ABSTRACT In the present study, we evaluated the influence of agroforestry systems and traditional agriculture on the physical attributes and biologicals of soil, using as reference a native forest area in Paraty, RJ. Levels of total soil organic carbon, as well as oxidizable fractions and aggregate stability, were analyzed, in addition to the activity and carbon and nitrogen content in the soil microbial biomass, in the 0-5 cm layer, in two distinct seasons (dry and rainy seasons). The agroforestry systems and traditional agriculture maintain high levels of total organic carbon and its storage in more stable fractions. These systems provide the same conditions as those of the forest area for the aggregation of the soil. The microbial biomass was more responsive to variations in management and seasonality. The deployment of agroforestry systems contributes to the formation of a more diversified agricultural matrix, promoting improvements in the soil quality and connection with the remaining adjacent forest. | RESUMO Esse estudo avaliou a influência dos sistemas agroflorestais e da agricultura tradicional sobre os atributos físicos e biológicos do solo, utilizando como referência uma área de floresta nativa em Paraty, RJ. Foram analisados os teores do carbono orgânico total do solo e suas frações oxidáveis, a estabilidade dos agregados, além da atividade e os teores de carbono e nitrogênio da biomassa microbiana do solo, na camada de 0-5 cm, em duas épocas (seca e chuvosa) distintas. Os sistemas agroflorestais e a agricultura tradicional mantêm níveis elevados de carbono orgânico total e seu armazenamento em frações mais estáveis. Quando comparados à área de Floresta, esses sistemas proporcionam as mesmas condições para a agregação do solo. A biomassa microbiana foi mais responsiva as variações de manejo e sazonalidade. A implantação de sistemas agroflorestais contribui para a formação de uma matriz agrícola mais diversificada, promovendo melhorias na qualidade do solo e conexão com os remanescentes florestais próximos.

In the present study, we evaluated the influence of agroforestry systems and traditional agriculture on the physical attributes and biologicals of soil, using as reference a native forest area in Paraty, RJ. Levels of total soil organic carbon, as well as oxidizable fractions and aggregate stability, were analyzed, in addition to the activity and carbon and nitrogen content in the soil microbial biomass, in the 0-5 cm layer, in two distinct seasons (dry and rainy seasons). The agroforestry systems and traditional agriculture maintain high levels of total organic carbon and its storage in more stable fractions. These systems provide the same conditions as those of the forest area for the aggregation of the soil. The microbial biomass was more responsive to variations in management and seasonality. The deployment of agroforestry systems contributes to the formation of a more diversified agricultural matrix, promoting improvements in the soil quality and connection with the remaining adjacent forest.

ABSTRACT Biochar is a solid material formed during biomass thermochemical decomposition processes. This organic compound has particular properties that may cause effects on soils depending on its feedstock and processing conditions. Thus, the characteristics and purpose of use of this material must be recognized prior to its use. Two types of biochar, derived from different wood sources, were compared, one from caatinga biome species and another from cashew trees. Two species from caatinga biome were used, jurema-preta (Mimosa tenuiflora Willd. Poir.), and marmeleiro (Croton sonderianus Müll. Arg.). This study aimed to identify the best biochar material regionally available to increase water-holding capacity in the soil, based on laboratory tests and microstructural porosity evaluation. Biochar from Caatinga wood demonstrated an improved water-holding capacity if compared to cashew wood biochar. The particle diameters of 2 and 4 mm showed the highest levels, which were 2,268 g.g-1 for caatinga wood and 0.574 g.g-1 for cashew wood biochars, respectively. While the smaller quantities of macropores and a larger number of micropores (smaller radius) could explain the higher water-holding capacity for biochar from caatinga wood, the thick lignified cell walls of biochar from cashew wood support the idea of a hydrophobic effect contributing to water lower holding capacity. | RESUMO Biocarvão é o material sólido formado durante a decomposição termoquímica da biomassa. Suas propriedades e efeitos no solo variam amplamente com o material de origem e condições de precessamento. Desta forma, é reconhecida a importância de sua caracterização e desempenho para o que se propõe, antes do uso. Foram comparados dois tipos de biochar, de acordo com o material de origem, espécies do bioma Caatinga, Jurema-Preta (Mimosa tenuiflora (Willd.) Poir. associada com Marmeleiro (Croton sonderianus Müll.) Arg. e lenha de cajueiro (Annacardium occidentale L.). O objetivo deste estudo foi identificar biocarvão com melhor desempenho de promover o aumento da capacidade de retenção de água no solo, com base em evidências de laboratório e avaliação de porosidade microestructural. O biocarvão oriundo das espécies da Caatinga demonstrou maior capacidade de retenção de água quando comparado com o biocarvão de lenha de cajueiro, para todos os diâmetros avaliados. Com relação ao tamanho de partículas, níveis maiores de retenção de água foram observados aos 2 mm e 4 mm, 2,268 g g-1 para o biocarvão de madeira das espécies da Caatinga e 0,574 g g-1 para o biocarvão de madeira de cajueiro, respectivamente. Menores quantidades de macroporos e maior número de microporos (raio menor) foram observados no biocarvão de madeira das espécies da Caatinga, provavelmente relacionado à mais alta capacidade de retenção de água, enquanto densamente lignificadas, as paredes celulares de madeira de cajueiro, sustenta a ideia de que um efeito hidrofóbico pode contribuir para sua menor capacidade de retenção hídrica.

