changes in water quality parameters of Brook Kuruçay, to determine the water quality properties, to reveal the pollution problems, to determine the suitability level in terms of aquatic life and to classify the quality of water in accordance with Surface Water Quality Management Regulation’s Inland Surface Water Classes criteria. The study area is located southeast of the Hafik District of Sivas city and the altitude is 2608 m. The water samples were collected from 9 stations established on the brook, and some physicochemical parameters and heavy metal concentrations were analyzed in water samples. The cleaning and maintenance of all of the equipment, land-type measurement tools, and glass sampling containers to be used in sampling were made 1 day before sampling. Sampling tubes were immersed into 15 cm below the water surface for taking water samples. Heavy metal concentrations were determined in the Sivas Provincial Control Laboratory in the same day with sampling (within 5 hours). The total alkalinity, total hardness, ammonium nitrogen, nitrite, nitrate, ammonium azote, phosphate, sulfite, sulfate, chloride, sodium, potassium, suspended solid matter (SSM), chemical oxygen demand (COD), biological oxygen demand (BOD), calcium, magnesium, ferrous, lead, copper, zinc, nickel, mercury and cadmium analyses of water samples were performed. As a result of the analyses, it was determined that, since Brook Kuruçay falls into the water resource class, which is the most sensitive to pollution, the water quality of the brook should be monitored regularly.
Показать больше [+] Меньше [-]Marketing Structure, Problems and Suggestions of Small Ruminant Production in Çamardı District of Niğde Province Полный текст
2016
Arzu Seçer | Mustafa Boğa
In Turkey, there are 31.1 million head sheep in 2014. Central Anatolia Region has 8.3% of this total number. Nigde is the most important sheep producer province in this region. In this study it is aimed to determine marketing structure of products obtained from sheep production activities, define problems of it and present solutions for these problems. The main material of the research was consisted of primary data obtained from 39 Akkaraman sheep producers in Çamardı district in Nigde. In the sheep farms, milk production yield was 1374.4 kg per year. As 73.5% of this yield was presented to market, rest of it was used for self-consumption. Milk was generally sold to local traders. In the operations, livestock also was sold. When 89.7% of livestock sold to kasaps 10.3% of it was sold to local traders. It can be said that producers who are the members of cooperatives were very low and 51.3% of the producers stated that cooperatives can be useful for the solutions of marketing problems of sheep products. Main expectations of producers from cooperatives were providing them market alternative, providing easiness for processing of products and leading to increasing numbers and kinds of buyers in the market.
Показать больше [+] Меньше [-]Usage of Edible Mushrooms in Various Food Products Полный текст
2016
Özge Süfer | Fuat Bozok | Hande Demir
Using of edible mushrooms which are generally consumed in houses in dried form is based on mainly instant soup and sauce formulations. Recently, the cultivations of Agaricus bisporus and Pleurotus ostreatus species have become widespread. Utilization of these cultivated mushrooms in recipes would bring added value to related food products. For this purpose, Agaricus bisporus and Pleurotus ostreatus species farmed in Osmaniye Korkut Ata University Mushroom House were dried and then pulverized. Firstly, a snack was prepared with Agaricus bisporus powder. Agaricus bisporus powder was substituted for wheat flour at the rates of 5 %, 10 %, 20 % and 30 % and thus the potential of food product which had relatively lower carbohydrate and fat level and higher fiber content was investigated. In the second part of the study, either 5 %, 10 % of Agaricus bisporus powder or 5 %, 10 % of Pleurotus ostreatus powder were added into traditional Turkish meatball (beef mince, salt) which was cooked in conventional oven, so meat flavor could be replaced by herbal flavor coming from mushroom. This property mat obey the purpose that, the created new product will be consumed fondly especially by children. Sensory and physical (colour and texture) analysis were performed in both snack and meatball samples and the results were evaluated statistically.
Показать больше [+] Меньше [-]Effect of Drying Methods and Pre Treatments on Nutritional Value and Sensory Quality of Fish (Oreochromis niloticus, Pylodictis olivaris and Cyprinus carpio) Species Commonly Used in Gambella Region Полный текст
2016
Dagne Tarle | Mitiku Guya | Solomon Abera | getachew bekele fereja
This study was conducted to assess the effect of drying methods and pre-treatments on nutritional content and sensory quality of dried fish. The experiment was conducted in factorial arrangement of 2×3×2 with two drying methods (sun and oven drying), three fish species (tilapia, cat fish and carp) and two preservatives treatment (garlic and ginger juice) laid out in Completely Randomized Design (CRD). Fresh fillets were analysed for their nutritional value and sensory quality. The compositions of the fresh fillets were 6.50-7.59% for ash, 74.20-76.67% for protein, 8.06-9.09% for fat and 8.47-9.12% for total carbohydrates. Drying reduced the moisture contents from 74.74-75.81% to between 7.76-8.25%, making it safe for storage. The ash content changed from 7.11 to 7.34 and from 6.50% to 6.34% for cat fish and tilapia, respectively, with statistical significance whereas no change was observed in carp with 7.60% because of drying. Drying method had no difference in ash and protein contents while increase in fat from 7.75 to 9.44% and a decrease in carbohydrate from 9.37 to 8.13% were observed in sun dried samples than that of oven dried fillets. This study showed that nutritional values of dry fish did not statistically changed during storage period of 3 months.
