A study was conducted to determine the effect of De-oiled mahua seed cake (DMSC) on the performance (milk yield, fat percent, nutrient intake, net income and body weight change) of lactating buffaloes (Bubalus bubalis) in Mandla district of Chhattisgarh Hills Region of Central India. Eighteen lactating buffaloes were selected nd divided into three groups (T1, T2 and T3) on the basis of body weight, milk yield, parity and stage of lactation, containing 0, 5 and 10 percent DMSC in replacement of conventional de-oiled seed cakes in the concentrate mixture. The milk yield (kg/day), fat percent, 6 percent FCM yield (kg), DM intake (kg/day) and body weight gain (g/day) was comparable among the groups. However, net income (Rs./animal/day) and B:C ratio was higher in the groups containing DMSC. Thus, from the experiment it was concluded that conventional cake can be replaced with 10 percent DMSC economically without any adverse affect on milk yield, fat percent, nutrient intake and body weight.

This communication reports a case of dystocia due to homozygotic twins suffering from ascites in a primiparous buffalo. Both the dead female fetuses were delivered successfully one by one by manual traction. The dam recovered eventfully without any post obstetrical complications.

A seven years old, ten months pregnant she-buffalo weighing 401 kg was presented to TVCC, COVAS, Udgir with a history of dysphagia, repeated regurgitation and swelling at ventral cervical region since the day before the animal was admitted to the clinic. The animal was suspected for oesophageal obstruction. Endoscopy done by naso-gastric route and X Ray showed oesophageal obstruction with a foreign body situated 100 cm distal from the nasal opening. The animal was cast in right lateral recumbency. The site of incision was shaved, cleaned and scrubbed using liq. 5 percent providine iodine and surgical spirit. The animal was sedated using Xylazine*. Linear infiltration was done using 2 percent lignocaine hydrochloride. A linear incision was taken at the surgical site. The overlying muscles were dissected and the esophagus was exposed. An oesophageal incision of sufficient length was taken and an impacted leather mass was removed. The oesophageal mucosa was closed with simple interrupted suture. Submucosa and muscularis part of esophagus were closed with simple continues manner. The muscles and skin were sutured with simple interrupted sutures. The animal was maintained only on fluid therapy for first 3 days. The day after surgery, the animal was seen lying prostrate with lateral deviation of neck and showing slight vaginal prolapse. The blood sample was analyzed for serum Ca level which revealed a level of 3.9 mg percent indicating severe hypocalcaemia. Calcium therapy was initiated. The animal assumed a standing posture. A day later, the animal delivered a normal female fetus. Three days later serum Ca level was re-estimated to be 8.5 mg percent. The animal resumed normal feeding and rumination after which the animal was given discharge. Sutures were removed after 15 days post operatively. Female buffalo calf and mother buffalo are healthy.

This study was conducted to assess the therapeutic efficiency of antioxidant in subclinical mastitis. Therapeutic trials were conducted in twelve buffaloes affected with subclinical mastitis and were randomly divided into two groups. The buffaloes of Group 1 were treated with intramuscular injection of Inj. Mofoi 5 mg/kg body weight, while Group 2 buffaloes were treated with intramuscular administration of Inj. Mofoi 5 mg/kg body weight along with antioxidant powder orally. The therapeutic efficacy was assessed based on bacteriological cure and the pre and post therapeutic values of somatic cell count, electrical resistance, pH and milk yield. The use of antioxidant along with Moxifloxacin was found to be superior than antibiotic alone by improving udder's natural defence mechanism, early restoration of milk yield and quick recovery.

Present experiment was conducted on sixty Murrah buffalo divided into three groups: lactating, involution stage/dry and pregnant stage (non-lactating early pregnant stage, non-lactating mid pregnant stage and non-lactating late pregnant stage). Most of the alveoli were spherical to oval elongated in shape. In lactating stage, only small and medium sized alveoli were present. In involuting stage due to degeneration and inactiveness only small alveoli were recorded. In non-lactating late pregnant stage most of the alveoli were large sized. A highly significant statistical difference was noted among different stages of lactation in the diameter of alveoli. The active alveoli were lined by cuboidal epithelium to low cuboidal epithelium, while resting alveoli were lined by squamous epithelium with dark nuclei. The number of resting alveoli were found increased with the advancement of lactation. Up to two months of involution most of the alveoli were degenerative. A highly significant statistical difference was noted in the height of alveolar epithelium in different stages of lactation.

Liver infections due to amphistomes in buffaloes cause significant economic losses in livestock sector. The present study investigated the classical morphological features of adult amphistomes combined with histopathology and molecular identity in slaughtered buffaloes from Khyber Pakhtunkhwa, Pakistan. Adult amphistome were collected and morphologically characterized as Gigantocotyle explanatum. Morphometric measurement (n=50) were obtained with 12.05+-1.68 mm in length and 5.77+-0.81 mm in width, and the values of sagittal section was 6.35+-1.03 * 2.98+-0.50 mm in size. Severe bile duct pathology was observed with fibrosis of the duct wall with an irregular epithelial border, hyperplasia and inflammatory response with numerous neutrophils and eosinophils. The molecular identity of G. explanatum within Paramphistomidae was confirmed by ITS-2 rDNA sequences phylogenetic analysis based on maximum likelihood method. The genetic data based on ITS-2 secondary structure of G. explanatum consisted of four helix, Helix I, II and IV were conserved as compared with other closely related reference taxa of family Paramphistomidae and Gastrothylacidae. Helix III expressed some variations. The study concluded that rDNA ITS-2 and secondary structure information provides a guide for other researchers to determine the molecular taxonomic position of Paramphistomidae trematodes, data will support future clinical studies and control measures to reduce the amphistomiasis in buffaloes.