Biochar is a solid material formed during biomass thermochemical decomposition processes. This organic compound has particular properties that may cause effects on soils depending on its feedstock and processing conditions. Thus, the characteristics and purpose of use of this material must be recognized prior to its use. Two types of biochar, derived from different wood sources, were compared, one from caatinga biome species and another from cashew trees. Two species from caatinga biome were used, jurema-preta (Mimosa tenuiflora Willd. Poir.), and marmeleiro (Croton sonderianus Müll. Arg.). This study aimed to identify the best biochar material regionally available to increase water-holding capacity in the soil, based on laboratory tests and microstructural porosity evaluation. Biochar from Caatinga wood demonstrated an improved water-holding capacity if compared to cashew wood biochar. The particle diameters of 2 and 4 mm showed the highest levels, which were 2,268 g.g-1 for caatinga wood and 0.574 g.g-1 for cashew wood biochars, respectively. While the smaller quantities of macropores and a larger number of micropores (smaller radius) could explain the higher water-holding capacity for biochar from caatinga wood, the thick lignified cell walls of biochar from cashew wood support the idea of a hydrophobic effect contributing to water lower holding capacity.

ABSTRACT Drip irrigation with diluted sanitary landfill leachate favors the development of grasses on the landfill surface, however, the clogging of emitters of the irrigation systems is the major obstacle for this practice. The objective of this study was to select empirical models of the Christiansen's uniformity coefficient, distribution uniformity coefficient, and statistical uniformity coefficient of drip irrigation sets applying sanitary landfill leachate diluted in water as a function of time of operation, and quality of the effluent. An experiment was conducted in a completely randomized experimental design with three replicates, using a split-split plot arrangement, with operating pressures (70, 140, 210, and 280 kPa) in the plots, types of drippers (G1, 1.65 L h-1; G2, 2.0 L h-1; and G3, 4.0 L h-1) in the subplots, and operating time of the drip irrigation sets (0, 20, 40, 60, 80, 100, 120, 140, and 160 hours) in the sub-subplots. Data regarding hydraulic performance and effluent quality were acquired every 20 hours during 160 hours of operation and subjected to simple and multiple regression analyses. The G3 dripper operating at pressures of 70 and 140 kPa were the combinations that best attenuated biofilm clogging. The linear regression and square root were the models that best represented the reduction in the uniformity of the effluent application as a function of the operating time of the drip irrigation sets. The linear correlations denoted that calcium contents and total coliform population rates were the characteristics of the sanitary landfill leachate that most affected the drip clogging process. | RESUMO A aplicação localizada de percolado diluído permite o desenvolvimento de gramíneas na superfície das células dos aterros sanitários, entretanto a obstrução dos emissores dos sistemas de irrigação consiste no maior obstáculo desta atividade. Objetivou-se com a realização do presente trabalho selecionar modelos empíricos do coeficiente de uniformidade de Christiansen, coeficiente de uniformidade de distribuição e coeficiente de uniformidade estatístico de unidades gotejadoras aplicando percolado diluído em função do tempo de operação e da qualidade do efluente. Para isso, montou-se um experimento em parcelas subsubdivididas. Nas parcelas tem-se as pressões de serviço (70, 140, 210 e 280 kPa), nas subparcelas os tipos de gotejadores (G1 - 1,65 L h-1; G2 - 2,0 L h-1; e 4,0 L h-1) e nas subsubparcelas os tempos de operação das unidades gotejadoras (0, 20, 40, 60, 80, 100, 120, 140 e 160 h). O delineamento experimental foi o inteiramente casualizado com três repetições. Os dados dos indicadores de desempenho hidráulico e da qualidade do efluente foram obtidos a cada 20 h, até completar 160 h de funcionamento, sendo posteriormente submetidos às análises de regressão simples e múltipla. Os resultados indicaram que o gotejador G3 operando nas pressões de serviço 70 e 140 kPa foram as combinações que melhor atenuaram o entupimento com biofilme. Os modelos de regressão linear e raiz quadrada foram os que melhor representaram a redução da uniformidade de aplicação do efluente em função do tempo de operação nas unidades gotejadoras. As relações lineares revelaram que os teores de cálcio e os níveis populacionais de coliformes totais foram as características do percolado de aterro diluído que mais interferiram no processo de obstrução dos gotejadores.