Показать больше [+] Меньше [-]A Comparative Study Of Probioticated Kunun-Zaki By Probiotic Strains Of Pediococci spp. And Lactobacillus rhamnosus GG Полный текст
2016
Bukola Christianah Adebayo-tayo | Soore Daniel Ademuwagun | Solomon Omoniyi Alao
Non-dairy probiotic functional foods are receiving considerable interest recently owing to the inability to cause allergy in certain segments of the population. Studies on probiotication were carried out to determine the suitability of kunun-zaki as food matrix for production of probiotic beverage using single and mixed culture of Pediococcus acidilactici KU7, Pediococcus pentosaceus CREC5 and Lactobacillus rhamnosus GG. Probioticated and un-probioticated kunun-zaki samples were designated PKZ and UPKZ, respectively. There was a significant difference in the viable count during storage. Viable counts of the LAB strains decreased with increasing storage time with P. acidilactici KU7 having the highest viability at the beginning and end of storage (3.43 ± 0.06 and 0.93± 0.12 x108 cfu/ml). There was a decrease in pH of the PKZ samples within the 1st and 3rd week with corresponding increase in lactic acid production (71.16 ± 0.01 – 441.39 ± 0.01). Marked increase in pH and a decline in lactic acid were observed at the 4th week of storage in all samples. Total soluble solids content of the beverage was observed to decrease with increasing storage time ranging from 8.25 ± 0.01-4.97± 0.01. There was no significant difference in pH and total soluble solids of UPKZ and colour of the PKZ and UPKZ samples during storage. All the PKZ samples were able to inhibit the growth of selected pathogens with marked variability amongst the samples. The UPKZ sample also showed slight inhibitory activity. The samples showed a decline in proximate and mineral composition at the end of storage. Sensory evaluation showed the PKZ samples were preferred over the UPKZ samples. This study shows that probioticated kunun-zaki can serve as a suitable non-dairy alternative to dairy probiotic formulations and would be of health benefits to potential consumers.
Показать больше [+] Меньше [-]Evaluation of Texture Profile, Color and Determination of FOS in Yacón Products (Smallanthus sonchifolius) Полный текст
2016
Valeria Cristina Del Castillo | María Cristina Goldner | Margarita Armada
Textural characteristics, color and fructooligosaccharides (FOS) content, in yacón root products (syrup and dried snack subjected to different pretreatments with NaCl, blanching and ascorbic acid) were evaluated. Yacón from Salta Capital, with 8 months of growth were used. Texture profiles and Color were evaluated instrumentally and FOS content by HPLC. There were significant differences between the samples treated with NaCl and the ones treated by blanching and ascorbic acid for fracture strength, fracture number and hardness according to pretreatment used, and for hardness and tackiness by the drying time. Regarding to color: longer drying time reduces sample brightness. In processed products the FOS content is lower than in fresh yacón, but higher in sucrose, glucose and fructose.
Показать больше [+] Меньше [-]Agricultural Extension Contact and Farmer’s Income in Bangladesh Полный текст
2016
Abu Zafar Mahmudul Haq
The impacts of extension contact on income of farmers have been examined with a view to evaluate the agricultural extension services in Bangladesh. The scope of the study was ten villages of Gazipur of Bangladesh. The objective of the study is to identify the effect of extension contact on crop income, livestock income, fisheries income, miscellaneous income, total agricultural income, nonagricultural income and total income of farmers. The sample of the study consists of 1000 farmers. Data came from field survey and multistage random sampling technique was used for the collection of data. The results indicated that the impacts of extension contact coefficients on crop income, nonagricultural income are positive and significant. However, the impacts of extension contact coefficients are not significant in the cases of livestock, fisheries, miscellaneous and agricultural incomes. But it does not mean that agricultural extension has no reward in Bangladesh. The sample data indicates that the impact of extension contact on total income of farmers is positive and significant. The study concludes that agricultural extension is necessary to raise the income of farmers.
Показать больше [+] Меньше [-]Prevalence of Staphylococcus aureus Isolated From Various Foods of Animal Origin in Kırşehir, Turkey and Their Enterotoxigenicity Полный текст
2016
M. Dilek Avşaroğlu
The aim of this study was to detect Staphylococcus aureus contamination to different types of animal origin foods collected in the Kırşehir province of Turkey and to examine their enterotoxin production ability. Out of 120 food samples 38 suspected colonies were obtained and 23 of them were identified as S. aureus by biochemical and molecular analyses. Other species detected were S. chromogenes, S. cohnii ssp. cohnii, S. hominis, S. lentus, S. warneri, and S. xylosus. The isolates were also analysed with regard to carry mecA gene. None of them was found to have mecA gene indicating susceptibility to methicillin. To determine the enterotoxigenic ability of the isolates phenotypically, reversed-passive-latex-agglutination test against SEA-SED was used. Six out of 23 S. aureus isolates were determined to produce SEA, SEC and SED. Three of them had only one enterotoxin production, whereas others had SEA and SED production together. The results of phenotypic analyses were confirmed by PCR based examination. None of the coagulase-negative staphylococci were found to be enterotoxigenic by both phenotypical and PCR-based analyses. In conclusion, enterotoxigenic S. aureus is a risk in foods of animal origin in Kırşehir and its counties.
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