The buffalo rearing is adopting faster rate in arid region of Rajasthan due to its multifaceted utility for both milk and meat. Despite of better management practice are adopted for buffaloes, they also depend upon grazing and poor quality of fodder to meet out their total dry matter intake. The concept of balanced feed with respect to mineral and vitamins are not aware by farmer's community. Thus, the alternative low cost balanced feed was adopted in buffaloes under field condition to augment the milk production with optimum reproductive efficiency. MNFB was developed at feed technology unit of CAZRI, as solidified block of wheat bran, guar meal, dolomite, -minerals and vitamins mixture powder, salt, molasses and urea solution. The study evaluated feeding effect of multi-nutrient feed block on milk production and reproduction performances of lactating buffaloes. The Buffaloes under treatment were divided randomly into two groups and assigned to two dietary treatments under village condition. After calving, all the buffaloes in treatment group were dewormed with albendazole 3 gms and feeding of MNFB 2 blocks (2 kgs each)/week for 3 months during March to May. The control group received diet containing bajara (Pearl millet) kuti, dry grasses, crushed cotton and til (sesame) cake. Milk production, peak milk yield were increased highly significant. Fat percentage was significantly increased. All Observations were monitored as per format. In reproductive performances were like sign of oestrus, conception and post partum reproductive complication 90 percent, 86.6 percent and 0.06 percent in treatment group and 70 percent, 60 percent and 30 percent in Control group respectively. It was concluded that MNFB feeding improved milk yield, milk fat, general health status and reproductive performance of buffalos, as it is a good source of protein, energy, and minerals. It is recommended that MNFB should be used as a supplementation in lactating buffaloes when they area fed with low quality fodder in arid and semi arid region.

The present study was conducted to determine the effect of increasing temperature humidity index (THI) on lactating Murrah buffalo. The study was conducted on six lactating buffaloes which were offered the same basal diet and were blocked by days in milk, milk yield and parity. The study was conducted during May, June and July with average THI of 79.88, 80.57 and 85.36, respectively. Milk yield was recorded twice a day at 06:00 and 18:00 h and physiological parameters were recorded and blood was sampled on 30th day of each month at 14:30 h. The results showed a significant decrease (P LT 0.05) in milk yield in buffaloes only at a THI of 85.36 as compared to THI up to 80.57. The rectal temperature (RT) did not change (P=0.580) with increasing THI up to 85.36, however, the respiration rate (RR) and pulse rate (PR) changed (P LT 0.05) progressively with increasing THI. Leucocytopnoea, lymphocytopnoea and neutrophilia; and alteration in serum urea, alkaline phosphatase activity, reactive oxygen species and phosphorus concentration were observed only at a THI of 85.36 as compared to THI up to 80.57. Similarly, significant (P LT 0.05) alteration in serum T4, cortisol and prolactin levels were observed at a THI of 85.36 as compared to THI up to 80.57. It can be concluded that lactating buffaloes experienced mild heat stress up to a THI of 80.57 (May and June) and a moderate type of heat stress at a THI 85.36 (July).

The present study was conducted to identify specific acoustic features which could be used as possible indicators for early diagnosis of pneumonia in buffalo calves. In pneumonia, change in elasticity and resonance of vocal sound producing organs occur which eventually affects the vocal signature of calves. Twenty Murrah buffalo calves' voice was recorded during both healthy and pneumonia infected stage where pneumonia was confirmed by lung X-RAY radiography. From the recorded vocal sound, acoustic features viz. call duration (sec.), call interval (sec.), frequency (Hz), bandwidth (Hz) and peak amplitude (P) with their sub variants were extracted with the help of PRAAT 3.2.36 software. Out of these, call duration (sec.) (0.879+-0.29 v/s 0.689+-0.24), call interval (sec.) (0.288+-0.059 v/s 0.107+-0.047) and peak amplitude (P) (start (0.750+-0.118 v/s 0.435+-0.113), end (0.102+-0.045 v/s 0.508+-0.268) and maximum (0.938+-0.210 v/s 0.684+-0.480)) were found significantly (p LT 0.05) different between two groups. Rest acoustic features did not differ statistically between two groups. This study indicates that it is possible to discriminate pneumonia voice from normal/healthy voice by acoustic analysis and farmers can acquire an early warning of pneumonia infections in calves through this non-invasive method.

Cryopreservation is connected with increased undesirable premature sperm capacitation, reduced lifespan and fertility results. Taurine acts as an antioxidant improving the semen quality after cryopreservation. The objective of the current study is to assess the effect of addition of different levels of taurine amino acid to tris-extender on bull sperm cryo-preservability. Methods: The collected semen samples were pooled and diluted with tris-citric acid-fructose egg yolk extender (control, 0 percent taurine) and different concentrations of taurine (10 mM, 20 Mm, 30 Mm, 40 Mm, 50 Mm, 60 Mm, 70 Mm, 80 Mm, 90 Mm and 100 mM) to final concentration of 60 *10**(6) motile sperm/ml. Diluted semen was processed for freezing and evaluated for sperm parameters. Results: Data showed that taurine enriched extender improved sperm parameters during cooling. Post thawing sperm motility percent, membrane integrity percent, Alive sperm percent improved and the percentages of abnormal sperm was lowered upon using taurine enriched extender. Conclusion: It could be concluded that, most the concentrations of taurine in Tris-citrate egg yolk extender ameliorated the post cooling and post freezing semen quality in buffalo bulls.