Drip irrigation with diluted sanitary landfill leachate favors the development of grasses on the landfill surface, however, the clogging of emitters of the irrigation systems is the major obstacle for this practice. The objective of this study was to select empirical models of the Christiansen's uniformity coefficient, distribution uniformity coefficient, and statistical uniformity coefficient of drip irrigation sets applying sanitary landfill leachate diluted in water as a function of time of operation, and quality of the effluent. An experiment was conducted in a completely randomized experimental design with three replicates, using a split-split plot arrangement, with operating pressures (70, 140, 210, and 280 kPa) in the plots, types of drippers (G1, 1.65 L h-1; G2, 2.0 L h-1; and G3, 4.0 L h-1) in the subplots, and operating time of the drip irrigation sets (0, 20, 40, 60, 80, 100, 120, 140, and 160 hours) in the sub-subplots. Data regarding hydraulic performance and effluent quality were acquired every 20 hours during 160 hours of operation and subjected to simple and multiple regression analyses. The G3 dripper operating at pressures of 70 and 140 kPa were the combinations that best attenuated biofilm clogging. The linear regression and square root were the models that best represented the reduction in the uniformity of the effluent application as a function of the operating time of the drip irrigation sets. The linear correlations denoted that calcium contents and total coliform population rates were the characteristics of the sanitary landfill leachate that most affected the drip clogging process.

RESUMO O semiárido representa cerca de 70% da superfície do Nordeste brasileiro e o recurso forrageiro de maior expressão é a Caatinga, cobrindo aproximadamente 54% desta região. Porém, cerca de 40% dessa vegetação encontra-se em sucessão secundária e as áreas em processo de degradação de intensidade baixa a severa, já somam mais de 20 milhões de hectares, reflexo do uso intenso da terra, dentre elas o superpastejo. Objetivou-se avaliar a influência de diferentes doses de fósforo (0, 50 e 100 kg de P2O5 ha-1 ano), em área de Caatinga manipulada, sem pastejo por quatro anos, sobre a dinâmica da vegetação herbácea em diferentes épocas do ano. O experimento foi conduzido na Unidade Acadêmica de Serra Talhada-UFRPE, durante os anos de 2015 a 2016. Foi utilizado delineamento em blocos casualizados, com três blocos e quatro repetições por bloco. A adubação fosfatada em áreas de exclusão ao pastejo promoveu aumento da massa de forragem do estrato herbáceo da Caatinga enriquecida, em todas as épocas de avaliação, com destaque para a época seca de 2016, que obeteve valores acima da média de 7950 kg de MS ha-1. Promoveu aumento da participação do capim-buffel na composição florística, elevando sua participação na área em torno de 74% para a dose máxima de fósforo utilizada. Neste sentido, a adubação fosfatada até 100 kg de P2O5 ha-1 ano, juntamente com a exclusão ao pastejo, pode ajudar na recuperação da Caatinga enriquecida com capim-buffel e otimizar a sua utilização diminuindo a formação de novas áreas de pastejo na Caatinga. | ABSTRACT The semi-arid region accounts for about 70% of the surface area of the Brazilian northeast, and the most important forage resource is the Caatinga, covering approximately 54% of this region. However, about 40% of this vegetation is in secondary succession. The areas in the process of degradation range from low to severe intensity, and total more than 20 million hectares, reflecting the intense use of the land, such as the overgrazing. The objective of this study was to evaluate the influence of different doses of phosphorus (0, 50 and 100 kg P2O5 ha-1 year-1) on the dynamics of herbaceous vegetation at different times of the year, in a Caatinga area manipulated without grazing for four years. The experiment was conducted at the Serra Talhada-UFRPE Academic Unit during the years 2015 and 2016. We used a randomised block design with three blocks and four replicates per block. Phosphate fertilisation in grazing exclusion areas promoted an increase in forage mass of the enriched Caatinga herbaceous stratum at all evaluation times, especially in the dry season of 2016, which showed values above the average of 7950 kg DM ha-1. In addition, it promoted an increase in the participation of buffelgrass in the floristic composition, increasing its involvement in the area to around 74% at the maximum dose of phosphorus used. Thus, phosphate fertilisation at up to 100 kg of P2O5 ha-1 year-1, together with grazing exclusion, can help to recover the Caatinga enriched with buffelgrass and optimise its use by reducing the formation of new grazing areas in the Caatinga.

The semi-arid region accounts for about 70% of the surface area of the Brazilian northeast, and the most important forage resource is the Caatinga, covering approximately 54% of this region. However, about 40% of this vegetation is in secondary succession. The areas in the process of degradation range from low to severe intensity, and total more than 20 million hectares, reflecting the intense use of the land, such as the overgrazing. The objective of this study was to evaluate the influence of different doses of phosphorus (0, 50 and 100 kg P2O5 ha-1 year-1) on the dynamics of herbaceous vegetation at different times of the year, in a Caatinga area manipulated without grazing for four years. The experiment was conducted at the Serra Talhada-UFRPE Academic Unit during the years 2015 and 2016. We used a randomised block design with three blocks and four replicates per block. Phosphate fertilisation in grazing exclusion areas promoted an increase in forage mass of the enriched Caatinga herbaceous stratum at all evaluation times, especially in the dry season of 2016, which showed values above the average of 7950 kg DM ha-1. In addition, it promoted an increase in the participation of buffelgrass in the floristic composition, increasing its involvement in the area to around 74% at the maximum dose of phosphorus used. Thus, phosphate fertilisation at up to 100 kg of P2O5 ha-1 year-1, together with grazing exclusion, can help to recover the Caatinga enriched with buffelgrass and optimise its use by reducing the formation of new grazing areas in the Caatinga.

RESUMO O feijão comum (Phaseolus vulgaris L.) é uma das fontes proteicas mais importantes em todo o mundo. Todavia, o ataque de bruquídeos inviabiliza o armazenamento do produto após a colheita. O objetivo deste trabalho foi determinar a taxa de emergência de Zabrotes subfasciatus (Coleoptera: Chrysomelidae) em quatro variedades de feijão P. vulgaris (Carioca Pitoco, Enxofre, Gorgutuba Vermelho e Rosinha) cultivadas na Amazônia sul-ocidental (Acre, Brasil). Os grãos de cada variedade foram infestados com 50 adultos não-sexados e após 13 dias do início dos bioensaios, os insetos foram removidos. A progênie adulta obtida nas variedades foi contabilizada e removida em dias alternados, a partir da primeira emergência, até o final do período de emergência (n° de insetos/frasco). A soma da emergência de insetos foi acumulada a partir da emergência inicial e resultou na soma da emergência acumulada (SEa, % dia), calculada por SEa = ∑SEd. Foram constadas diferenças nas taxas de emergência de Z. subfasciatus entre as variedades de feijão, sendo que a variedade Gorgutuba Vermelho apresentou menor taxa de emergência. Embora esta variedade de feijão tenha apresentado menor emergência de adultos, o tempo de desenvolvimento dos insetos não se estendeu nesta variedade, em comparação com as outras variedades. Em geral, a variedade Gorgutuba Vermelho apresentou indícios de fontes resistência a Z. subfasciatus. | ABSTRACT Common beans (Phaseolus vulgaris L.) are one of the most important protein sources worldwide. However, infestation by bruchids compromises the storage of these beans after harvest. The objective of this study was to determine the emergence rate of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in four P. vulgaris varieties (Carioca Pitoco, Enxofre, Gorgutuba Vermelho, and Rosinha) cultivated in the southwestern Amazon (State of Acre, Brazil). The grains from each variety were infested with 50 non-sexed adult Z. subfasciatus, and the insects were collected 13 days after initiating the bioassays. The adult progeny was collected from each plant variety on alternate days from the beginning to the end of the emergence period, and they were counted (number of insects/jar). The sum of emerged insects per day (SEd) was determined from the beginning of the emergence period, from which the accumulated emergence was calculated (SEa= ΣSEd, % day). There were differences in the emergence rates of Z. subfasciatus among the bean varieties, with the rates being lower in the Gorgutuba Vermelho variety. Although this bean variety had the lowest peak of adult insect emergence, the period of insect development was not of increased length in this variety compared to the other varieties. In general, the Gorgutuba Vermelho variety appears to be resistant to Z. subfasciatus.

Common beans (Phaseolus vulgaris L.) are one of the most important protein sources worldwide. However, infestation by bruchids compromises the storage of these beans after harvest. The objective of this study was to determine the emergence rate of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in four P. vulgaris varieties (Carioca Pitoco, Enxofre, Gorgutuba Vermelho, and Rosinha) cultivated in the southwestern Amazon (State of Acre, Brazil). The grains from each variety were infested with 50 non-sexed adult Z. subfasciatus, and the insects were collected 13 days after initiating the bioassays. The adult progeny was collected from each plant variety on alternate days from the beginning to the end of the emergence period, and they were counted (number of insects/jar). The sum of emerged insects per day (SEd) was determined from the beginning of the emergence period, from which the accumulated emergence was calculated (SEa= ΣSEd, % day). There were differences in the emergence rates of Z. subfasciatus among the bean varieties, with the rates being lower in the Gorgutuba Vermelho variety. Although this bean variety had the lowest peak of adult insect emergence, the period of insect development was not of increased length in this variety compared to the other varieties. In general, the Gorgutuba Vermelho variety appears to be resistant to Z. subfasciatus.

RESUMO O objetivo deste trabalho foi avaliar a eficiência, fitotoxicidade e efeito residual do extrato aquoso de folhas de Prosopis juliflora (Sw.) DC. (Fabaceae) para Tetranychus bastosi Tuttle, Baker & Sales (Acari: Tetranychidae) em Jatropha curcas L. (Euphorbiaceae). Para a avaliação da eficiência de controle plantas de pinhão-manso foram infestadas com 30 fêmeas adultas do ácaro e após 12 dias, foram pulverizadas com as concentrações letais (m/v) do extrato (CL50 = 53,45% ou CL90 = 85,35%) e com água destilada (testemunha), correspondendo aos tratamentos. Decorridas 24, 48, 72 e 96 e 120 horas após a aplicação do extrato, duas folhas do terço inferior, médio e superior das plantas foram amostradas e contabilizou-se os ácaros vivos por tratamento. A avaliação do efeito residual foi feita três, 24, 48, 96, 192 e 288 horas após a pulverização. Para avaliação do efeito fitotóxico do extrato foram atribuídas notas de acordo com a intensidade dos sintomas nas plantas. Constatou-se eficiência de controle por todo período avaliado, com média de 81,67% para a CL50 e 73,05% para CL90, sem diferença significativa entre os intervalos de avaliação. O extrato apresentou baixo efeito residual sobre T. bastosi, mas ao final de 12 dias a porcentagem média de redução da oviposição do ácaro foi de 49,21% e 68,86% para a CL50 e CL90, respectivamente. As plantas não apresentaram fitotoxicidade. O extrato de algarobeira apresenta potencial para o controle alternativo de T. bastosi em pinhão-manso em razão de sua eficiência sobre a mortalidade deste ácaro, redução da oviposição de fêmeas e ausência de efeito fitotóxico nas plantas. | ABSTRACT The objective of this study was to evaluate the efficiency, phytotoxicity and residual effect of the aqueous extract of leaves of Prosopis juliflora (Sw.) DC (Fabaceae) for Tetranychus bastosi Tuttle, Baker & Sales (Acari: Tetranychidae) on Jatropha curcas L. (Euphorbiaceae). For the evaluation of the control efficiency, physic nut plants were infested with 30 adult mite females and after 12 days were sprayed with the lethal concentrations (m/v) of the extract (LC50 = 53.45% or CL90 = 85.35%) and with distilled water (control), which corresponded to the treatments. After 24, 48, 72, 96 and 120 hours after application of the extract, two leaves of the lower, middle and upper third of the plants were sampled and the live mites were counted per treatment. The evaluation of the residual effect was done three, 24, 48, 96, 192 and 288 hours after spraying. To evaluate the phytotoxic effect of the extract, scores were given according to the intensity of the symptoms in the plants. Control efficiency was verified throughout the evaluated period, with an average of 81.67% for LC50 and 73.05% for LC90, with no significant difference between the evaluation intervals. The extract had a low residual effect on T. bastosi, but at the end of 12 days the average percentage of oviposition reduction of mite was 49.21% and 68.86% for LC50 and LC90, respectively. The plants did not present phytotoxicity. P. juliflora extract presents potential for the alternative control of T. bastosi in physic nut due to its efficiency on the mortality of this mite, oviposition reduction of females and absence of phytotoxic effect in the plants.

The objective of this study was to evaluate the efficiency, phytotoxicity and residual effect of the aqueous extract of leaves of Prosopis juliflora (Sw.) DC (Fabaceae) for Tetranychus bastosi Tuttle, Baker & Sales (Acari: Tetranychidae) on Jatropha curcas L. (Euphorbiaceae). For the evaluation of the control efficiency, physic nut plants were infested with 30 adult mite females and after 12 days were sprayed with the lethal concentrations (m/v) of the extract (LC50 = 53.45% or CL90 = 85.35%) and with distilled water (control), which corresponded to the treatments. After 24, 48, 72, 96 and 120 hours after application of the extract, two leaves of the lower, middle and upper third of the plants were sampled and the live mites were counted per treatment. The evaluation of the residual effect was done three, 24, 48, 96, 192 and 288 hours after spraying. To evaluate the phytotoxic effect of the extract, scores were given according to the intensity of the symptoms in the plants. Control efficiency was verified throughout the evaluated period, with an average of 81.67% for LC50 and 73.05% for LC90, with no significant difference between the evaluation intervals. The extract had a low residual effect on T. bastosi, but at the end of 12 days the average percentage of oviposition reduction of mite was 49.21% and 68.86% for LC50 and LC90, respectively. The plants did not present phytotoxicity. P. juliflora extract presents potential for the alternative control of T. bastosi in physic nut due to its efficiency on the mortality of this mite, oviposition reduction of females and absence of phytotoxic effect in the plants.

Flour production through milling is an alternative for the cowpea processing, when the aim is to obtain a product with higher added value. The objective of this work was to determine the chemical composition, the granulometry, and evaluate the microbiological characteristics of whole-bean flours from five cowpea cultivars (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera and BRS Itaim). Cowpea beans were dried in a oven (Fanem, 320-SE) at 50 °C for 6 hours, milled in a cyclone-type rotor mill (Tecnal, TE-651/2) and, subsequently, in a pulverizer-type semi-industrial mill (Fritsch, Pulverisette 14) to obtain the flour. The flour centesimal composition, total energetic value, mineral content, granulometry, microbiological characteristics, and technological properties-flour water absorption and solubility in water-were analyzed. All analyzes were performed in triplicate and the results expressed in mean ± standard deviation. The means were compared by the Scott-Knott test (p ≤ 0.05). The flour centesimal composition presented (g per 100 g of flour): 7.13 to 10.33 of moisture, 2.06 to 3.65 of ashes, 1.53 g to 2.90 of lipids, 21.73 to 25.77 of proteins, and 58.08 to 64.02 of carbohydrate. The flours had on average 359.04 Kcal (100 g) and presented high iron, zinc, copper, manganese, phosphorus and magnesium contents. The whole-bean flours from the cultivars BRS Cauamé and BRS Xiquexique are sources of calcium. Regarding the microbiological characteristics, all flours are innocuous. The water absorption index were within 1.77 and 2.20 g g-1 and the water solubility index within 17 and 23%. All whole-bean flours had good nutritional, technological and microbiological characteristics, and thus potential for use in bakery products.

ABSTRACT Flour production through milling is an alternative for the cowpea processing, when the aim is to obtain a product with higher added value. The objective of this work was to determine the chemical composition, the granulometry, and evaluate the microbiological characteristics of whole-bean flours from five cowpea cultivars (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera and BRS Itaim). Cowpea beans were dried in a oven (Fanem, 320-SE) at 50 °C for 6 hours, milled in a cyclone-type rotor mill (Tecnal, TE-651/2) and, subsequently, in a pulverizer-type semi-industrial mill (Fritsch, Pulverisette 14) to obtain the flour. The flour centesimal composition, total energetic value, mineral content, granulometry, microbiological characteristics, and technological properties-flour water absorption and solubility in water-were analyzed. All analyzes were performed in triplicate and the results expressed in mean ± standard deviation. The means were compared by the Scott-Knott test (p ≤ 0.05). The flour centesimal composition presented (g per 100 g of flour): 7.13 to 10.33 of moisture, 2.06 to 3.65 of ashes, 1.53 g to 2.90 of lipids, 21.73 to 25.77 of proteins, and 58.08 to 64.02 of carbohydrate. The flours had on average 359.04 Kcal (100 g) and presented high iron, zinc, copper, manganese, phosphorus and magnesium contents. The whole-bean flours from the cultivars BRS Cauamé and BRS Xiquexique are sources of calcium. Regarding the microbiological characteristics, all flours are innocuous. The water absorption index were within 1.77 and 2.20 g g-1 and the water solubility index within 17 and 23%. All whole-bean flours had good nutritional, technological and microbiological characteristics, and thus potential for use in bakery products. | RESUMO A produção de farinha por meio da moagem é uma alternativa para o processamento do feijão-caupi, na busca de um produto com maior valor agregado. Objetivou-se determinar a composição química, a granulometria e avaliar microbiologicamente farinhas integrais de cinco cultivares (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera e BRS Itaim) comerciais de feijão-caupi. Para a obtenção das farinhas procedeu-se a secagem dos grãos em estufa (marca Fanem, modelo 320-SE) a 50 °C, por 6 horas; com posterior moagem, primeiramente em moinho de facas tipo ciclone (marca Tecnal, modelo TE-651/2) e posteriormente, em moinho semi-industrial tipo pulverizador (marca Fritsch, modelo Pulverisette 14). Analisaram-se a composição centesimal, o valor energético total, o conteúdo mineral, a granulometria, as características microbiológicas, e as tecnológicas quanto a absorção e solubilidade em água das farinhas. As análises foram realizadas em triplicata e os resultados expressos em média ± desvio padrão. As médias foram comparadas pelo teste de Scott-Knott (p≤0,05). Os valores encontrados para composição centesimal foram: umidade entre 7,13 a 10,33 g/100g; cinzas de 2,06 a 3,65 g/100g; lipídios variaram de 1,53 g a 2,90 g/100g; proteínas entre 21,73 a 25,77 g/100g e o conteúdo de carboidratos variou de 58,08 a 64,02 g/100g. As farinhas ofertaram em média 359,04 Kcal/100g e apresentaram elevado teor de ferro, zinco, cobre, manganês, fósforo e magnésio, sendo as das cultivares BRS Cauamé e BRS Xiquexique fontes de cálcio. Sob o aspecto microbiológico as farinhas apresentaram-se inócuas. Os valores de IAA variaram de 1,77 a 2,20 g/g e ISA de 17 a 23%. Concluiu-se que todas as farinhas apresentaram bom perfil nutritivo, tecnológico e microbiológico, apresentando, portanto potencial para utilização no desenvolvimento de produtos de panificação.

The objective of the present study was to evaluate the tolerance to cadmium (Cd) of sunflower genotypes grown in greenhouse conditions, and the effectiveness of using photosynthetic parameters as physiological indicators of this tolerance. Seeds of two sunflower genotypes previously identified as tolerant (H358) and Cd-sensitive (AG960) to Cd were used. The seeds were germinated in plastic cups containing plant substrate; after 9 days, the seedlings were transplanted to plastic basins containing a nutrient solution with 0 or 10 µM of Cd, where they remained for 16 days. Samples of the plants were harvested every 5 days. The experiment was carried out in a randomized complete design, using a 4×2×2 factorial arrangement (4 days of grown in a nutrient solution with Cd, 2 sunflower genotypes, and 2 Cd levels) with four replications. Cd stress decreased CO2 net assimilation, stomatal conductance, carboxylation efficiency, photosynthetic pigment contents, potential quantum yield (Fv/Fm), and effective quantum yield of plants of the two evaluated genotypes. The decrease in photosynthetic rates of these plants was caused by both stomatal and non-stomatal limitations. Plants of the AG960 genotype showed more pronounced deleterious effects due to Cd stress than those of the H358 genotype. Thus, CO2 net assimilation rate, stomatal conductance, and chloroplast pigment content are good physiological indicators of sunflower tolerance to Cd and can at least in part, explain the greater tolerance of the H358 genotype to Cd stress when compared to the AG960 genotype.

ABSTRACT The objective of the present study was to evaluate the tolerance to cadmium (Cd) of sunflower genotypes grown in greenhouse conditions, and the effectiveness of using photosynthetic parameters as physiological indicators of this tolerance. Seeds of two sunflower genotypes previously identified as tolerant (H358) and Cd-sensitive (AG960) to Cd were used. The seeds were germinated in plastic cups containing plant substrate; after 9 days, the seedlings were transplanted to plastic basins containing a nutrient solution with 0 or 10 µM of Cd, where they remained for 16 days. Samples of the plants were harvested every 5 days. The experiment was carried out in a randomized complete design, using a 4×2×2 factorial arrangement (4 days of grown in a nutrient solution with Cd, 2 sunflower genotypes, and 2 Cd levels) with four replications. Cd stress decreased CO2 net assimilation, stomatal conductance, carboxylation efficiency, photosynthetic pigment contents, potential quantum yield (Fv/Fm), and effective quantum yield of plants of the two evaluated genotypes. The decrease in photosynthetic rates of these plants was caused by both stomatal and non-stomatal limitations. Plants of the AG960 genotype showed more pronounced deleterious effects due to Cd stress than those of the H358 genotype. Thus, CO2 net assimilation rate, stomatal conductance, and chloroplast pigment content are good physiological indicators of sunflower tolerance to Cd and can at least in part, explain the greater tolerance of the H358 genotype to Cd stress when compared to the AG960 genotype. | RESUMO O presente estudo foi conduzido em casa de vegetação objetivando-se demonstrar a tolerância ao cádmio (Cd) em genótipos de girassol e avaliar a eficácia dos parâmetros fotossintéticos como indicadores fisiológicos dessa tolerância. Para isto, foram utilizadas sementes de dois genótipos de girassol previamente identificados como tolerante (H358) e sensível (AG960) ao Cd. As sementes foram germinadas em copos plásticos contendo substrato vegetal e, após 9 dias, as plântulas foram transferidas para bacias plásticas com solução nutritiva contendo 0 ou 10 µM de Cd , onde permaneceram por 16 dias. As plantas foram coletadas a cada 5 dias. O delineamento experimental foi o inteiramente casualizado, em um arranjo fatorial 4 (dias de exposição) × 2 (genótipos) × 2 (níveis de Cd), com quatro repetições. A exposição ao Cd reduziu a taxa de assimilação líquida do CO2, a condutância estomática e a eficiência de carboxilação, bem como os teores de todos os pigmentos fotossintéticos, a razão Fy/Fm e o YII das plantas de ambos os genótipos estudados. Demonstrando que a redução na taxa fotossintética dessas plantas foi ocasionada tanto por limitações estomáticas como não-estomáticas. Os efeitos deletérios do Cd foram mais pronunciados no genótipo AG960 em comparação com o H358. Assim, as variáveis A, gs e os teores de pigmentos mostraram-se bons indicadores fisiológicos da tolerância do girassol ao estresse por Cd e podem, ao menos em parte, explicar a maior tolerância do genótipo H358 em relação ao AG960.

The Brazilian bocachico, Prochilodus brevis, is a rheophilic fish. Although there is evidence that this species shows reproductive seasonality in the wild, in captivity hormonal induction techniques allow semen sampling in different seasons. This study aimed to compare the kinetics, morphology and biochemical composition of the semen of Brazilian bocachico in captivity when hormonally induced to breed in the reproductive and non-reproductive seasons. During sampling spermiation was hormonally induced in breeders. The concentrations of total protein, glucose, fructose, triglyceride, calcium and chloride were evaluated with biochemical kits. The pH data (6.5 to 8.5) suggest semen requires alkaline conditions, as expected for freshwater fish. Seminal plasma contained more protein (1.51 ± 0.06 dL g-1), glucose (79.44 ± 1.88 mg dL-1) and triglycerides (61.59 ± 8.10 mg dL-1) in the non-reproductive than the reproductive season, but calcium ions (15.98 ± 1.02 mg dL-1) showed the opposite pattern. There was a significant seasonal difference in sperm morphology, with a higher percentage of normal sperm in the reproductive season. From these data it can be concluded that the physical, kinetic, morphological and biochemical characteristics of semen of captive Prochilodus brevis are influenced by reproductive season.

ABSTRACT The Brazilian bocachico, Prochilodus brevis, is a rheophilic fish. Although there is evidence that this species shows reproductive seasonality in the wild, in captivity hormonal induction techniques allow semen sampling in different seasons. This study aimed to compare the kinetics, morphology and biochemical composition of the semen of Brazilian bocachico in captivity when hormonally induced to breed in the reproductive and non-reproductive seasons. During sampling spermiation was hormonally induced in breeders. The concentrations of total protein, glucose, fructose, triglyceride, calcium and chloride were evaluated with biochemical kits. The pH data (6.5 to 8.5) suggest semen requires alkaline conditions, as expected for freshwater fish. Seminal plasma contained more protein (1.51 ± 0.06 dL g-1), glucose (79.44 ± 1.88 mg dL-1) and triglycerides (61.59 ± 8.10 mg dL-1) in the non-reproductive than the reproductive season, but calcium ions (15.98 ± 1.02 mg dL-1) showed the opposite pattern. There was a significant seasonal difference in sperm morphology, with a higher percentage of normal sperm in the reproductive season. From these data it can be concluded that the physical, kinetic, morphological and biochemical characteristics of semen of captive Prochilodus brevis are influenced by reproductive season. | RESUMO O curimatã comum Prochilodus brevis caracteriza-se como um peixe reofílico. Na natureza, esses animais apresentam sazonalidade reprodutiva, porém em cativeiro, técnicas de indução hormonal, permitem a coleta de sêmen em diferentes períodos do ano. Objetivou-se comparar a cinética, a morfologia e a composição física e bioquímica do sêmen de curimatã comum induzidos à reprodução em cativeiro com uso de hormônio, na estação reprodutiva e não reprodutiva. As concentrações de proteínas totais, glicose, frutose, triglicerídeo, cálcio e cloreto foram avaliadas através de kits bioquímicos e analisadas em espectrofotômetro. Os dados de pH do sêmen, sugerem condição alcalina do sêmen dentro do esperado para peixes de água doce (6,5 a 8,5). A quantidade de proteínas totais (1,51 ± 0,06 g dL-1), glicose (79,44 ± 1,88 mg dL-1) e triglicerídeos (61,59 ± 8,10 mg dL-1) no plasma seminal apresentaram uma maior concentração na estação não reprodutiva. Enquanto que o oposto foi verificado para os íons cálcio (15,98 ± 1,02 mg dL-1). Houve diferença significativa, na morfologia do espermatozoide na estação reprodutiva, com uma porcentagem de espermatozoides normais superior em relação à estação não reprodutiva. Assim, pode-se concluir que características físicas, bioquímicas, cinéticas e morfológicas do sêmen de curimatã comum, mantidos em cativeiro, sofrem influência da estação reprodutiva.